LRRK1 Antibody - #DF8834
(4)
Product Info
Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Horse(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
LRRK1 Antibody detects endogenous levels of total LRRK1.
RRID:
AB_2842031
Cite Format: Affinity Biosciences Cat# DF8834, RRID:AB_2842031.
Cite Format: Affinity Biosciences Cat# DF8834, RRID:AB_2842031.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
KIAA1790; Leucine rich repeat serine/threonine protein kinase 1; Leucine-rich repeat serine/threonine-protein kinase 1; LRRK 1; Lrrk1; LRRK1_HUMAN; RIPK6; Roco1;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q38SD2 LRRK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGMSQRPPSMYWCVGPEESAVCPERAMETLNGAGDTGGKPSTRGGDPAARSRRTEGIRAAYRRGDRGGARDLLEEACDQCASQLEKGQLLSIPAAYGDLEMVRYLLSKRLVELPTEPTDDNPAVVAAYFGHTAVVQELLESLPGPCSPQRLLNWMLALACQRGHLGVVKLLVLTHGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLLRHGAYFCSYILLDSPDPSKHLLRKYFIEASPLPSSYPGKTALRVKWSHLRLPWVDLDWLIDISCQITELDLSANCLATLPSVIPWGLINLRKLNLSDNHLGELPGVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNCLEKLFEEENATNWIGLRKLQELDISDNKLTELPALFLHSFKSLNSLNVSRNNLKVFPDPWACPLKCCKASRNALECLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLRLQGNQLAALPPQEKWTCRQLKTLDLSRNQLGKNEDGLKTKRIAFFTTRGRQRSGTEAASVLEFPAFLSESLEVLCLNDNHLDTVPPSVCLLKSLSELYLGNNPGLRELPPELGQLGNLWQLDTEDLTISNVPAEIQKEGPKAMLSYLRAQLRKAEKCKLMKMIIVGPPRQGKSTLLEILQTGRAPQVVHGEATIRTTKWELQRPAGSRAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKSLEGQEGLRQLIFHVTCSMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSRDDDVQYLTDRQLEQLVEQTPDNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGMKIVCQSEVRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCETAWAQHTDPSEKSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDEGSVLGQGGSGTVIYRARYQGQPVAVKRFHIKKFKNFANVPADTMLRHLRATDAMKNFSEFRQEASMLHALQHPCIVALIGISIHPLCFALELAPLSSLNTVLSENARDSSFIPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDVKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIAKKLSKGIRPVLGQPEEVQFRRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYELCCGKQTAFFSSQGQEYTVVFWDGKEESRNYTVVNTEKGLMEVQRMCCPGMKVSCQLQVQRSLWTATEDQKIYIYTLKGMCPLNTPQQALDTPAVVTCFLAVPVIKKNSYLVLAGLADGLVAVFPVVRGTPKDSCSYLCSHTANRSKFSIADEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCASLEICRRLEPYMAPSMVTSVVCSSEGRGEEVVWCLDDKANSLVMYHSTTYQLCARYFCGVPSPLRDMFPVRPLDTEPPAASHTANPKVPEGDSIADVSIMYSEELGTQILIHQESLTDYCSMSSYSSSPPRQAARSPSSLPSSPASSSSVPFSTDCEDSDMLHTPGAASDRSEHDLTPMDGETFSQHLQAVKILAVRDLIWVPRRGGDVIVIGLEKDSGAQRGRVIAVLKARELTPHGVLVDAAVVAKDTVVCTFENENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Xenopus
83
Pig
0
Bovine
0
Sheep
0
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q38SD2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T30 | Phosphorylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| T358 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| Y971 | Phosphorylation | P00533 (EGFR) | Uniprot |
| K1054 | Methylation | Uniprot | |
| Y1063 | Phosphorylation | Uniprot | |
| S1064 | Phosphorylation | Uniprot | |
| Y1098 | Phosphorylation | Uniprot | |
| S1100 | Phosphorylation | Uniprot | |
| S1103 | Phosphorylation | Uniprot | |
| S1104 | Phosphorylation | Uniprot | |
| S1241 | Phosphorylation | Uniprot | |
| S1246 | Phosphorylation | Uniprot | |
| K1276 | Acetylation | Uniprot | |
| K1278 | Acetylation | Uniprot | |
| T1295 | Phosphorylation | Uniprot | |
| S1302 | Phosphorylation | Uniprot | |
| T1427 | Phosphorylation | P06493 (CDK1) | Uniprot |
| Y1430 | Phosphorylation | Uniprot | |
| T1628 | Phosphorylation | Uniprot | |
| K1678 | Ubiquitination | Uniprot | |
| S1817 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S1855 | Phosphorylation | Uniprot | |
| S1858 | Phosphorylation | Uniprot |
PTMs - Q38SD2 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| Q96SN8 (CDK5RAP2) | T102 | Uniprot |
| Q96SN8 (CDK5RAP2) | S140 | Uniprot |
Research Backgrounds
Subcellular Location:
Cytoplasm.
Subunit Structure:
Homodimer.
Family&Domains:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ROCO subfamily.
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