TF3C4 Antibody - #DF8729

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Product: TF3C4 Antibody
Catalog: DF8729
Description: Rabbit polyclonal antibody to TF3C4
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 92 kDa; 92kD(Calculated).
Uniprot: Q9UKN8
RRID: AB_2841933

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(90%), Horse(100%), Sheep(90%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
TF3C4 Antibody detects endogenous levels of total TF3C4.
RRID:
AB_2841933
Cite Format: Affinity Biosciences Cat# DF8729, RRID:AB_2841933.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.3.1.48; FLJ21002; General transcription factor 3C 4; General transcription factor 3C polypeptide 4; General transcription factor IIIC, polypeptide 4, 90kDa; GTF3C4; KAT12; MGC138450; OTTHUMP00000022434; TF3C delta; TF3C-delta; TF3C4_HUMAN; TFIII90; TFIIIC 90 kDa subunit; TFiiiC2 90; TFIIIC290; TFIIIC90; TFIIICdelta; Transcription factor IIIC 90 kDa subunit; Transcription factor IIIC subunit delta;

Immunogens

Immunogen:
Uniprot:

Q9UKN8(TF3C4_HUMAN) >>Visit HPA database.

Gene(ID):
GTF3C4(9329)
Sequence:
MNTADQARVGPADDGPAPSGEEEGEGGGEAGGKEPAADAAPGPSAAFRLMVTRREPAVKLQYAVSGLEPLAWSEDHRVSVSTARSIAVLELICDVHNPGQDLVIHRTSVPAPLNSCLLKVGSKTEVAECKEKFAASKDPTVSQTFMLDRVFNPEGKALPPMRGFKYTSWSPMGCDANGRCLLAALTMDNRLTIQANLNRLQWVQLVDLTEIYGERLYETSYRLSKNEAPEGNLGDFAEFQRRHSMQTPVRMEWSGICTTQQVKHNNECRDVGSVLLAVLFENGNIAVWQFQLPFVGKESISSCNTIESGITSPSVLFWWEYEHNNRKMSGLIVGSAFGPIKILPVNLKAVKGYFTLRQPVILWKEMDQLPVHSIKCVPLYHPYQKCSCSLVVAARGSYVFWCLLLISKAGLNVHNSHVTGLHSLPIVSMTADKQNGTVYTCSSDGKVRQLIPIFTDVALKFEHQLIKLSDVFGSVRTHGIAVSPCGAYLAIITTEGMINGLHPVNKNYQVQFVTLKTFEEAAAQLLESSVQNLFKQVDLIDLVRWKILKDKHIPQFLQEALEKKIESSGVTYFWRFKLFLLRILYQSMQKTPSEALWKPTHEDSKILLVDSPGMGNADDEQQEEGTSSKQVVKQGLQERSKEGDVEEPTDDSLPTTGDAGGREPMEEKLLEIQGKIEAVEMHLTREHMKRVLGEVYLHTWITENTSIPTRGLCNFLMSDEEYDDRTARVLIGHISKKMNKQTFPEHCSLCKEILPFTDRKQAVCSNGHIWLRCFLTYQSCQSLIYRRCLLHDSIARHPAPEDPDWIKRLLQSPCPFCDSPVF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
90
Sheep
90
Xenopus
90
Chicken
90
Zebrafish
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKN8 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S19 Phosphorylation
K33 Ubiquitination
K59 Ubiquitination
K119 Ubiquitination
S122 Phosphorylation
K123 Ubiquitination
K137 Ubiquitination
R149 Methylation
K156 Ubiquitination
K165 Methylation
K165 Ubiquitination
Y217 Phosphorylation
K225 Ubiquitination
K348 Ubiquitination
T355 Phosphorylation
K364 Ubiquitination
K375 Ubiquitination
Y380 Phosphorylation
Y383 Phosphorylation
Y398 Phosphorylation
K446 Ubiquitination
K460 Ubiquitination
K467 Ubiquitination
K551 Ubiquitination
K563 Ubiquitination
T571 Phosphorylation
K598 Ubiquitination
T600 Phosphorylation
S604 Phosphorylation
S611 Phosphorylation
K629 Ubiquitination
K633 Ubiquitination
S640 Phosphorylation
K641 Ubiquitination
S652 Phosphorylation
T656 Phosphorylation
K668 Ubiquitination
K740 Ubiquitination
K760 Ubiquitination
K807 Ubiquitination

Research Backgrounds

Function:

Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Interacts with BRF1, GTF3C1, GTF3C2, GTF3C5, GTF3C6, POLR3C and POLR3F.

Family&Domains:

Belongs to the TFIIIC subunit 4 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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