GTF2H4 Antibody - #DF8857

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Product: GTF2H4 Antibody
Catalog: DF8857
Description: Rabbit polyclonal antibody to GTF2H4
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Dog, Xenopus
Mol.Wt.: 52 kDa; 52kD(Calculated).
Uniprot: Q92759
RRID: AB_2842054

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 100ul $280 In stock
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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GTF2H4 Antibody detects endogenous levels of total GTF2H4.
RRID:
AB_2842054
Cite Format: Affinity Biosciences Cat# DF8857, RRID:AB_2842054.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Basic transcription factor 2 52 kDa subunit; Basic transcription factor 52 kDa subunit; BTF2 p52; General transcription factor IIH polypeptide 4 52kDa; General transcription factor IIH polypeptide 4 52kDa subunit; General transcription factor IIH polypeptide 4 52kDa variant; General transcription factor IIH polypeptide 4; General transcription factor IIH subunit 4; Gtf2h4; OTTHUMP00000029049; OTTHUMP00000164874; P52; TF2H4_HUMAN; TFB2; TFIIH basal transcription factor complex p52 subunit; TFIIH protein; TIFIIH; Transcription factor II H; Transcription factor IIH 52 kDa subunit;

Immunogens

Immunogen:

A synthesized peptide derived from human GTF2H4, corresponding to a region within the internal amino acids.

Uniprot:

Q92759(TF2H4_HUMAN) >>Visit HPA database.

Gene(ID):
GTF2H4(2968)
Sequence:
MESTPSRGLNRVHLQCRNLQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQGLILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPSLDKYAEERWEVVLHFMVGSPSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVSGAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
63
Pig
0
Horse
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the TFB2 family.

Research Fields

· Genetic Information Processing > Transcription > Basal transcription factors.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

References

1). Anti-hsa-miR-59 alleviates premature senescence associated with Hutchinson-Gilford progeria syndrome in mice. The EMBO journal, 2023 (PubMed: 36382717) [IF=9.4]

Application: WB    Species: human    Sample: HGPS cells

Figure 4. Analysis of the effect of HMGA1 on the combination of RNAPII and TFIIH in HGPS cells A. Interaction of endogenous HMGA1 with HMGA2 and TFIIH4 complex was analyzed by Co‐IP assay using anti‐HMGA1 antibody in H1299 cells. Pulled‐down protein complexes were analyzed. Benzonase (B) was added in cell lysate. B. Interaction of endogenous HMGA2 with HMGA1 and TFIIH4 complex was analyzed by Co‐IP assay using anti‐HMGA2 antibody in H1299 cells. Pulled‐down protein complexes were analyzed. C. HMGA1‐associated proteins from HEK293T cells expressing FLAG‐HMGA1 were IP with anti‐Flag antibody. The protein bands were analyzed by mass spectrometry. Representative peptide fragments of HMGA1 associated proteins and peptide coverage of the indicated proteins were shown. D. Interaction of endogenous CDK7 with HMGAs, XPB and TFIIH4 was analyzed by Co‐IP assay using anti‐CDK7 antibody in H1299 cells. Pulled‐down protein complexes were analyzed. E‐F. HEK293T cells were transfected with Flag‐HMGA1 (E) or CDK7 (F). HMGA1 or CDK7 was IP with anti‐Flag antibody and anti‐CDK7 antibody. G. HEK293T cell lysates were incubated with GST or GST‐HMGA1 sepharose beads. Pulled‐down protein complexes were analyzed. H. Scheme of HMGA1 wild‐type structure, including AT hooks (AT) and acidic tail (C tail), and deletion mutants used for immunoprecipitation experiments below. I. HEK293T cells were transfected with Flag‐HMGA1 deletion mutants. IP of Flag was performed. Pulled‐down protein complexes were analyzed. J. HEK293T cell lysates were incubated with GST or GST‐HMGA1 deletion mutants sepharose beads. Recombinant proteins from pull‐down assays visualized by silver staining and Western blot. K, L. Progerin‐expressing H1299 cells were transfected with His‐control or His‐HMGA1. Immunoprecipitation of RNA Pol II (K) or CDK7 (L) with anti‐RNAPII antibody or anti‐CDK7 antibody was performed, respectively. Pulled‐down protein complexes were analyzed. M, N. RNAPII, the RNAPII Ser2P, and RNAPII Ser5P in HGAFDFN003 cells (M) and 88, 92‐year‐old cells (N) compared with CRL‐1474 cells were detected by Western blot. O. Progerin‐expressing CRL‐1474 cells were transfected with His‐control or His‐HMGA1. Indicated proteins and modifications were detected by western blot. P. Infection with siCtrl or siCDK7 in Flag‐HMGA1‐transfected HGPS cells (HGAFDFN003), cyclin A1, RNAPII, the RNAPII Ser2P and RNAPII Ser5P was detected. Q. Infection with control shRNA (shCtrl) or HMGA1 shRNA (shHMGA1) in anti‐miR‐59‐transfected HGAFDFN003 cells. Indicated proteins and modifications were detected by western blot. Source data are available online for this figure.

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