RNF19A Antibody - #DF8717
| Product: | RNF19A Antibody |
| Catalog: | DF8717 |
| Description: | Rabbit polyclonal antibody to RNF19A |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 90 kDa; 91kD(Calculated). |
| Uniprot: | Q9NV58 |
| RRID: | AB_2841921 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8717, RRID:AB_2841921.
Fold/Unfold
AA032313; DKFZP566B1346; Dorfin; Double ring finger protein; Double ring-finger protein; E3 ubiquitin-protein ligase RNF19A; p38; p38 protein; Ring finger protein 19; RING finger protein 19A; Ring IBR ring domain containing protein Dorfin; RN19A_HUMAN; RNF19; RNF19A; Ubce7ip2; UIP117; XYbp;
Immunogens
Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system.
- Q9NV58 RN19A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQEQEIGFISKYNEGLCVNTDPVSILTSILDMSLHRQMGSDRDLQSSASSVSLPSVKKAPKKRRISIGSLFRRKKDNKRKSRELNGGVDGIASIESIHSEMCTDKNSIFSTNTSSDNGLTSISKQIGDFIECPLCLLRHSKDRFPDIMTCHHRSCVDCLRQYLRIEISESRVNISCPECTERFNPHDIRLILSDDVLMEKYEEFMLRRWLVADPDCRWCPAPDCGYAVIAFGCASCPKLTCGREGCGTEFCYHCKQIWHPNQTCDAARQERAQSLRLRTIRSSSISYSQESGAAADDIKPCPRCAAYIIKMNDGSCNHMTCAVCGCEFCWLCMKEISDLHYLSPSGCTFWGKKPWSRKKKILWQLGTLVGAPVGIALIAGIAIPAMIIGIPVYVGRKIHNRYEGKDVSKHKRNLAIAGGVTLSVIVSPVVAAVTVGIGVPIMLAYVYGVVPISLCRSGGCGVSAGNGKGVRIEFDDENDINVGGTNTAVDTTSVAEARHNPSIGEGSVGGLTGSLSASGSHMDRIGAIRDNLSETASTMALAGASITGSLSGSAMVNCFNRLEVQADVQKERYSLSGESGTVSLGTVSDNASTKAMAGSILNSYIPLDKEGNSMEVQVDIESKPSKFRHNSGSSSVDDGSATRSHAGGSSSGLPEGKSSATKWSKEATAGKKSKSGKLRKKGNMKINETREDMDAQLLEQQSTNSSEFEAPSLSDSMPSVADSHSSHFSEFSCSDLESMKTSCSHGSSDYHTRFATVNILPEVENDRLENSPHQCSISVVTQTASCSEVSQLNHIAEEHGNNGIKPNVDLYFGDALKETNNNHSHQTMELKVAIQTEI
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NV58 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S40 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| Y604 | Phosphorylation | Uniprot | |
| S631 | Phosphorylation | Uniprot | |
| S649 | Phosphorylation | Uniprot | |
| K657 | Ubiquitination | Uniprot | |
| Y750 | Phosphorylation | Uniprot | |
| Y811 | Phosphorylation | Uniprot | |
| T827 | Phosphorylation | Uniprot |
Research Backgrounds
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.
Membrane>Multi-pass membrane protein. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system.
Interacts with UBE2L3 and UBE2L6. Interacts with transcription factor Sp1. Interacts with VCP, CASR, SNCAIP and with some SOD1 variants which cause amyotrophic lateral sclerosis, but not with wild-type SOD1.
Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.
Belongs to the RBR family. RNF19 subfamily.
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