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NKRF Antibody - Western blot analysis of extracts from Mouse brain, using NKRF Antibody.

Product:	NKRF Antibody
Catalog:	DF8755
Description:	Rabbit polyclonal antibody to NKRF
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	79 kDa; 78kD(Calculated).
Uniprot:	O15226
RRID:	AB_2841959

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(92%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

NKRF Antibody detects endogenous levels of total NKRF.

RRID:

AB_2841959
Cite Format: Affinity Biosciences Cat# DF8755, RRID:AB_2841959.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ITBA4; NF kappa B repressing factor; NFKB repressing factor; NRF; Transcription factor NRF; NF-kappa-B-repressing factor; NKRF; NKRF_HUMAN;Protein ITBA4; NKRF;

Immunogens

Immunogen:

Uniprot:

O15226(NKRF_HUMAN) >>Visit HPA database.

Gene(ID):

NKRF(55922)

Expression:

O15226 NKRF_HUMAN:

Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney.

Sequence:

O15226 NKRF_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Rabbit

100

Bovine

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15226 As Substrate

O15226

Site	PTM Type	Source
S18	Phosphorylation	Uniprot
Y19	Phosphorylation	Uniprot
K25	Ubiquitination	Uniprot
S35	Phosphorylation	Uniprot
T36	Phosphorylation	Uniprot
S65	Phosphorylation	Uniprot
S67	Phosphorylation	Uniprot
K68	Sumoylation	Uniprot
K68	Ubiquitination	Uniprot
Y76	Phosphorylation	Uniprot
K81	Ubiquitination	Uniprot
S91	Phosphorylation	Uniprot
K176	Ubiquitination	Uniprot
K183	Ubiquitination	Uniprot
K196	Ubiquitination	Uniprot
K211	Ubiquitination	Uniprot
Y216	Phosphorylation	Uniprot
K222	Ubiquitination	Uniprot
K234	Ubiquitination	Uniprot
T237	Phosphorylation	Uniprot
Y240	Phosphorylation	Uniprot
Y250	Phosphorylation	Uniprot
T252	Phosphorylation	Uniprot
T253	Phosphorylation	Uniprot
Y255	Phosphorylation	Uniprot
K259	Ubiquitination	Uniprot
K272	Ubiquitination	Uniprot
K276	Ubiquitination	Uniprot
K288	Methylation	Uniprot
S325	Phosphorylation	Uniprot
S333	Phosphorylation	Uniprot
Y366	Phosphorylation	Uniprot
Y368	Phosphorylation	Uniprot
K398	Ubiquitination	Uniprot
K407	Sumoylation	Uniprot
K407	Ubiquitination	Uniprot
K411	Ubiquitination	Uniprot
K420	Ubiquitination	Uniprot
S429	Phosphorylation	Uniprot
S486	Phosphorylation	Uniprot
K500	Sumoylation	Uniprot
Y501	Phosphorylation	Uniprot
K509	Ubiquitination	Uniprot
T510	Phosphorylation	Uniprot
K513	Ubiquitination	Uniprot
T514	Phosphorylation	Uniprot
T517	Phosphorylation	Uniprot
K523	Sumoylation	Uniprot
K523	Ubiquitination	Uniprot
K524	Sumoylation	Uniprot
K524	Ubiquitination	Uniprot
K546	Methylation	Uniprot
K546	Sumoylation	Uniprot
K546	Ubiquitination	Uniprot
K550	Sumoylation	Uniprot
K550	Ubiquitination	Uniprot
K561	Sumoylation	Uniprot
K561	Ubiquitination	Uniprot
T565	Phosphorylation	Uniprot
S572	Phosphorylation	Uniprot
K583	Sumoylation	Uniprot
K583	Ubiquitination	Uniprot
S616	Phosphorylation	Uniprot
S618	Phosphorylation	Uniprot
T620	Phosphorylation	Uniprot
S625	Phosphorylation	Uniprot
K634	Ubiquitination	Uniprot
K642	Ubiquitination	Uniprot
K648	Acetylation	Uniprot
R656	Methylation	Uniprot
K666	Methylation	Uniprot
K666	Ubiquitination	Uniprot
K674	Sumoylation	Uniprot
K674	Ubiquitination	Uniprot
Y682	Phosphorylation	Uniprot

Research Backgrounds

O15226_NKRF_HUMAN

Function:

Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.

Subcellular Location:

Nucleus>Nucleolus.

Tissue Specificity:

Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney.

Subunit Structure:

Interacts with NF-kappa-B. Interacts with XRN2.

References

1). circ_0000638 inhibits neodymium oxide-induced bronchial epithelial cell inflammation through the miR-498–5p/NF-κB axis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2020 (PubMed: 32199215) [IF=6.2]

Application: WB Species: human Sample: neodymium oxide-treated 16HBE cells

Fig. 4. |Neodymium oxide activated the NF-κB signaling pathway by inhibiting NKRF expression, and circ_0000638 inhibited the activation of the NF-κB signaling pathway. (A, B) Expression of NKRF, p-IκB, and p-p65 protein in neodymium oxide-treated 16HBE cells detected by Western blot. ***p < 0.001:compared with control.

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NKRF Antibody - #DF8755