KLF8 Antibody - #DF8769
| Product: | KLF8 Antibody |
| Catalog: | DF8769 |
| Description: | Rabbit polyclonal antibody to KLF8 |
| Application: | WB |
| Reactivity: | Human, Rat, Monkey |
| Prediction: | Pig, Horse, Rabbit |
| Mol.Wt.: | 39 kDa; 39kD(Calculated). |
| Uniprot: | O95600 |
| RRID: | AB_2841973 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8769, RRID:AB_2841973.
Fold/Unfold
Basic krueppel-like factor 3; BKLF3; DXS741; KLF8; KLF8_HUMAN; Krueppel-like factor 8; Kruppel like factor 8; MGC138314; Zinc finger protein 741; ZNF741;
Immunogens
- O95600 KLF8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVDMDKLINNLEVQLNSEGGSMQVFKQVTASVRNRDPPEIEYRSNMTSPTLLDANPMENPALFNDIKIEPPEELLASDFSLPQVEPVDLSFHKPKAPLQPASMLQAPIRPPKPQSSPQTLVVSTSTSDMSTSANIPTVLTPGSVLTSSQSTGSQQILHVIHTIPSVSLPNKMGGLKTIPVVVQSLPMVYTTLPADGGPAAITVPLIGGDGKNAGSVKVDPTSMSPLEIPSDSEESTIESGSSALQSLQGLQQEPAAMAQMQGEESLDLKRRRIHQCDFAGCSKVYTKSSHLKAHRRIHTGEKPYKCTWDGCSWKFARSDELTRHFRKHTGIKPFRCTDCNRSFSRSDHLSLHRRRHDTM
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95600 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S48 | Phosphorylation | Uniprot | |
| K67 | Acetylation | Uniprot | |
| K67 | Sumoylation | Uniprot | |
| K93 | Acetylation | Uniprot | |
| K95 | Acetylation | Uniprot | |
| T299 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot |
Research Backgrounds
Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).
Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location.
Nucleus.
Ubiquitous.
Interacts with corepressor CtBP2. Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8.
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In KLF8, the motif is inactive.
Belongs to the Sp1 C2H2-type zinc-finger protein family.
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For Research Use Only.
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