TENS3 Antibody - #DF8838

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Product: TENS3 Antibody
Catalog: DF8838
Description: Rabbit polyclonal antibody to TENS3
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 150~180kD; 155kD(Calculated).
Uniprot: Q68CZ2
RRID: AB_2842035

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(88%), Bovine(88%), Horse(88%), Sheep(88%)
Clonality:
Polyclonal
Specificity:
TENS3 Antibody detects endogenous levels of total TENS3.
RRID:
AB_2842035
Cite Format: Affinity Biosciences Cat# DF8838, RRID:AB_2842035.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

TEM 6; TEM6; TENS 1; TENS 3; TENS1; TENS3; TENS3_HUMAN; Tensin-3; tensin-like SH2 domain containing 1; Tensin-like SH2 domain containing protein 1; Tensin-like SH2 domain- containing protein 1; Tensin-like SH2 domain-containing protein 1; thyroid specific PTB domain protein; TNS 3; TNS3; TPP; Tumor endothelial marker 6;

Immunogens

Immunogen:
Uniprot:

Q68CZ2(TENS3_HUMAN) >>Visit HPA database.

Gene(ID):
TNS3(64759)
Expression:
Q68CZ2 TENS3_HUMAN:

Expressed in umbilical vein endothelial cells, epithelial cells, and fibroblasts cells (at protein level). Highly expressed in thyroid, kidney and placenta. Low expression in heart, skeletal muscle, spleen, liver, and lung. Expressed in tumor endothelial cells. Expression seems to be down-regulated in thyroid tumor tissues and in anaplastic carcinomas.

Sequence:
MEEGHGLDLTYITERIIAVSFPAGCSEESYLHNLQEVTRMLKSKHGDNYLVLNLSEKRYDLTKLNPKIMDVGWPELHAPPLDKMCTICKAQESWLNSNLQHVVVIHCRGGKGRIGVVISSYMHFTNVSASADQALDRFAMKKFYDDKVSALMQPSQKRYVQFLSGLLSGSVKMNASPLFLHFVILHGTPNFDTGGVCRPFLKLYQAMQPVYTSGIYNVGPENPSRICIVIEPAQLLKGDVMVKCYHKKYRSATRDVIFRLQFHTGAVQGYGLVFGKEDLDNASKDDRFPDYGKVELVFSATPEKIQGSEHLYNDHGVIVDYNTTDPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSSSDPGIPGGPQAIPATNSPDHSDHTLSVSSDSGHSTASARTDKTEERLAPGTRRGLSAQEKAELDQLLSGFGLEDPGSSLKEMTDARSKYSGTRHVVPAQVHVNGDAALKDRETDILDDEMPHHDLHSVDSLGTLSSSEGPQSAHLGPFTCHKSSQNSLLSDGFGSNVGEDPQGTLVPDLGLGMDGPYERERTFGSREPKQPQPLLRKPSVSAQMQAYGQSSYSTQTWVRQQQMVVAHQYSFAPDGEARLVSRCPADNPGLVQAQPRVPLTPTRGTSSRVAVQRGVGSGPHPPDTQQPSPSKAFKPRFPGDQVVNGAGPELSTGPSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHMNALGSQANGSVSPDSVGGGLRASSRLPDTGEGPSRATGRQGSSAEQPLGGRLRKLSLGQYDNDAGGQLPFSKCAWGKAGVDYAPNLPPFPSPADVKETMTPGYPQDLDIIDGRILSSKESMCSTPAFPVSPETPYVKTALRHPPFSPPEPPLSSPASQHKGGREPRSCPETLTHAVGMSESPIGPKSTMLRADASSTPSFQQAFASSCTISSNGPGQRRESSSSAERQWVESSPKPMVSLLGSGRPTGSPLSAEFSGTRKDSPVLSCFPPSELQAPFHSHELSLAEPPDSLAPPSSQAFLGFGTAPVGSGLPPEEDLGALLANSHGASPTPSIPLTATGAADNGFLSHNFLTVAPGHSSHHSPGLQGQGVTLPGQPPLPEKKRASEGDRSLGSVSPSSSGFSSPHSGSTISIPFPNVLPDFSKASEAASPLPDSPGDKLVIVKFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQLNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLIPERDPLEEIAESSPQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSITLVQEPPPVSTVVHFKVSAQGITLTDNQRKLFFRRHYPVNSVIFCALDPQDRKWIKDGPSSKVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIGSPKKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q68CZ2 As Substrate

Site PTM Type Enzyme
K44 Acetylation
K57 Ubiquitination
K63 Ubiquitination
K67 Ubiquitination
K142 Ubiquitination
K147 Ubiquitination
K157 Ubiquitination
Y204 Phosphorylation
K237 Ubiquitination
K243 Ubiquitination
K284 Ubiquitination
S299 Phosphorylation
Y321 Phosphorylation
T324 Phosphorylation
S332 Phosphorylation
Y333 Phosphorylation
S337 Phosphorylation
T345 Phosphorylation
S352 Phosphorylation
Y354 Phosphorylation
S361 Phosphorylation
S383 Phosphorylation
T405 Phosphorylation
T413 Phosphorylation
S418 Phosphorylation
K422 Ubiquitination
S440 Phosphorylation
K442 Ubiquitination
Y451 Phosphorylation
K471 Ubiquitination
S492 Phosphorylation
S516 Phosphorylation
S519 Phosphorylation
Y549 Phosphorylation
K561 Ubiquitination
K569 Ubiquitination
S571 Phosphorylation
S573 Phosphorylation
Y579 Phosphorylation
S582 Phosphorylation
S583 Phosphorylation
Y584 Phosphorylation
S585 Phosphorylation
T586 Phosphorylation
T588 Phosphorylation
Y601 Phosphorylation
S602 Phosphorylation
R628 Methylation
T632 Phosphorylation
T637 Phosphorylation
S638 Phosphorylation
R640 Methylation
R645 Methylation
S649 Phosphorylation
S660 Phosphorylation
S662 Phosphorylation
K663 Ubiquitination
S683 Phosphorylation
T684 Phosphorylation
S687 Phosphorylation
S690 Phosphorylation
T692 Phosphorylation
S698 Phosphorylation
S725 Phosphorylation
S732 Phosphorylation
S735 Phosphorylation
T749 Phosphorylation
S762 Phosphorylation
S763 Phosphorylation
R771 Methylation
K774 Ubiquitination
S776 Phosphorylation
Y780 Phosphorylation
K797 Methylation
Y802 Phosphorylation
S811 Phosphorylation
K816 Ubiquitination
T818 Phosphorylation
T820 Phosphorylation
Y823 Phosphorylation
S836 Phosphorylation
S837 Phosphorylation
K838 Ubiquitination
S840 Phosphorylation
S843 Phosphorylation
T844 Phosphorylation
S850 Phosphorylation
T853 Phosphorylation
Y855 Phosphorylation
T858 Phosphorylation
S866 Phosphorylation
S873 Phosphorylation
S874 Phosphorylation
S877 Phosphorylation
S887 Phosphorylation
S899 Phosphorylation
S901 Phosphorylation
T917 Phosphorylation
S919 Phosphorylation
S926 Phosphorylation
S941 Phosphorylation
S942 Phosphorylation
S943 Phosphorylation
S944 Phosphorylation
S952 Phosphorylation
S953 Phosphorylation
S959 Phosphorylation
S963 Phosphorylation
T967 Phosphorylation
S969 Phosphorylation
S972 Phosphorylation
S976 Phosphorylation
S982 Phosphorylation
S1113 Phosphorylation
S1115 Phosphorylation
S1118 Phosphorylation
S1119 Phosphorylation
S1122 Phosphorylation
S1123 Phosphorylation
S1126 Phosphorylation
S1128 Phosphorylation
T1129 Phosphorylation
S1131 Phosphorylation
K1143 Ubiquitination
S1145 Phosphorylation
S1149 Phosphorylation
S1154 Phosphorylation
K1158 Ubiquitination
K1163 Ubiquitination
K1170 Ubiquitination
Y1173 Phosphorylation P12931 (SRC)
K1174 Ubiquitination
K1187 Ubiquitination
K1189 Ubiquitination
S1193 Phosphorylation
S1199 Phosphorylation
S1201 Phosphorylation
Y1206 Phosphorylation P12931 (SRC)
T1214 Phosphorylation
S1218 Phosphorylation
K1224 Ubiquitination
K1225 Ubiquitination
K1244 Ubiquitination
K1249 Ubiquitination
S1252 Phosphorylation
Y1256 Phosphorylation P12931 (SRC)
S1259 Phosphorylation
T1261 Phosphorylation
S1293 Phosphorylation
T1337 Phosphorylation
K1392 Ubiquitination
K1398 Ubiquitination
K1406 Ubiquitination
S1441 Phosphorylation

Research Backgrounds

Function:

May play a role in actin remodeling. Involved in the dissociation of the integrin-tensin-actin complex. EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1. Seems to be involved in mammary cell migration. May be involved in cell migration and bone development (By similarity).

PTMs:

EGF/epidermal growth factor induces tyrosine phosphorylation in a time- and dose-dependent manner.

Subcellular Location:

Cell junction>Focal adhesion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in umbilical vein endothelial cells, epithelial cells, and fibroblasts cells (at protein level). Highly expressed in thyroid, kidney and placenta. Low expression in heart, skeletal muscle, spleen, liver, and lung. Expressed in tumor endothelial cells. Expression seems to be down-regulated in thyroid tumor tissues and in anaplastic carcinomas.

Subunit Structure:

EGF promotes the interaction with EGFR. Interacts with PTK2/FAK1 and BCAR1. Tyrosine phosphorylation is critical for these interactions.

Family&Domains:

Belongs to the PTEN phosphatase protein family.

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