AKAP12 Antibody - #DF8813

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Product: AKAP12 Antibody
Catalog: DF8813
Description: Rabbit polyclonal antibody to AKAP12
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Rabbit, Dog
Mol.Wt.: 191 kDa; 191kD(Calculated).
Uniprot: Q02952
RRID: AB_2842010

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Rabbit(83%), Dog(86%)
Clonality:
Polyclonal
Specificity:
AKAP12 Antibody detects endogenous levels of total AKAP12.
RRID:
AB_2842010
Cite Format: Affinity Biosciences Cat# DF8813, RRID:AB_2842010.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A kinase (PRKA) anchor protein (gravin) 12; A kinase Anchor Protein 12; A kinase anchor protein 250kDa; A-kinase anchor protein 12; A-kinase anchor protein 250 kDa; AKA12_HUMAN; AKAP 12; AKAP 250; AKAP-12; Akap12; AKAP250; DKFZp686M0430; DKFZp686O0331; FLJ20945; FLJ97621; Gercelin; Gravin; Kinase scaffold protein gravin; Myasthenia gravis autoantigen; Myasthenia gravis autoantigen gravin; OTTHUMP00000017426; OTTHUMP00000017427; Src-Suppressed C Kinase Substrate; Srcs5; SSeCKS; Tsga12;

Immunogens

Immunogen:
Uniprot:

Q02952(AKA12_HUMAN) >>Visit HPA database.

Gene(ID):
AKAP12(9590)
Expression:
Q02952 AKA12_HUMAN:

Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.

Sequence:
MGAGSSTEQRSPEQPPEGSSTPAEPEPSGGGPSAEAAPDTTADPAIAASDPATKLLQKNGQLSTINGVAEQDELSLQEGDLNGQKGALNGQGALNSQEEEEVIVTEVGQRDSEDVSKRDSDKEMATKSAVVHDITDDGQEETPEIIEQIPSSESNLEELTQPTESQANDIGFKKVFKFVGFKFTVKKDKTEKPDTVQLLTVKKDEGEGAAGAGDHKDPSLGAGEAASKESEPKQSTEKPEETLKREQSHAEISPPAESGQAVEECKEEGEEKQEKEPSKSAESPTSPVTSETGSTFKKFFTQGWAGWRKKTSFRKPKEDEVEASEKKKEQEPEKVDTEEDGKAEVASEKLTASEQAHPQEPAESAHEPRLSAEYEKVELPSEEQVSGSQGPSEEKPAPLATEVFDEKIEVHQEEVVAEVHVSTVEERTEEQKTEVEETAGSVPAEELVEMDAEPQEAEPAKELVKLKETCVSGEDPTQGADLSPDEKVLSKPPEGVVSEVEMLSSQERMKVQGSPLKKLFTSTGLKKLSGKKQKGKRGGGDEESGEHTQVPADSPDSQEEQKGESSASSPEEPEEITCLEKGLAEVQQDGEAEEGATSDGEKKREGVTPWASFKKMVTPKKRVRRPSESDKEDELDKVKSATLSSTESTASEMQEEMKGSVEEPKPEEPKRKVDTSVSWEALICVGSSKKRARRGSSSDEEGGPKAMGGDHQKADEAGKDKETGTDGILAGSQEHDPGQGSSSPEQAGSPTEGEGVSTWESFKRLVTPRKKSKSKLEEKSEDSIAGSGVEHSTPDTEPGKEESWVSIKKFIPGRRKKRPDGKQEQAPVEDAGPTGANEDDSDVPAVVPLSEYDAVEREKMEAQQAQKSAEQPEQKAATEVSKELSESQVHMMAAAVADGTRAATIIEERSPSWISASVTEPLEQVEAEAALLTEEVLEREVIAEEEPPTVTEPLPENREARGDTVVSEAELTPEAVTAAETAGPLGAEEGTEASAAEETTEMVSAVSQLTDSPDTTEEATPVQEVEGGVPDIEEQERRTQEVLQAVAEKVKEESQLPGTGGPEDVLQPVQRAEAERPEEQAEASGLKKETDVVLKVDAQEAKTEPFTQGKVVGQTTPESFEKAPQVTESIESSELVTTCQAETLAGVKSQEMVMEQAIPPDSVETPTDSETDGSTPVADFDAPGTTQKDEIVEIHEENEVASGTQSGGTEAEAVPAQKERPPAPSSFVFQEETKEQSKMEDTLEHTDKEVSVETVSILSKTEGTQEADQYADEKTKDVPFFEGLEGSIDTGITVSREKVTEVALKGEGTEEAECKKDDALELQSHAKSPPSPVEREMVVQVEREKTEAEPTHVNEEKLEHETAVTVSEEVSKQLLQTVNVPIIDGAKEVSSLEGSPPPCLGQEEAVCTKIQVQSSEASFTLTAAAEEEKVLGETANILETGETLEPAGAHLVLEEKSSEKNEDFAAHPGEDAVPTGPDCQAKSTPVIVSATTKKGLSSDLEGEKTTSLKWKSDEVDEQVACQEVKVSVAIEDLEPENGILELETKSSKLVQNIIQTAVDQFVRTEETATEMLTSELQTQAHVIKADSQDAGQETEKEGEEPQASAQDETPITSAKEESESTAVGQAHSDISKDMSEASEKTMTVEVEGSTVNDQQLEEVVLPSEEEGGGAGTKSVPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKSELTES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
86
Rabbit
83
Pig
71
Horse
71
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02952 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
T7 Phosphorylation
S20 Phosphorylation
K54 Ubiquitination
S75 Phosphorylation
S96 Phosphorylation
S120 Phosphorylation
K122 Ubiquitination
S154 Phosphorylation
K177 Ubiquitination
K182 Ubiquitination
T184 Phosphorylation
K187 Ubiquitination
K189 Ubiquitination
K192 Ubiquitination
K203 Ubiquitination
S219 Phosphorylation
K228 Ubiquitination
S235 Phosphorylation
T236 Phosphorylation
S248 Phosphorylation
S253 Phosphorylation
K279 Ubiquitination
S280 Phosphorylation
S283 Phosphorylation
T285 Phosphorylation
S286 Phosphorylation P28482 (MAPK1)
T289 Phosphorylation
S290 Phosphorylation
S294 Phosphorylation
K297 Acetylation
K297 Ubiquitination
K298 Acetylation
K298 Ubiquitination
T311 Phosphorylation
S312 Phosphorylation
K315 Ubiquitination
K317 Ubiquitination
K342 Ubiquitination
S347 Phosphorylation
S353 Phosphorylation
S364 Phosphorylation
S371 Phosphorylation
Y374 Phosphorylation
K376 Ubiquitination
S381 Phosphorylation
S386 Phosphorylation
S388 Phosphorylation
S392 Phosphorylation
K395 Ubiquitination
K432 Ubiquitination
S441 Phosphorylation
K461 Ubiquitination
K467 Ubiquitination
T469 Phosphorylation
C470 S-Nitrosylation
S472 Phosphorylation
T477 Phosphorylation
S483 Phosphorylation
K491 Ubiquitination
S505 Phosphorylation
S514 Phosphorylation
T521 Phosphorylation
S522 Phosphorylation
K526 Acetylation
K526 Ubiquitination
S529 Phosphorylation
R537 Methylation
S544 Phosphorylation
S554 Phosphorylation
S557 Phosphorylation
S566 Phosphorylation
S568 Phosphorylation
S569 Phosphorylation
T597 Phosphorylation
S598 Phosphorylation
K602 Ubiquitination
T608 Phosphorylation
S612 Phosphorylation
K614 Acetylation
K615 Acetylation
T618 Phosphorylation
S627 Phosphorylation P17612 (PRKACA)
S629 Phosphorylation
K639 Ubiquitination
S640 Phosphorylation
T642 Phosphorylation
S644 Phosphorylation
S645 Phosphorylation
T646 Phosphorylation
S648 Phosphorylation
T649 Phosphorylation
S651 Phosphorylation
S660 Phosphorylation
K670 Ubiquitination
K672 Ubiquitination
S687 Phosphorylation
K689 Ubiquitination
S696 Phosphorylation P17612 (PRKACA)
S697 Phosphorylation
S698 Phosphorylation P17612 (PRKACA)
S732 Phosphorylation
S749 Phosphorylation
S761 Phosphorylation
K763 Ubiquitination
T767 Phosphorylation P06493 (CDK1)
K771 Acetylation
S772 Phosphorylation P17612 (PRKACA)
K775 Acetylation
S780 Phosphorylation
S783 Phosphorylation
S787 Phosphorylation
S792 Phosphorylation
T793 Phosphorylation
T796 Phosphorylation
K800 Ubiquitination
S803 Phosphorylation
S806 Phosphorylation
K808 Ubiquitination
S841 Phosphorylation
Y852 Phosphorylation
K859 Ubiquitination
K867 Ubiquitination
S868 Phosphorylation
K875 Ubiquitination
S881 Phosphorylation
K882 Ubiquitination
S887 Phosphorylation
T904 Phosphorylation
S910 Phosphorylation
S1012 Phosphorylation
T1016 Phosphorylation
T1039 Phosphorylation
K1049 Ubiquitination
K1051 Sumoylation
K1051 Ubiquitination
S1054 Phosphorylation
K1087 Acetylation
K1087 Ubiquitination
K1088 Acetylation
K1102 Ubiquitination
K1110 Ubiquitination
T1116 Phosphorylation
S1119 Phosphorylation
T1127 Phosphorylation
C1139 S-Nitrosylation
S1226 Phosphorylation
K1234 Ubiquitination
T1242 Phosphorylation
T1246 Phosphorylation
S1251 Phosphorylation
S1256 Phosphorylation
S1259 Phosphorylation
K1260 Ubiquitination
T1264 Phosphorylation
Y1270 Phosphorylation
K1274 Ubiquitination
S1287 Phosphorylation
C1314 S-Nitrosylation
K1316 Ubiquitination
S1324 Phosphorylation
S1328 Phosphorylation
S1331 Phosphorylation
S1390 Phosphorylation
S1391 Phosphorylation
S1395 Phosphorylation
S1415 Phosphorylation
K1456 Ubiquitination
C1479 S-Nitrosylation
T1484 Phosphorylation
K1493 Acetylation
K1493 Ubiquitination
S1497 Phosphorylation
S1498 Phosphorylation
S1512 Phosphorylation
C1521 S-Nitrosylation
K1548 Ubiquitination
T1564 Phosphorylation
K1584 Ubiquitination
S1587 Phosphorylation
T1594 Phosphorylation
S1628 Phosphorylation
S1631 Phosphorylation
S1635 Phosphorylation
S1663 Phosphorylation
S1674 Phosphorylation
K1690 Ubiquitination
S1691 Phosphorylation
K1700 Ubiquitination
S1712 Phosphorylation
T1717 Phosphorylation
S1720 Phosphorylation
T1724 Phosphorylation
K1725 Ubiquitination
S1727 Phosphorylation
K1750 Ubiquitination
T1760 Phosphorylation
K1769 Ubiquitination
S1774 Phosphorylation
K1776 Ubiquitination
S1777 Phosphorylation

Research Backgrounds

Function:

Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).

Subcellular Location:

Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. Membrane>Lipid-anchor.
Note: May be part of the cortical cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.

Subunit Structure:

Binds to dimeric RII-alpha regulatory subunit of PKC.

Family&Domains:

Polybasic regions located between residues 266 and 557 are involved in binding PKC.

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