GSG2 Antibody - #DF8849

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Product: GSG2 Antibody
Catalog: DF8849
Description: Rabbit polyclonal antibody to GSG2
Application: WB
Reactivity: Human
Mol.Wt.: 88 kDa; 88kD(Calculated).
Uniprot: Q8TF76
RRID: AB_2842046

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
GSG2 Antibody detects endogenous levels of total GSG2.
RRID:
AB_2842046
Cite Format: Affinity Biosciences Cat# DF8849, RRID:AB_2842046.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

germ cell associated 2; Germ cell-specific gene 2 protein; GSG2; H-haspin; Haploid germ cell-specific nuclear protein kinase; HASP_HUMAN; Serine/threonine-protein kinase haspin;

Immunogens

Immunogen:
Uniprot:

Q8TF76(HASP_HUMAN) >>Visit HPA database.

Gene(ID):
GSG2(83903)
Expression:
Q8TF76 HASP_HUMAN:

Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.

Sequence:
MAASLPGPGSRLFRTYGAADGRRQRRPGREAAQWFPPQDRRRFFNSSGSSDASIGDPSQSDDPDDPDDPDFPGSPVRRRRRRPGGRVPKDRPSLTVTPKRWKLRARPSLTVTPRRLGLRARPPQKCSTPCGPLRLPPFPSRDSGRLSPDLSVCGQPRDGDELGISASLFSSLASPCPGSPTPRDSVISIGTSACLVAASAVPSGLHLPEVSLDRASLPCSQEEATGGAKDTRMVHQTRASLRSVLFGLMNSGTPEDSEFRADGKNMRESCCKRKLVVGNGPEGPGLSSTGKRRATGQDSCQERGLQEAVRREHQEASVPKGRIVPRGIDRLERTRSSRKSKHQEATETSLLHSHRFKKGQKLGKDSFPTQDLTPLQNVCFWTKTRASFSFHKKKIVTDVSEVCSIYTTATSLSGSLLSECSNRPVMNRTSGAPSSWHSSSMYLLSPLNTLSISNKKASDAEKVYGECSQKGPVPFSHCLPTEKLQRCEKIGEGVFGEVFQTIADHTPVAIKIIAIEGPDLVNGSHQKTFEEILPEIIISKELSLLSGEVCNRTEGFIGLNSVHCVQGSYPPLLLKAWDHYNSTKGSANDRPDFFKDDQLFIVLEFEFGGIDLEQMRTKLSSLATAKSILHQLTASLAVAEASLRFEHRDLHWGNVLLKKTSLKKLHYTLNGKSSTIPSCGLQVSIIDYTLSRLERDGIVVFCDVSMDEDLFTGDGDYQFDIYRLMKKENNNRWGEYHPYSNVLWLHYLTDKMLKQMTFKTKCNTPAMKQIKRKIQEFHRTMLNFSSATDLLCQHSLFK

PTMs - Q8TF76 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
S58 Phosphorylation
S93 Phosphorylation Q96GD4 (AURKB) , PR:Q96GD4 (hAURKB)
T97 Phosphorylation
K99 Ubiquitination
S108 Phosphorylation PR:Q96GD4 (hAURKB)
T110 Phosphorylation
T112 Phosphorylation
T128 Phosphorylation P06493 (CDK1)
S140 Phosphorylation
S143 Phosphorylation PR:Q96GD4 (hAURKB) , Q96GD4 (AURKB)
S147 Phosphorylation
S151 Phosphorylation
T191 Phosphorylation
S216 Phosphorylation
S220 Phosphorylation
K229 Ubiquitination
T231 Phosphorylation
S240 Phosphorylation
S251 Phosphorylation
T253 Phosphorylation
K274 Ubiquitination
S287 Phosphorylation
S288 Phosphorylation
T289 Phosphorylation
K291 Ubiquitination
S340 Phosphorylation
K341 Ubiquitination
S366 Phosphorylation
T373 Phosphorylation
S387 Phosphorylation
S389 Phosphorylation
K462 Ubiquitination
K470 Ubiquitination
K483 Ubiquitination
K584 Ubiquitination
K618 Ubiquitination
K664 Ubiquitination
K759 Methylation
T764 Phosphorylation
K768 Ubiquitination

Research Backgrounds

Function:

Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.

PTMs:

Autophosphorylated on both serine and threonine residues (By similarity). Strongly phosphorylated during mitosis but this does not appear to significantly affect its intrinsic kinase activity. Phosphorylation by AURKB is required for full activity toward histone H3 at 'Ser-3' in mitosis.

Subcellular Location:

Nucleus. Chromosome. Cytoplasm>Cytoskeleton>Spindle.
Note: Nuclear during interphase and associates with the chromosomes and spindle apparatus during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.

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