PINX1 Antibody | Affinity Biosciences

1/1

PINX1 Antibody - Western blot analysis of PINX1 using NIH-3T3 whole cell lysates.

Product:	PINX1 Antibody
Catalog:	DF8858
Description:	Rabbit polyclonal antibody to PINX1
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Rabbit
Mol.Wt.:	37 kDa; 37kD(Calculated).
Uniprot:	Q96BK5
RRID:	AB_2842055

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(83%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

PINX1 Antibody detects endogenous levels of total PINX1.

RRID:

AB_2842055
Cite Format: Affinity Biosciences Cat# DF8858, RRID:AB_2842055.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

67-11-3 protein; FLJ20565; Hepatocellular carcinoma-related putative tumor suppressor; Liver-related putative tumor suppressor; LPTL; LPTS; MGC8850; OTTHUMP00000224984; OTTHUMP00000224985; OTTHUMP00000224986; OTTHUMP00000225053; OTTHUMP00000225054; PIN2 interacting protein 1; Pin2-interacting protein X1; PIN2/TERF1-interacting telomerase inhibitor 1; PINX1; PINX1_HUMAN; Protein 67-11-3; TRF1-interacting protein 1;

Immunogens

Immunogen:

Uniprot:

Q96BK5(PINX1_HUMAN) >>Visit HPA database.

Gene(ID):

PINX1(54984)

Expression:

Q96BK5 PINX1_HUMAN:

Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.

Sequence:

Q96BK5 PINX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSMLAERRRKQKWAVDPQNTAWSNDDSKFGQRMLEKMGWSKGKGLGAQEQGATDHIKVQVKNNHLGLGATINNEDNWIAHQDDFNQLLAELNTCHGQETTDSSDKKEKKSFSLEEKSKISKNRVHYMKFTKGKDLSSRSKTDLDCIFGKRQSKKTPEGDASPSTPEENETTTTSAFTIQEYFAKRMAALKNKPQVPVPGSDISETQVERKRGKKRNKEATGKDVESYLQPKAKRHTEGKPERAEAQERVAKKKSAPAEEQLRGPCWDQSSKASAQDAGDHVQPPEGRDFTLKPKKRRGKKKLQKPVEIAEDATLEETLVKKKKKKDSK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Sheep

Horse

Bovine

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96BK5 As Substrate

Q96BK5

Site	PTM Type	Enzyme	Source
R7	Methylation		Uniprot
K12	Ubiquitination		Uniprot
S110	Phosphorylation	P53350 (PLK1)	Uniprot
S117	Phosphorylation	P53350 (PLK1)	Uniprot
Y126	Phosphorylation		Uniprot
T141	Phosphorylation	P53350 (PLK1)	Uniprot
S161	Phosphorylation		Uniprot
S163	Phosphorylation		Uniprot
T164	Phosphorylation		Uniprot
T170	Phosphorylation		Uniprot
T171	Phosphorylation		Uniprot
S200	Phosphorylation		Uniprot
S226	Phosphorylation	P53350 (PLK1)	Uniprot
Y227	Phosphorylation		Uniprot
K231	Sumoylation		Uniprot
K252	Methylation		Uniprot
T290	Phosphorylation		Uniprot
T317	Phosphorylation	P53350 (PLK1)	Uniprot

Research Backgrounds

Q96BK5_PINX1_HUMAN

Function:

Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor.

Subcellular Location:

Nucleus. Nucleus>Nucleolus. Chromosome>Telomere. Chromosome>Centromere>Kinetochore.
Note: Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE.

Tissue Specificity:

Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.

Subunit Structure:

Interacts with MCRS1, TERT, TERF1, NCL/nucleolin, and the telomerase RNA.

Family&Domains:

The TID (telomerase inhibiting domain) domain is sufficient to bind TERT and inhibit its activity.

The TBM domain mediates interaction with TERF1.

Belongs to the PINX1 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PINX1 Antibody - #DF8858