MTF1 Antibody | Affinity Biosciences

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MTF1 Antibody - Western blot analysis of MTF1 using Jurkat whole cell lysates.

Product:	MTF1 Antibody
Catalog:	DF8827
Description:	Rabbit polyclonal antibody to MTF1
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	81 kDa; 81kD(Calculated).
Uniprot:	Q14872
RRID:	AB_2842024

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

MTF1 Antibody detects endogenous levels of total MTF1.

RRID:

AB_2842024
Cite Format: Affinity Biosciences Cat# DF8827, RRID:AB_2842024.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Metal regulatory transcription factor 1; Metal response element binding transcription factor 1; Metal responsive transcription factor 1; MRE binding transcription factor; MRE-binding transcription factor 1; MRE-binding transcription factor; MTF 1; MTF1; MTF1_HUMAN; Transcription factor MTF-1; Transcription factor MTF1; Zinc regulatory factor; ZRF;

Immunogens

Immunogen:

Uniprot:

Q14872(MTF1_HUMAN) >>Visit HPA database.

Gene(ID):

MTF1(4520)

Sequence:

Q14872 MTF1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGEHSPDNNIIYFEAEEDELTPDDKMLRFVDKNGLVPSSSGTVYDRTTVLIEQDPGTLEDEDDDGQCGEHLPFLVGGEEGFHLIDHEAMSQGYVQHIISPDQIHLTINPGSTPMPRNIEGATLTLQSECPETKRKEVKRYQCTFEGCPRTYSTAGNLRTHQKTHRGEYTFVCNQEGCGKAFLTSYSLRIHVRVHTKEKPFECDVQGCEKAFNTLYRLKAHQRLHTGKTFNCESEGCSKYFTTLSDLRKHIRTHTGEKPFRCDHDGCGKAFAASHHLKTHVRTHTGERPFFCPSNGCEKTFSTQYSLKSHMKGHDNKGHSYNALPQHNGSEDTNHSLCLSDLSLLSTDSELRENSSTTQGQDLSTISPAIIFESMFQNSDDTAIQEDPQQTASLTESFNGDAESVSDVPPSTGNSASLSLPLVLQPGLSEPPQPLLPASAPSAPPPAPSLGPGSQQAAFGNPPALLQPPEVPVPHSTQFAANHQEFLPHPQAPQPIVPGLSVVAGASASAAAVASAVAAPAPPQSTTEPLPAMVQTLPLGANSVLTNNPTITITPTPNTAILQSSLVMGEQNLQWILNGATSSPQNQEQIQQASKVEKVFFTTAVPVASSPGSSVQQIGLSVPVIIIKQEEACQCQCACRDSAKERASSRRKGCSSPPPPEPSPQAPDGPSLQLPAQTFSSAPVPGSSSSTLPSSCEQSRQAETPSDPQTETLSAMDVSEFLSLQSLDTPSNLIPIEALLQGEEEMGLTSSFSK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14872 As Substrate

Q14872

Site	PTM Type	Source
S5	Phosphorylation	Uniprot
S39	Phosphorylation	Uniprot
K133	Ubiquitination	Uniprot
Y140	Phosphorylation	Uniprot
S152	Phosphorylation	Uniprot
K227	Acetylation	Uniprot
T252	Phosphorylation	Uniprot
T254	Phosphorylation	Uniprot
K257	Ubiquitination	Uniprot
K277	Ubiquitination	Uniprot
T284	Phosphorylation	Uniprot
S293	Phosphorylation	Uniprot
S305	Phosphorylation	Uniprot
K307	Methylation	Uniprot
S620	Phosphorylation	Uniprot

Research Backgrounds

Q14872_MTF1_HUMAN

Function:

Activates the metallothionein I promoter. Binds to the metal responsive element (MRE).

Subcellular Location:

Nucleus.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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MTF1 Antibody - #DF8827