CHD1 Antibody - #DF8753

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Product: CHD1 Antibody
Catalog: DF8753
Description: Rabbit polyclonal antibody to CHD1
Application: WB
Reactivity: Human, Mouse
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 197kDa; 197kD(Calculated).
Uniprot: O14646
RRID: AB_2841957

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
CHD1 Antibody detects endogenous levels of total CHD1.
RRID:
AB_2841957
Cite Format: Affinity Biosciences Cat# DF8753, RRID:AB_2841957.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent helicase CHD 1; ATP-dependent helicase CHD1; CHD 1; CHD-1; Chd1; CHD1_HUMAN; Chromodomain helicase DNA binding protein 1; Chromodomain-helicase-DNA-binding protein 1; DKFZp686E2337; OTTHUMP00000222625;

Immunogens

Immunogen:
Uniprot:

O14646(CHD1_HUMAN) >>Visit HPA database.

Gene(ID):
CHD1(1105)
Expression:
O14646 CHD1_HUMAN:

Expressed in many tissues including in the brain, where the highest level of expression is found in the cerebellum and basal ganglia.

Sequence:
MNGHSDEESVRNSSGESSQSDDDSGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSESGSQSESESDTSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAILKKQQQQQQQQQHQASSNSGSEEDSSSSEDSDDSSSEVKRKKHKDEDWQMSGSGSPSQSGSDSESEEEREKSSCDETESDYEPKNKVKSRKPQNRSKSKNGKKILGQKKRQIDSSEEDDDEEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEEEEFETIERFMDCRIGRKGATGATTTIYAVEADGDPNAGFEKNKEPGEIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGYPDYYCKWQGLPYSECSWEDGALISKKFQACIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWTHHQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLTVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEDQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSISEGKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCIKALKDSSSGTERTGGRLGKVKGPTFRISGVQVNAKLVISHEEELIPLHKSIPSDPEERKQYTIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSRDLAKKEALSGAGSSKRRKARAKKNKAMKSIKVKEEIKSDSSPLPSEKSDEDDDKLSESKSDGRERSKKSSVSDAPVHITASGEPVPISEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNSDQNSNLNPHVIRNPDVERLKENTNHDDSSRDSYSSDRHLTQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSLKDRSHSDHRSHSDHRLHSDHRSSSEYTHHKSSRDYRYHSDWQMDHRASSSGPRSPLDQRSPYGSRSPFEHSVEHKSTPEHTWSSRKT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
83
Zebrafish
82
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14646 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
K88 Ubiquitination
S89 Phosphorylation
S90 Phosphorylation
S92 Phosphorylation
S173 Phosphorylation
S174 Phosphorylation
T178 Phosphorylation
S180 Phosphorylation
Y182 Phosphorylation
S215 Phosphorylation
S216 Phosphorylation
Y225 Phosphorylation
S232 Phosphorylation
T237 Phosphorylation
S241 Phosphorylation
T250 Phosphorylation
S252 Phosphorylation
K285 Ubiquitination
K311 Ubiquitination
K339 Ubiquitination
K348 Acetylation
K364 Ubiquitination
S367 Phosphorylation
K386 Ubiquitination
Y388 Phosphorylation
S398 Phosphorylation
K401 Ubiquitination
K412 Ubiquitination
K431 Ubiquitination
K447 Ubiquitination
T449 Phosphorylation
K455 Ubiquitination
K468 Ubiquitination
S471 Phosphorylation
Y472 Phosphorylation
K620 Ubiquitination
K628 Ubiquitination
K634 Ubiquitination
S667 Phosphorylation
K677 Ubiquitination
Y681 Phosphorylation
S705 Phosphorylation
S719 Phosphorylation
S738 Phosphorylation
T745 Phosphorylation
Y823 Phosphorylation
K915 Ubiquitination
K945 Ubiquitination
T958 Phosphorylation
K962 Ubiquitination
S966 Phosphorylation
K997 Ubiquitination
S1025 Phosphorylation
S1040 Phosphorylation
K1041 Ubiquitination
K1062 Ubiquitination
Y1068 Phosphorylation
S1081 Phosphorylation
S1085 Phosphorylation
S1090 Phosphorylation
Y1095 Phosphorylation
S1096 Phosphorylation
S1098 Phosphorylation
S1100 Phosphorylation
S1102 Phosphorylation
K1124 Methylation
K1124 Ubiquitination
S1127 Phosphorylation
K1160 Ubiquitination
S1161 Phosphorylation
S1183 Phosphorylation
S1184 Phosphorylation
T1187 Phosphorylation
T1190 Phosphorylation
K1226 Ubiquitination
S1230 Phosphorylation
K1236 Ubiquitination
S1283 Phosphorylation
T1285 Phosphorylation
K1320 Ubiquitination
S1324 Phosphorylation
S1328 Phosphorylation
S1329 Phosphorylation
K1338 Acetylation
K1340 Acetylation
K1343 Acetylation
K1346 Acetylation
S1353 Phosphorylation
S1355 Phosphorylation
S1356 Phosphorylation
S1360 Phosphorylation
S1363 Phosphorylation
S1371 Phosphorylation
S1373 Phosphorylation
S1385 Phosphorylation
S1387 Phosphorylation
S1396 Phosphorylation
S1403 Phosphorylation
S1406 Phosphorylation
K1436 Ubiquitination
R1491 Methylation
S1511 Phosphorylation
S1539 Phosphorylation
S1540 Phosphorylation
S1543 Phosphorylation
S1546 Phosphorylation
S1578 Phosphorylation
S1580 Phosphorylation
Y1591 Phosphorylation
S1595 Phosphorylation
S1599 Phosphorylation
S1622 Phosphorylation
S1662 Phosphorylation
S1671 Phosphorylation
S1672 Phosphorylation
S1673 Phosphorylation
S1677 Phosphorylation
S1683 Phosphorylation
Y1685 Phosphorylation
S1687 Phosphorylation
S1689 Phosphorylation
S1694 Phosphorylation
S1699 Phosphorylation
T1700 Phosphorylation

Research Backgrounds

Function:

ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells (By similarity).

Subcellular Location:

Nucleus. Cytoplasm.
Note: Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during telophase-cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in many tissues including in the brain, where the highest level of expression is found in the cerebellum and basal ganglia.

Subunit Structure:

Component of the SAGA complex (By similarity). Interacts with BCLAF1, NCoR, SRP20 and SAFB (By similarity). Specifically interacts with methylated H3K4me2 and H3K4me3. Interacts with the FACT complex, the PAF complex and the U2 snRNP. Interacts directly with PAF1, SFA3A1, SFA3A2, SFA3A3, SNF2 and SSRP1.

Family&Domains:

The 2 chromodomains are involved in the binding to the histone H3 methyllysine at position 4 (H3K4me3).

Belongs to the SNF2/RAD54 helicase family.

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