Ubinuclein Antibody - #DF8873

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Product: Ubinuclein Antibody
Catalog: DF8873
Description: Rabbit polyclonal antibody to Ubinuclein
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 121 kDa; 122kD(Calculated).
Uniprot: Q9NPG3
RRID: AB_2842069

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(88%), Sheep(88%), Rabbit(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
Ubinuclein Antibody detects endogenous levels of total Ubinuclein.
RRID:
AB_2842069
Cite Format: Affinity Biosciences Cat# DF8873, RRID:AB_2842069.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ubinuclein 1; Ubinuclein; Ubiquitously expressed nuclear protein; VT; VT4;

Immunogens

Immunogen:
Uniprot:

Q9NPG3(UBN1_HUMAN) >>Visit HPA database.

Gene(ID):
UBN1(29855)
Expression:
Q9NPG3 UBN1_HUMAN:

Ubiquitous. Also expressed in numerous tumors and cancer cell lines.

Sequence:
MSEPHRVQFTSLPGSLNPAFLKKSRKEEAGAGEQHQDCEPAAAAVRITLTLFEPDHKRCPEFFYPELVKNIRGKVKGLQPGDKKKDLSDPFNDEEKERHKVEALARKFEEKYGGKKRRKDRIQDLIDMGYGYDESDSFIDNSEAYDELVPASLTTKYGGFYINSGTLQFRQASESEDDFIKEKKKKSPKKRKLKEGGEKIKKKKKDDTYDKEKKSKKSKFSKAGFTALNASKEKKKKKYSGALSVKEMLKKFQKEKEAQKKREEEHKPVAVPSAEAQGLRELEGASDPLLSLFGSTSDNDLLQAATAMDSLTDLDLEHLLSESPEGSPFRDMDDGSDSLGVGLDQEFRQPSSLPEGLPAPLEKRVKELAQAARAAEGESRQKFFTQDINGILLDIEAQTRELSSQVRSGVYAYLASFLPCSKDALLKRARKLHLYEQGGRLKEPLQKLKEAIGRAMPEQMAKYQDECQAHTQAKVAKMLEEEKDKEQRDRICSDEEEDEEKGGRRIMGPRKKFQWNDEIRELLCQVVKIKLESQDLERNNKAQAWEDCVKGFLDAEVKPLWPKGWMQARTLFKESRRGHGHLTSILAKKKVMAPSKIKVKESSTKPDKKVSVPSGQIGGPIALPSDHQTGGLSIGASSRELPSQASGGLANPPPVNLEDSLDEDLIRNPASSVEAVSKELAALNSRAAGNSEFTLPAPSKAPAEKVGGVLCTEEKRNFAKPSPSAPPPASSLQSPLNFLAEQALALGQSSQEKKPESSGYKELSCQAPLNKGLPEVHQSKAKHHSLPRTSHGPQVAVPVPGPQVKVFHAGTQQQKNFTPPSPFANKLQGPKASPTQCHRSLLQLVKTAAKGQGFHPSAPATSGGLSASSSSSHKTPASSSSALSHPAKPHSVSSAGSSYKNNPFASSISKHGVSSGSSSSGGTPVQSSVSGSLVPGIQPPSVGQATSRPVPSSAGKKMPVSQKLTLVAPPGGPNGDSSGGTQGVAKLLTSPSLKPSAVSSVTSSTSLSKGASGTVLLAGSSLMASPYKSSSPKLSGAMSSNSLGIITPVPIPVHVLSFSADSSAKAGVSKDAIVTGPAPGSFHHGLGHSLLAGLHSSPPHAAPLPHAAVPTHIPQSLPGASQLHGKGPAVPRKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Bovine
88
Sheep
88
Dog
88
Horse
75
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPG3 As Substrate

Site PTM Type Enzyme
T10 Phosphorylation
S11 Phosphorylation
S15 Phosphorylation
Y64 Phosphorylation
K107 Acetylation
Y112 Phosphorylation
Y130 Phosphorylation
T166 Phosphorylation
S173 Phosphorylation
S175 Phosphorylation
S187 Phosphorylation
K222 Acetylation
S231 Phosphorylation
Y239 Phosphorylation
S240 Phosphorylation
S244 Phosphorylation
K267 Acetylation
S323 Phosphorylation
S327 Phosphorylation
S336 Phosphorylation
S338 Phosphorylation
S351 Phosphorylation
S352 Phosphorylation
K474 Ubiquitination
K477 Acetylation
S493 Phosphorylation
K530 Sumoylation
K541 Ubiquitination
K573 Methylation
S575 Phosphorylation
S584 Phosphorylation
S660 Phosphorylation
S671 Phosphorylation
S677 Phosphorylation
S764 Phosphorylation
T818 Phosphorylation
S821 Phosphorylation
K826 Acetylation
S833 Phosphorylation
S840 Phosphorylation
K888 Acetylation
S977 Phosphorylation
S978 Phosphorylation
T981 Phosphorylation
S990 Phosphorylation
S992 Phosphorylation
S996 Phosphorylation
S1021 Phosphorylation
S1025 Phosphorylation
S1030 Phosphorylation
S1031 Phosphorylation

Research Backgrounds

Function:

Acts as a novel regulator of senescence. Involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. Binds to proliferation-promoting genes. May be required for replication-independent chromatin assembly.

Subcellular Location:

Nucleus>Nucleoplasm. Nucleus>PML body. Cell junction>Tight junction.
Note: Localized as a nuclear speckled-like pattern in proliferating primary fibroblasts. Colocalizes with HIRA, PML and SP100 in PML bodies of senescent cells. Colocalizes with TJP1 and CLDN1. Detected along the upper granular cell layer of epidermis. When overexpressed, accumulates in the nucleus in cells showing defective cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Also expressed in numerous tumors and cancer cell lines.

Subunit Structure:

Component of a complex that includes at least ASF1A, CABIN1, HIRA, histone H3.3 and UBN1. Interacts with HIRA (via WD repeat domain); the interaction is direct. Interacts with ASF1A, CEBPA, TJP1, TJP2 and TJP3.

(Microbial infection) Interacts with Epstein-Barr virus BZLF1.

Family&Domains:

Belongs to the ubinuclein family.

References

1). ATP5O Hypo-crotonylation Caused by HDAC2 Hyper-Phosphorylation Is a Primary Detrimental Factor for Downregulated Phospholipid Metabolism under Chronic Stress. Research (Washington, D.C.), 2023 (PubMed: 38645677) [IF=11.0]

Application: WB    Species: Human    Sample:

Figure 4 Decreased ATP5O-K51 crotonylation conduced to reduced ATP5O stability and decreased lipid metabolism. (a–c) Transfection of ATP5O-WT or K51A (inactive mutant) plasmid (in pcDNA3.1) into 293 T cells and western blot by ATP5O-K51 cr antibody showed that ATP5O-K51A had significantly decreased crotonylation level (a, b), accordingly, ATP5O K51A-transfected 293 T cells had significantly lower cytoplasmic ATP level than ATP5O-WT-transfected 293 T cells. (d–g) Western blot showed that inhibition of ATP5O crotonylation with a competitive peptide TAC (CYGRKKRRQRRRATALYSAASKEKKL) significantly reduced ATP5O-K51 crotonylation (d, e) and gross ATP5O (f, g) levels, similar to CS treatment. (h, i). A region of Ubinuclein (UBN) shared the highest similarity with TAC peptide (Supp. Figure 8); however, TAC treatment did not change UBN level at all. (j, k) Western blot showed that inhibition of ATP5O crotonylation with TAC significantly decreased STAT5A protein level. (l) TAC treatment significantly reduced liver ATP level. (m–o) Quantitative lipidome showed that at the threshold of ATP5O−TAC/Ctr≥1.5 or ≤0.67 , there were 174 differentially upregulated or downregulated lipid metabolites (DULMs or DDLMs), among this, 22.41% (39/174) was phospholipid (PE & PC & PI), and 82.05% (32/39) of phospholipid was downregulated. (p) Representative downregulated phospholipid in TAC-treated group. (q–s) Blood biochemical index assay showed that multiple indexes in TAC group, including CREA, SOD, and HDL-C, were similar to CS group and significantly different from control group. Tubulin or actin was used as a loading control.

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