AKAP9 Antibody - #DF8864

(3)  
Product: AKAP9 Antibody
Catalog: DF8864
Description: Rabbit polyclonal antibody to AKAP9
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 454kDa; 453kD(Calculated).
Uniprot: Q99996
RRID: AB_2842061

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Sheep(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
AKAP9 Antibody detects endogenous levels of total AKAP9.
RRID:
AB_2842061
Cite Format: Affinity Biosciences Cat# DF8864, RRID:AB_2842061.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A kinase (PRKA) anchor protein (yotiao) 9; A kinase (PRKA) anchor protein 9; A kinase anchor protein 350kDa; A kinase anchor protein 450 kDa; A kinase anchor protein 9; A kinase anchoring protein 350; A kinase anchoring protein 450; A-kinase anchor protein 350 kDa; A-kinase anchor protein 450 kDa; A-kinase anchor protein 9; AKAP 120-like protein; AKAP 350; AKAP 450; AKAP 9; AKAP-9; AKAP120 like protein; AKAP350; AKAP450; AKAP9; AKAP9_HUMAN; Centrosome and Golgi localized PKN associated protein; Centrosome and golgi localized protein; Centrosome- and Golgi-localized PKN-associated protein; CG NAP; CG-NAP; hgAKAP 350; hgAKAP350; Hyperion; KIAA0803; Kinase N associated protein; MU RMS 40.16A; PRKA 9; PRKA9; Protein hyperion; Protein kinase A anchoring protein 9; Protein kinase A-anchoring protein 9; Protein yotiao; Yotiao;

Immunogens

Immunogen:
Uniprot:

Q99996(AKAP9_HUMAN) >>Visit HPA database.

Gene(ID):
AKAP9(10142)
Expression:
Q99996 AKAP9_HUMAN:

Widely expressed (PubMed:10202149). Isoform 4: Highly expressed in skeletal muscle and in pancreas (PubMed:9482789).

Sequence:
MEDEERQKKLEAGKAKLAQFRQRKAQSDGQSPSKKQKKKRKTSSSKHDVSAHHDLNIDQSQCNEMYINSSQRVESTVIPESTIMRTLHSGEITSHEQGFSVELESEISTTADDCSSEVNGCSFVMRTGKPTNLLREEEFGVDDSYSEQGAQDSPTHLEMMESELAGKQHEIEELNRELEEMRVTYGTEGLQQLQEFEAAIKQRDGIITQLTANLQQARREKDETMREFLELTEQSQKLQIQFQQLQASETLRNSTHSSTAADLLQAKQQILTHQQQLEEQDHLLEDYQKKKEDFTMQISFLQEKIKVYEMEQDKKVENSNKEEIQEKETIIEELNTKIIEEEKKTLELKDKLTTADKLLGELQEQIVQKNQEIKNMKLELTNSKQKERQSSEEIKQLMGTVEELQKRNHKDSQFETDIVQRMEQETQRKLEQLRAELDEMYGQQIVQMKQELIRQHMAQMEEMKTRHKGEMENALRSYSNITVNEDQIKLMNVAINELNIKLQDTNSQKEKLKEELGLILEEKCALQRQLEDLVEELSFSREQIQRARQTIAEQESKLNEAHKSLSTVEDLKAEIVSASESRKELELKHEAEVTNYKIKLEMLEKEKNAVLDRMAESQEAELERLRTQLLFSHEEELSKLKEDLEIEHRINIEKLKDNLGIHYKQQIDGLQNEMSQKIETMQFEKDNLITKQNQLILEISKLKDLQQSLVNSKSEEMTLQINELQKEIEILRQEEKEKGTLEQEVQELQLKTELLEKQMKEKENDLQEKFAQLEAENSILKDEKKTLEDMLKIHTPVSQEERLIFLDSIKSKSKDSVWEKEIEILIEENEDLKQQCIQLNEEIEKQRNTFSFAEKNFEVNYQELQEEYACLLKVKDDLEDSKNKQELEYKSKLKALNEELHLQRINPTTVKMKSSVFDEDKTFVAETLEMGEVVEKDTTELMEKLEVTKREKLELSQRLSDLSEQLKQKHGEISFLNEEVKSLKQEKEQVSLRCRELEIIINHNRAENVQSCDTQVSSLLDGVVTMTSRGAEGSVSKVNKSFGEESKIMVEDKVSFENMTVGEESKQEQLILDHLPSVTKESSLRATQPSENDKLQKELNVLKSEQNDLRLQMEAQRICLSLVYSTHVDQVREYMENEKDKALCSLKEELIFAQEEKIKELQKIHQLELQTMKTQETGDEGKPLHLLIGKLQKAVSEECSYFLQTLCSVLGEYYTPALKCEVNAEDKENSGDYISENEDPELQDYRYEVQDFQENMHTLLNKVTEEYNKLLVLQTRLSKIWGQQTDGMKLEFGEENLPKEETEFLSIHSQMTNLEDIDVNHKSKLSSLQDLEKTKLEEQVQELESLISSLQQQLKETEQNYEAEIHCLQKRLQAVSESTVPPSLPVDSVVITESDAQRTMYPGSCVKKNIDGTIEFSGEFGVKEETNIVKLLEKQYQEQLEEEVAKVIVSMSIAFAQQTELSRISGGKENTASSKQAHAVCQQEQHYFNEMKLSQDQIGFQTFETVDVKFKEEFKPLSKELGEHGKEILLSNSDPHDIPESKDCVLTISEEMFSKDKTFIVRQSIHDEISVSSMDASRQLMLNEEQLEDMRQELVRQYQEHQQATELLRQAHMRQMERQREDQEQLQEEIKRLNRQLAQRSSIDNENLVSERERVLLEELEALKQLSLAGREKLCCELRNSSTQTQNGNENQGEVEEQTFKEKELDRKPEDVPPEILSNERYALQKANNRLLKILLEVVKTTAAVEETIGRHVLGILDRSSKSQSSASLIWRSEAEASVKSCVHEEHTRVTDESIPSYSGSDMPRNDINMWSKVTEEGTELSQRLVRSGFAGTEIDPENEELMLNISSRLQAAVEKLLEAISETSSQLEHAKVTQTELMRESFRQKQEATESLKCQEELRERLHEESRAREQLAVELSKAEGVIDGYADEKTLFERQIQEKTDIIDRLEQELLCASNRLQELEAEQQQIQEERELLSRQKEAMKAEAGPVEQQLLQETEKLMKEKLEVQCQAEKVRDDLQKQVKALEIDVEEQVSRFIELEQEKNTELMDLRQQNQALEKQLEKMRKFLDEQAIDREHERDVFQQEIQKLEQQLKVVPRFQPISEHQTREVEQLANHLKEKTDKCSELLLSKEQLQRDIQERNEEIEKLEFRVRELEQALLVSADTFQKVEDRKHFGAVEAKPELSLEVQLQAERDAIDRKEKEITNLEEQLEQFREELENKNEEVQQLHMQLEIQKKESTTRLQELEQENKLFKDDMEKLGLAIKESDAMSTQDQHVLFGKFAQIIQEKEVEIDQLNEQVTKLQQQLKITTDNKVIEEKNELIRDLETQIECLMSDQECVKRNREEEIEQLNEVIEKLQQELANIGQKTSMNAHSLSEEADSLKHQLDVVIAEKLALEQQVETANEEMTFMKNVLKETNFKMNQLTQELFSLKRERESVEKIQSIPENSVNVAIDHLSKDKPELEVVLTEDALKSLENQTYFKSFEENGKGSIINLETRLLQLESTVSAKDLELTQCYKQIKDMQEQGQFETEMLQKKIVNLQKIVEEKVAAALVSQIQLEAVQEYAKFCQDNQTISSEPERTNIQNLNQLREDELGSDISALTLRISELESQVVEMHTSLILEKEQVEIAEKNVLEKEKKLLELQKLLEGNEKKQREKEKKRSPQDVEVLKTTTELFHSNEESGFFNELEALRAESVATKAELASYKEKAEKLQEELLVKETNMTSLQKDLSQVRDHLAEAKEKLSILEKEDETEVQESKKACMFEPLPIKLSKSIASQTDGTLKISSSNQTPQILVKNAGIQINLQSECSSEEVTEIISQFTEKIEKMQELHAAEILDMESRHISETETLKREHYVAVQLLKEECGTLKAVIQCLRSKEGSSIPELAHSDAYQTREICSSDSGSDWGQGIYLTHSQGFDIASEGRGEESESATDSFPKKIKGLLRAVHNEGMQVLSLTESPYSDGEDHSIQQVSEPWLEERKAYINTISSLKDLITKMQLQREAEVYDSSQSHESFSDWRGELLLALQQVFLEERSVLLAAFRTELTALGTTDAVGLLNCLEQRIQEQGVEYQAAMECLQKADRRSLLSEIQALHAQMNGRKITLKREQESEKPSQELLEYNIQQKQSQMLEMQVELSSMKDRATELQEQLSSEKMVVAELKSELAQTKLELETTLKAQHKHLKELEAFRLEVKDKTDEVHLLNDTLASEQKKSRELQWALEKEKAKLGRSEERDKEELEDLKFSLESQKQRNLQLNLLLEQQKQLLNESQQKIESQRMLYDAQLSEEQGRNLELQVLLESEKVRIREMSSTLDRERELHAQLQSSDGTGQSRPPLPSEDLLKELQKQLEEKHSRIVELLNETEKYKLDSLQTRQQMEKDRQVHRKTLQTEQEANTEGQKKMHELQSKVEDLQRQLEEKRQQVYKLDLEGQRLQGIMQEFQKQELEREEKRESRRILYQNLNEPTTWSLTSDRTRNWVLQQKIEGETKESNYAKLIEMNGGGTGCNHELEMIRQKLQCVASKLQVLPQKASERLQFETADDEDFIWVQENIDEIILQLQKLTGQQGEEPSLVSPSTSCGSLTERLLRQNAELTGHISQLTEEKNDLRNMVMKLEEQIRWYRQTGAGRDNSSRFSLNGGANIEAIIASEKEVWNREKLTLQKSLKRAEAEVYKLKAELRNDSLLQTLSPDSEHVTLKRIYGKYLRAESFRKALIYQKKYLLLLLGGFQECEDATLALLARMGGQPAFTDLEVITNRPKGFTRFRSAVRVSIAISRMKFLVRRWHRVTGSVSININRDGFGLNQGAEKTDSFYHSSGGLELYGEPRHTTYRSRSDLDYIRSPLPFQNRYPGTPADFNPGSLACSQLQNYDPDRALTDYITRLEALQRRLGTIQSGSTTQFHAGMRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Sheep
92
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99996 As Substrate

Site PTM Type Enzyme
K9 Methylation
K24 Acetylation
S31 Phosphorylation
K35 Acetylation
K37 Acetylation
S43 Phosphorylation
S45 Phosphorylation
Y66 Phosphorylation
S146 Phosphorylation
S153 Phosphorylation
S162 Phosphorylation
T208 Phosphorylation
S257 Phosphorylation
K267 Ubiquitination
Y308 Phosphorylation
K369 Ubiquitination
K374 Acetylation
S390 Phosphorylation
T400 Phosphorylation
K410 Ubiquitination
K449 Ubiquitination
S477 Phosphorylation
Y478 Phosphorylation
S479 Phosphorylation
T482 Phosphorylation
K572 Ubiquitination
K639 Ubiquitination
K654 Acetylation
K664 Acetylation
K685 Ubiquitination
S700 Phosphorylation
K713 Ubiquitination
K726 Ubiquitination
K738 Ubiquitination
K769 Ubiquitination
S778 Phosphorylation
K785 Ubiquitination
T795 Phosphorylation
K810 Ubiquitination
K845 Ubiquitination
S851 Phosphorylation
K913 Methylation
K952 Ubiquitination
K967 Ubiquitination
K981 Ubiquitination
S1041 Phosphorylation
K1066 Acetylation
K1094 Ubiquitination
K1097 Ubiquitination
K1147 Ubiquitination
K1163 Ubiquitination
K1173 Ubiquitination
K1182 Ubiquitination
Y1213 Phosphorylation
Y1214 Phosphorylation
S1230 Phosphorylation
Y1233 Phosphorylation
S1235 Phosphorylation
Y1245 Phosphorylation
Y1247 Phosphorylation
K1262 Ubiquitination
K1269 Ubiquitination
K1279 Ubiquitination
K1289 Acetylation
K1289 Ubiquitination
K1299 Acetylation
K1324 Ubiquitination
S1327 Phosphorylation
K1333 Ubiquitination
K1370 Ubiquitination
K1430 Ubiquitination
K1434 Ubiquitination
K1475 Ubiquitination
Y1487 Phosphorylation
K1492 Ubiquitination
K1515 Ubiquitination
K1519 Ubiquitination
K1526 Ubiquitination
S1564 Phosphorylation
S1641 Phosphorylation
S1642 Phosphorylation
K1664 Ubiquitination
S1667 Phosphorylation
K1673 Ubiquitination
K1726 Ubiquitination
S1765 Phosphorylation
S1768 Phosphorylation
T1791 Phosphorylation
S1794 Phosphorylation
S1797 Phosphorylation
S1866 Phosphorylation
K1872 Ubiquitination
S1882 Phosphorylation
S1907 Phosphorylation
S1977 Phosphorylation
K2089 Ubiquitination
S2104 Phosphorylation
T2108 Phosphorylation
K2148 Acetylation
K2303 Ubiquitination
K2320 Ubiquitination
K2342 Ubiquitination
S2376 Phosphorylation
K2475 Ubiquitination
S2476 Phosphorylation
T2481 Phosphorylation
Y2482 Phosphorylation
S2485 Phosphorylation
S2493 Phosphorylation
K2520 Ubiquitination
K2539 Ubiquitination
S2579 Phosphorylation
S2609 Phosphorylation
S2665 Phosphorylation
S2681 Phosphorylation
K2702 Ubiquitination
K2872 Ubiquitination
S2892 Phosphorylation
K2985 Ubiquitination
Y2987 Phosphorylation
Y3010 Phosphorylation
S3089 Phosphorylation
S3092 Phosphorylation
S3114 Phosphorylation
K3116 Ubiquitination
T3177 Phosphorylation
T3178 Phosphorylation
K3226 Ubiquitination
K3253 Ubiquitination
K3276 Ubiquitination
R3318 Methylation
S3328 Phosphorylation
K3445 Ubiquitination
Y3461 Phosphorylation
S3471 Phosphorylation
K3485 Ubiquitination
T3490 Phosphorylation
K3491 Ubiquitination
Y3495 Phosphorylation
K3518 Ubiquitination
K3525 Ubiquitination
K3532 Ubiquitination
S3573 Phosphorylation
S3576 Phosphorylation
K3606 Ubiquitination
K3615 Ubiquitination
S3637 Phosphorylation
K3659 Ubiquitination
K3664 Ubiquitination
S3684 Phosphorylation
S3690 Phosphorylation
K3699 Ubiquitination
T3736 Phosphorylation
S3812 Phosphorylation
Y3814 Phosphorylation
S3816 Phosphorylation
S3817 Phosphorylation
Y3823 Phosphorylation
S3833 Phosphorylation
S3835 Phosphorylation
Y3839 Phosphorylation
R3841 Methylation
S3842 Phosphorylation
T3853 Phosphorylation
S3865 Phosphorylation
Y3870 Phosphorylation
S3895 Phosphorylation
S3897 Phosphorylation
T3898 Phosphorylation

Research Backgrounds

Function:

Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus. Required for microtubule nucleation at the cis-side of the Golgi apparatus. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. In complex with PDE4DIP isoform 13/MMG8/SMYLE, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. In complex with PDE4DIP isoform 13/MMG8/SMYLE, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension also from the centrosome to the cell periphery.

Associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.

Subcellular Location:

Golgi apparatus. Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Cytoplasmic in parietal cells (PubMed:9915845). Recruited to the Golgi apparatus by GM130/GOLGA2 (PubMed:25657325). Localization at the centrosome versus Golgi apparatus may be cell line-dependent. In SKBr3 and HEK293F cells, exclusively located at the centrosome (PubMed:29162697). In HeLa, MDA-MB231 and RPE-1 cells, detected at the Golgi apparatus (PubMed:25217626, PubMed:29162697). In SK-BR-3 cells, recruited to the centrosome in the presence of CDK5RAP2 (PubMed:29162697).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Isoform 4: Highly expressed in skeletal muscle and in pancreas.

Subunit Structure:

Interacts with the regulatory region of protein kinase N (PKN), protein phosphatase 2A (PP2A), protein phosphatase 1 (PP1) and the immature non-phosphorylated form of PKC epsilon. Interacts with CIP4 and FNBP1. Interacts with chloride intracellular channel proteins CLIC1, CLIC4 and CLIC5. CSNK1D binding promotes its centrosomal subcellular location. Interacts with GM130/GOLGA2; leading to recruitment to the Golgi apparatus. Interacts with KCNQ1; targets protein kinase A (PKA) catalytic and regulatory subunits and protein phosphatase 1 (PP1), to the heterodimer KCNQ1-KCNE1. Interacts with PDE4DIP isoform 13/MMG8/SMYLE; this interaction stabilizes both proteins. In complex with PDE4DIP isoform 13, recruits CAMSAP2 to the Golgi apparatus. Forms a pericentrosomal complex with CDK5RAP2, EB1/MAPRE1 and PDE4DIP isoform 13; within this complex, MAPRE1 binding to CDK5RAP2 may be mediated by PDE4DIP. Interacts with MAPRE1 and MAPRE3. Interacts (via C-terminus) with CAMSAP2; this interaction is much stronger in the presence of PDE4DIP isoform 13/MMG8/SMYLE. Interacts with CAMSAP3. Interacts (via C-terminus) with the gamma-tubulin ring complex (gamma-TuRC), composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6.

Family&Domains:

RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

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