DMP1 Antibody - #DF8825

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Product: DMP1 Antibody
Catalog: DF8825
Description: Rabbit polyclonal antibody to DMP1
Application: WB IHC
Reactivity: Human, Mouse, Monkey
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 56 kDa, 80 kDa; 56kD(Calculated).
Uniprot: Q13316
RRID: AB_2842022

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DMP1 Antibody detects endogenous levels of total DMP1.
RRID:
AB_2842022
Cite Format: Affinity Biosciences Cat# DF8825, RRID:AB_2842022.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHP; ARHR; AV020965; Dentin matrix acidic phosphoprotein 1; Dentin matrix protein 1; DENTMAT; DMP 1; Dmp; MGC130441; PP; Serine rich acidic phosphoprotein;

Immunogens

Immunogen:
Uniprot:

Q13316(DMP1_HUMAN) >>Visit HPA database.

Gene(ID):
DMP1(1758)
Expression:
Q13316 DMP1_HUMAN:

Expressed in tooth particularly in odontoblast, ameloblast and cementoblast.

Sequence:
MKISILLMFLWGLSCALPVTRYQNNESEDSEEWKGHLAQAPTPPLESSESSEGSKVSSEEQANEDPSDSTQSEEGLGSDDHQYIYRLAGGFSRSTGKGGDDKDDDEDDSGDDTFGDDDSGPGPKDRQEGGNSRLGSDEDSDDTIQASEESAPQGQDSAQDTTSESRELDNEDRVDSKPEGGDSTQESESEEHWVGGGSDGESSHGDGSELDDEGMQSDDPESIRSERGNSRMNSAGMKSKESGENSEQANTQDSGGSQLLEHPSRKIFRKSRISEEDDRSELDDNNTMEEVKSDSTENSNSRDTGLSQPRRDSKGDSQEDSKENLSQEESQNVDGPSSESSQEANLSSQENSSESQEEVVSESRGDNPDPTTSYVEDQEDSDSSEEDSSHTLSHSKSESREEQADSESSESLNFSEESPESPEDENSSSQEGLQSHSSSAESQSEESHSEEDDSDSQDSSRSKEDSNSTESKSSSEEDGQLKNIEIESRKLTVDAYHNKPIGDQDDNDCQDGY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
56
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13316 As Substrate

Site PTM Type Enzyme
K177 Sumoylation
S307 Phosphorylation

Research Backgrounds

Function:

May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.

PTMs:

Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif.

Subcellular Location:

Nucleus. Cytoplasm. Secreted>Extracellular space>Extracellular matrix.
Note: In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in tooth particularly in odontoblast, ameloblast and cementoblast.

Subunit Structure:

Interacts with importin alpha.

References

1). Gut microbial alterations in arginine metabolism determine bone mechanical adaptation. Cell metabolism, 2024 (PubMed: 38718794) [IF=27.7]
2). lncRNA SNHG1 regulates odontogenic differentiation of human dental pulp stem cells via miR-328-3p/Wnt/β-catenin pathway. Stem Cell Research & Therapy, 2022 (PubMed: 35841022) [IF=7.5]

Application: WB    Species: Human    Sample: hDPSCs

Fig. 2 SNHG1 expression in hDPSCs during the odontogenic differentiation and SNHG1 governs the odontogenic differentiation of hDPSCs. A Relative ALP, DMP-1, DSPP and SNHG1 expressions were measured by qRT-PCR at day 0, 3, and 7 under the mineralization induction condition. GAPDH was used for normalization. B The transfection efficacy of SNHG1 was measured by qRT-PCR in SNHG1 group and si-SNHG1 group, with GAPDH as a control. C The proliferation ability of hDPSCs was measured by CCK-8. D-G After SNHG1 overexpression or silence, relative ALP, DMP-1 and DSPP expression were measured by qRT-PCR and ALP, DMP-1 and DSPP expression were measured by Western blot at day 5 under the mineralization induction condition. H At 7 days after odontogenic induction, hDPSCs were performed with ALP staining and activity. I On the 14th day, Alizarin Red staining and quantification were performed. J The expression of DMP-1 and DSPP in transfected hDPSCs were also showed by Immunofluorescence Staining. Scale bar = 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001

Application: IF/ICC    Species: Human    Sample: hDPSCs

Fig. 2 SNHG1 expression in hDPSCs during the odontogenic differentiation and SNHG1 governs the odontogenic differentiation of hDPSCs. A Relative ALP, DMP-1, DSPP and SNHG1 expressions were measured by qRT-PCR at day 0, 3, and 7 under the mineralization induction condition. GAPDH was used for normalization. B The transfection efficacy of SNHG1 was measured by qRT-PCR in SNHG1 group and si-SNHG1 group, with GAPDH as a control. C The proliferation ability of hDPSCs was measured by CCK-8. D-G After SNHG1 overexpression or silence, relative ALP, DMP-1 and DSPP expression were measured by qRT-PCR and ALP, DMP-1 and DSPP expression were measured by Western blot at day 5 under the mineralization induction condition. H At 7 days after odontogenic induction, hDPSCs were performed with ALP staining and activity. I On the 14th day, Alizarin Red staining and quantification were performed. J The expression of DMP-1 and DSPP in transfected hDPSCs were also showed by Immunofluorescence Staining. Scale bar = 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001
3). FTO Positively Regulates Odontoblastic Differentiation via SMOC2 in Human Stem Cells from the Apical Papilla under Inflammatory Microenvironment. International journal of molecular sciences, 2024 (PubMed: 38612855) [IF=5.6]

Application: WB    Species: Human    Sample: hSCAPs

Figure 3 hSCAPs’ odontoblastic differentiation ability and FTO expression under different conditions. (a–c) ALP staining at 3 and 7 days showed decreased ALP activity, and ARS staining at day 14 indicated reduced mineralized nodules in the OM+LPS group compared to the OM group. (d) qRT-PCR revealed elevated mRNA levels of DSPP, DMP1, and COL1 in the OM group while showing decreased levels in the OM+LPS group. (e,f) Western blotting showed increased protein expression of DSPP, DMP1, and COL1 in the OM group, contrasting with decreased expression in the OM+LPS group. (g–i) qRT-PCR and Western blotting showed elevated FTO expression in OM, with reduced expression observed in OM+LPS. Control, growth medium (GM); LPS, GM with 1 µg/mL LPS; OM, odontoblastic induction medium; OM+LPS, OM with 1 µg/mL LPS; hSCAPs, human stem cells from the apical papilla; FTO, fat mass and obesity-associated protein; LPS, lipopolysaccharide; ALP, alkaline phosphatase; ARS, alizarin red S; qRT-PCR, Quantitative Real-Time Polymerase Chain Reaction; DSPP, dentin sialophosphoprotein; DMP1, dentin matrix protein-1; COL1, collagen I.

Application: IF/ICC    Species: Human    Sample: hSCAPs

Figure 3 hSCAPs’ odontoblastic differentiation ability and FTO expression under different conditions. (a–c) ALP staining at 3 and 7 days showed decreased ALP activity, and ARS staining at day 14 indicated reduced mineralized nodules in the OM+LPS group compared to the OM group. (d) qRT-PCR revealed elevated mRNA levels of DSPP, DMP1, and COL1 in the OM group while showing decreased levels in the OM+LPS group. (e,f) Western blotting showed increased protein expression of DSPP, DMP1, and COL1 in the OM group, contrasting with decreased expression in the OM+LPS group. (g–i) qRT-PCR and Western blotting showed elevated FTO expression in OM, with reduced expression observed in OM+LPS. Control, growth medium (GM); LPS, GM with 1 µg/mL LPS; OM, odontoblastic induction medium; OM+LPS, OM with 1 µg/mL LPS; hSCAPs, human stem cells from the apical papilla; FTO, fat mass and obesity-associated protein; LPS, lipopolysaccharide; ALP, alkaline phosphatase; ARS, alizarin red S; qRT-PCR, Quantitative Real-Time Polymerase Chain Reaction; DSPP, dentin sialophosphoprotein; DMP1, dentin matrix protein-1; COL1, collagen I.
4). LncRNA CALB2 sponges miR-30b-3p to promote odontoblast differentiation of human dental pulp stem cells via up-regulating RUNX2. CELLULAR SIGNALLING, 2020 (PubMed: 32565162) [IF=4.4]

Application: WB    Species: human    Sample: hDPSCs

Figure 2. |LncRNA CALB2 promotes odontoblast differentiation of hDPSCs.(E) Protein levels of DSPP and DMP-1 were detected by western blot in indicated groups.
5). Periostin Is a Candidate Regulator of the Host Microenvironment in Regeneration of Pulp and Dentin Complex and Periodontal Ligament in Transplantation with Stem Cell-Conditioned Medium. Stem cells international, 2024 (PubMed: 38435089) [IF=3.8]

Application: WB    Species: Human    Sample:

Figure 1 Expression of DSPP, nestin, DMP1, Runx2, and periostin by dentin induction of dental pulp stem cells. Immunohistochemical staining images after the start of dentin induction 21 days. (Bule: Hoechst 33342 and Green: (a) DSPP, (b) nestin, (c) DMP1, (d) Runx2, and (e) periostin).
6). Embryonic inhibition of colony-stimulating factor 1 receptor induces enlarged cartilaginous zone of the midpalatal suture in postnatal mice. Orthodontics & craniofacial research, 2024 (PubMed: 37904627) [IF=2.4]
7). Role of LINC01133 in Osteogenic Differentiation of Dental Pulp Stem Cells by Targeting miR-199b-5p. Oral health & preventive dentistry, 2023 (PubMed: 35481341) [IF=1.4]

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