CDKL5 Antibody - #DF8765
(1)
| Product: | CDKL5 Antibody |
| Catalog: | DF8765 |
| Description: | Rabbit polyclonal antibody to CDKL5 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 116kDa; 116kD(Calculated). |
| Uniprot: | O76039 |
| RRID: | AB_2841969 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
CDKL5 Antibody detects endogenous levels of total CDKL5.
RRID:
AB_2841969
Cite Format: Affinity Biosciences Cat# DF8765, RRID:AB_2841969.
Cite Format: Affinity Biosciences Cat# DF8765, RRID:AB_2841969.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Cdkl5; CDKL5_HUMAN; Cyclin dependent kinase 5 transcript; Cyclin-dependent kinase-like 5; EIEE2; ISSX; Serine/threonine kinase 9; Serine/threonine-protein kinase 9; Stk9;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
O76039 CDKL5_HUMAN:
Expressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis.
Sequence:
- O76039 CDKL5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGNNANYTEYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSVLLDLMKNLLKLDPADRYLTEQCLNHPTFQTQRLLDRSPSRSAKRKPYHVESSTLSNRNQAGKSTALQSHHRSNSKDIQNLSVGLPRADEGLPANESFLNGNLAGASLSPLHTKTYQASSQPGSTSKDLTNNNIPHLLSPKEAKSKTEFDFNIDPKPSEGPGTKYLKSNSRSQQNRHSFMESSQSKAGTLQPNEKQSRHSYIDTIPQSSRSPSYRTKAKSHGALSDSKSVSNLSEARAQIAEPSTSRYFPSSCLDLNSPTSPTPTRHSDTRTLLSPSGRNNRNEGTLDSRRTTTRHSKTMEELKLPEHMDSSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVTRDKVRAKGLDGSLSIGQGMAARANSLQLLSPQPGEQLPPEMTVARSSVKETSREGTSSFHTRQKSEGGVYHDPHSDDGTAPKENRHLYNDPVPRRVGSFYRVPSPRPDNSFHENNVSTRVSSLPSESSSGTNHSKRQPAFDPWKSPENISHSEQLKEKEKQGFFRSMKKKKKKSQTVPNSDSPDLLTLQKSIHSASTPSSRPKEWRPEKISDLQTQSQPLKSLRKLLHLSSASNHPASSDPRFQPLTAQQTKNSFSEIRIHPLSQASGGSSNIRQEPAPKGRPALQLPDGGCDGRRQRHHSGPQDRRFMLRTTEQQGEYFCCGDPKKPHTPCVPNRALHRPISSPAPYPVLQVRGTSMCPTLQVRGTDAFSCPTQQSGFSFFVRHVMREALIHRAQVNQAALLTYHENAALTGK
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
100
Horse
100
Pig
92
Bovine
92
Sheep
92
Dog
92
Xenopus
67
Zebrafish
58
Chicken
58
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O76039 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K2 | Acetylation | Uniprot | |
| K12 | Acetylation | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| Y168 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | O76039 (CDKL5) | Uniprot |
| Y171 | Phosphorylation | O76039 (CDKL5) | Uniprot |
| Y262 | Phosphorylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| Y286 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| Y482 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S512 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S526 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| T531 | Phosphorylation | Uniprot | |
| T533 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| S603 | Phosphorylation | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| S646 | Phosphorylation | Uniprot | |
| T658 | Phosphorylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| Y686 | Phosphorylation | Uniprot | |
| Y704 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| K760 | Ubiquitination | Uniprot | |
| S761 | Phosphorylation | Uniprot | |
| T813 | Phosphorylation | Uniprot | |
| T831 | Phosphorylation | Uniprot | |
| S833 | Phosphorylation | Uniprot | |
| S870 | Phosphorylation | Uniprot | |
| T983 | Phosphorylation | Uniprot | |
| S987 | Phosphorylation | Uniprot |
PTMs - O76039 As Enzyme
Research Backgrounds
Function:
Mediates phosphorylation of MECP2. May regulate ciliogenesis.
PTMs:
Autophosphorylated.
Subcellular Location:
Nucleus. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Tissue Specificity:
Expressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis.
Subunit Structure:
Interacts with MECP2.
Family&Domains:
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
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