SART3 Antibody - #DF8829
| Product: | SART3 Antibody |
| Catalog: | DF8829 |
| Description: | Rabbit polyclonal antibody to SART3 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 110kDa; 110kD(Calculated). |
| Uniprot: | Q15020 |
| RRID: | AB_2842026 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8829, RRID:AB_2842026.
Fold/Unfold
DSAP1; hSART 3; hSART-3; P100; P110; p110(nrb); RP11 13G14; SART 3; SART-3; SART3; SART3_HUMAN; Squamous cell carcinoma antigen recognized by T cells 3; Squamous cell carcinoma antigen recognized by T-cells 3; Tat interacting protein of 110 kDa; Tat-interacting protein of 110 kDa; Tip110;
Immunogens
- Q15020 SART3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATAAETSASEPEAESKAGPKADGEEDEVKAARTRRKVLSRAVAAATYKTMGPAWDQQEEGVSESDGDEYAMASSAESSPGEYEWEYDEEEEKNQLEIERLEEQLSINVYDYNCHVDLIRLLRLEGELTKVRMARQKMSEIFPLTEELWLEWLHDEISMAQDGLDREHVYDLFEKAVKDYICPNIWLEYGQYSVGGIGQKGGLEKVRSVFERALSSVGLHMTKGLALWEAYREFESAIVEAARLEKVHSLFRRQLAIPLYDMEATFAEYEEWSEDPIPESVIQNYNKALQQLEKYKPYEEALLQAEAPRLAEYQAYIDFEMKIGDPARIQLIFERALVENCLVPDLWIRYSQYLDRQLKVKDLVLSVHNRAIRNCPWTVALWSRYLLAMERHGVDHQVISVTFEKALNAGFIQATDYVEIWQAYLDYLRRRVDFKQDSSKELEELRAAFTRALEYLKQEVEERFNESGDPSCVIMQNWARIEARLCNNMQKARELWDSIMTRGNAKYANMWLEYYNLERAHGDTQHCRKALHRAVQCTSDYPEHVCEVLLTMERTEGSLEDWDIAVQKTETRLARVNEQRMKAAEKEAALVQQEEEKAEQRKRARAEKKALKKKKKIRGPEKRGADEDDEKEWGDDEEEQPSKRRRVENSIPAAGETQNVEVAAGPAGKCAAVDVEPPSKQKEKAASLKRDMPKVLHDSSKDSITVFVSNLPYSMQEPDTKLRPLFEACGEVVQIRPIFSNRGDFRGYCYVEFKEEKSALQALEMDRKSVEGRPMFVSPCVDKSKNPDFKVFRYSTSLEKHKLFISGLPFSCTKEELEEICKAHGTVKDLRLVTNRAGKPKGLAYVEYENESQASQAVMKMDGMTIKENIIKVAISNPPQRKVPEKPETRKAPGGPMLLPQTYGARGKGRTQLSLLPRALQRPSAAAPQAENGPAAAPAVAAPAATEAPKMSNADFAKLFLRK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15020 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T7 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| Y350 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| Y353 | Phosphorylation | Uniprot | |
| R356 | Methylation | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| R446 | Methylation | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| R502 | Methylation | Uniprot | |
| K506 | Acetylation | Uniprot | |
| Y541 | Phosphorylation | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| T657 | Phosphorylation | Uniprot | |
| C670 | S-Nitrosylation | Uniprot | |
| K694 | Ubiquitination | Uniprot | |
| S740 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S778 | Phosphorylation | Uniprot | |
| S795 | Phosphorylation | Uniprot | |
| K822 | Ubiquitination | Uniprot | |
| R831 | Methylation | Uniprot | |
| T834 | Phosphorylation | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| K867 | Ubiquitination | Uniprot | |
| T889 | Phosphorylation | Uniprot | |
| R906 | Methylation | Uniprot | |
| K908 | Methylation | Uniprot | |
| T911 | Phosphorylation | Uniprot | |
| R918 | Methylation | Uniprot | |
| S924 | Phosphorylation | Uniprot | |
| K958 | Ubiquitination | Uniprot |
Research Backgrounds
U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation. Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites. May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly. May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair. May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC (By similarity).
Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication.
Nucleus>Nucleoplasm. Nucleus>Cajal body. Nucleus speckle. Cytoplasm.
Ubiquitously expressed.
Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with AGO1 and AGO2. Interacts with PRPF3 and USP4; the interaction with PRPF3 is direct and recruits USP4 to its substrate PRPF3. Interacts with USP15; the interaction is direct. Interacts with HIV-1 Tat.
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