IBTK Antibody - #DF8881

(1)  
Product: IBTK Antibody
Catalog: DF8881
Description: Rabbit polyclonal antibody to IBTK
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 151kDa; 151kD(Calculated).
Uniprot: Q9P2D0
RRID: AB_2842077

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(90%), Bovine(82%), Horse(92%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
IBTK Antibody detects endogenous levels of total IBTK.
RRID:
AB_2842077
Cite Format: Affinity Biosciences Cat# DF8881, RRID:AB_2842077.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BTKI; IBtk; IBTK_HUMAN; inhibitor of Bruton agammaglobulinemia tyrosine kinase; Inhibitor of Bruton tyrosine kinase;

Immunogens

Immunogen:
Uniprot:

Q9P2D0(IBTK_HUMAN) >>Visit HPA database.

Gene(ID):
IBTK(25998)
Expression:
Q9P2D0 IBTK_HUMAN:

Expressed in DeFew, HEK293T, HeLa and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues.

Sequence:
MSSPMPDCTSKCRSLKHALDVLSVVTKGSENQIKAFLSSHCYNAATIKDVFGRNALHLVSSCGKKGVLDWLIQKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKNTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGIYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLVPRLVEGLNGHNCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQIQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGCLLDPNGEKCVTAPRQVSALHHKDIALSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGHMEYKVDPEHLKENGGQKICILAMDGAGRVFCWRSVNSSLKQCRWAYPRQVFISDIALNRNEILFVTQDGEGFRGRWFEEKRKSSEKKEILSNLHNSSSDVSYVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLREADEMDSIHDVTFQVGNRLFPAHKYILAVHSDFFQKLFLSDGNTSEFTDIYQKDEDSAGCHLFVVEKVHPDMFEYLLQFIYTDTCDFLTHGFKPRIHLNKNPEEYQGTLNSHLNKVNFHEDDNQKSAFEVYKSNQAQTVSERQKSKPKSCKKGKNIREDDPVRMLQTVAKKFDFSNLSSRLDGVRFENEKINVIAKNTGNKLKLSQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASSCAALEMPIHSDILKVILDYLYTDEAVVIKESQNVDFICSVLVVADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAKTKAKKKPRKRSDSSGGYNLSDIIQSPSSTGLLKSGKTNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTTGFHSDKIEAKVKPYVNGTSPVYSREDLKPWEKSPILKISAPQPIPSNRIDTTSSASWVAGSFSPVSPPVVDLRTIMEIEESRQKCGATPKSHLGKTVSHGVKLSQKQRKMIALTTKENNSGMNSMETVLFTPSKAPKPVNAWASSLHSVSSKSFRDFLLEEKKSVTSHSSGDHVKKVSFKGIENSQAPKIVRCSTHGTPGPEGNHISDLPLLDSPNPWLSSSVTAPSMVAPVTFASIVEEELQQEAALIRSREKPLALIQIEEHAIQDLLVFYEAFGNPEEFVIVERTPQGPLAVPMWNKHGC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Horse
92
Rabbit
91
Pig
90
Bovine
82
Sheep
64
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P2D0 As Substrate

Site PTM Type Enzyme
K11 Ubiquitination
K27 Ubiquitination
K64 Ubiquitination
K81 Ubiquitination
Y96 Phosphorylation
Y113 Phosphorylation
K128 Ubiquitination
K137 Ubiquitination
T285 Phosphorylation
T296 Phosphorylation
T300 Phosphorylation
K375 Ubiquitination
K381 Ubiquitination
K475 Ubiquitination
K505 Ubiquitination
T599 Phosphorylation
K608 Ubiquitination
Y630 Phosphorylation
K655 Ubiquitination
K670 Ubiquitination
K680 Ubiquitination
K687 Methylation
K687 Ubiquitination
K725 Ubiquitination
K726 Ubiquitination
K745 Ubiquitination
K751 Ubiquitination
S949 Phosphorylation
Y950 Phosphorylation
K981 Acetylation
K984 Acetylation
S990 Phosphorylation
S992 Phosphorylation
S993 Phosphorylation
Y996 Phosphorylation
S999 Phosphorylation
S1004 Phosphorylation
S1006 Phosphorylation
T1008 Phosphorylation
T1016 Phosphorylation
S1018 Phosphorylation
S1021 Phosphorylation
S1030 Phosphorylation
S1033 Phosphorylation
S1039 Phosphorylation
S1045 Phosphorylation
T1049 Phosphorylation
S1054 Phosphorylation
Y1064 Phosphorylation
T1068 Phosphorylation
S1069 Phosphorylation
Y1072 Phosphorylation
S1073 Phosphorylation
K1078 Ubiquitination
K1082 Ubiquitination
S1083 Phosphorylation
K1087 Ubiquitination
S1096 Phosphorylation
S1113 Phosphorylation
S1116 Phosphorylation
T1138 Phosphorylation
K1140 Methylation
S1141 Phosphorylation
K1145 Methylation
K1145 Ubiquitination
K1152 Ubiquitination
T1181 Phosphorylation
S1198 Phosphorylation
S1200 Phosphorylation P05771 (PRKCB)
K1202 Ubiquitination
S1203 Phosphorylation P05771 (PRKCB)
K1212 Ubiquitination
K1230 Methylation
K1239 Ubiquitination
K1350 Ubiquitination

Research Backgrounds

Function:

Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein.
Note: Translocates to the plasma membrane upon IgM stimulation.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in DeFew, HEK293T, HeLa and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues.

Subunit Structure:

Interacts with the PH domain of BTK. Isoform 2 does not interact with BTK.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.