Product: SOX12 Antibody
Catalog: DF8757
Description: Rabbit polyclonal antibody to SOX12
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Dog
Mol.Wt.: 34 kDa; 34kD(Calculated).
Uniprot: O15370
RRID: AB_2841961

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SOX12 Antibody detects endogenous levels of total SOX12.
RRID:
AB_2841961
Cite Format: Affinity Biosciences Cat# DF8757, RRID:AB_2841961.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Homo sapiens SRY (sex determining region Y) box 12; Protein SOX-22; SOX 12; SOX 12 protein; SOX 22; SOX 22 protein; sox12; SOX12 protein; SOX12_HUMAN; SOX22; SOX22 protein; SRY (sex determining region Y) box 12; SRY (sex determining region Y) box 22; SRY box 12; SRY box 22; SRY related HMG box gene 22; Transcription factor SOX 12; Transcription factor Sox-12;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O15370 SOX12_HUMAN:

Expressed most abundantly in the CNS. Also expressed in fetal brain and kidney and adult heart, pancreas, testis and ovary. Other tissues were only weakly positive.

Sequence:
MVQQRGARAKRDGGPPPPGPGPAEEGAREPGWCKTPSGHIKRPMNAFMVWSQHERRKIMDQWPDMHNAEISKRLGRRWQLLQDSEKIPFVREAERLRLKHMADYPDYKYRPRKKSKGAPAKARPRPPGGSGGGSRLKPGPQLPGRGGRRAAGGPLGGGAAAPEDDDEDDDEELLEVRLVETPGRELWRMVPAGRAARGQAERAQGPSGEGAAAAAAASPTPSEDEEPEEEEEEAAAAEEGEEETVASGEESLGFLSRLPPGPAGLDCSALDRDPDLQPPSGTSHFEFPDYCTPEVTEMIAGDWRPSSIADLVFTY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15370 As Substrate

Site PTM Type Enzyme
K99 Ubiquitination
K108 Ubiquitination
K137 Ubiquitination
R145 Methylation
R148 Methylation
T181 Phosphorylation

Research Backgrounds

Function:

Binds to the sequence 5'-AACAAT-3'.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed most abundantly in the CNS. Also expressed in fetal brain and kidney and adult heart, pancreas, testis and ovary. Other tissues were only weakly positive.

Restrictive clause

 

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For Research Use Only.
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