AKAP11 Antibody - #DF8884

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Product: AKAP11 Antibody
Catalog: DF8884
Description: Rabbit polyclonal antibody to AKAP11
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Rabbit
Mol.Wt.: 210 kDa; 211kD(Calculated).
Uniprot: Q9UKA4
RRID: AB_2842080

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
AKAP11 Antibody detects endogenous levels of total AKAP11.
RRID:
AB_2842080
Cite Format: Affinity Biosciences Cat# DF8884, RRID:AB_2842080.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A-kinase anchor protein 11; A-kinase anchor protein 220 kDa; AKA11_HUMAN; AKAP 220; AKAP-11; AKAP11; hAKAP220; PRKA11; Protein kinase A-anchoring protein 11;

Immunogens

Immunogen:
Uniprot:

Q9UKA4(AKA11_HUMAN) >>Visit HPA database.

Gene(ID):
AKAP11(11215)
Expression:
Q9UKA4 AKA11_HUMAN:

Expressed in heart, brain, lung, liver, kidney, testis and ovary. Weakly expressed in skeletal muscle, pancreas and spleen.

Sequence:
MATFRNNHMKTKASVRKSFSEDVFQSVKSLLQSQKELCSVTAEDCLQQDEHANLTEVTFLGFNEETDAAHIQDLAAVSLELPDILNSLHFCSLNENEIICMKNINKPLDISSDPLNQSHPSGMLCVMRVSPTSPRLRIDFIFSLLSKYATGIRYTLDTFLHQKHQLETTDEDDDDTNQSVSSIEDDFVTAFEHLEEEETSKPYNDGMNITVLRSQCDAASQTVTGHHLETHDLKILISSGQQKSLAKPSTSSVNVLGHKELPSVKTSVTTSISEPWTQRSFYRSSNASDKDSDLQKTFFSSSPAYSSESECSSPSPVIFLDEEGYQKSLKAKLELPKIPVMKDDIEDSDSEVSEFFDSFDQFDELEQTLETCLFNKDPVIGKSSQRKGHKHGKSCMNPQKFKFDRPALPANVRKPTPRKPESPYGNLCDAPDSPRPVKASREDSGLFSPIRSSAFSPLGGCTPAECFCQTDIGGDRIHENHDSVYYTYEDYAKSISCEVLGSVLRTHHTNTLSNINSIKHGENKTVTFKHGNLDQKNKSKNKSLMIKDSIQKFAADLVEKSFGSAFKDLQKGVSSCTNALYHLAIKLTSSVLQMAFDELRRQRAFSLKERAISGLANFLVSEALSNALKDLQYVKKQIFTNTVARFAADLAEELVFEGIMEVCQFSYPQTPASPQCGSFDFEDKVVKLYAKDLSESVIQEAFIELSQVDVTFTTKAAVSVSTDNIKYVSAESVVPSTQAVTFSPSFHNQAIMVTKPVQEYKKEYTVQQALFCTSGIVTSIPVPLAGSALLPYHISSTACQAKAHLSSDDSNSNGDSAQVHIATKNREEKAACLRNICLPSEHNPGNQNDFKPTNDDIEMQSSSKLPNDPAIISNFSAAVVHTIVNETLESMTSLEVTKMVDERTDYLTKSLKEKTPPFSHCDQAVLQCSEASSNKDMFADRLSKSIIKHSIDKSKSVIPNIDKNAVYKESLPVSGEESQLTPEKSPKFPDSQNQLTHCSLSAAKDCVPECKVSMVHGSSLETLPSCPAVTGQKSDLKESAKDQPLKKHNLNSTSLEALSFGQENPFPHSHTFSSTALTCVDGLHVEDKQKVRDRNVIPDTPPSTPLVPSRASSEWDIKKLTKKLKGELAKEFAPATPPSTPHNSSVGSLSENEQNTIEKEEFMLKLMRSLSEEVESSESGELPEVDVKSEHSGKKVQFAEALATHILSLATEMAASHLDNKIIQEPKVKNPCLNVQSQRSVSPTFLNPSDENLKTLCNFAGDLAAEVITEAEKIAKVRNCMLFKQKKNSCYADGDEDYKVEEKLDIEAVVHPREVDPFILSLPPSSCMSGLMYKYPSCESVTDEYAGHLIQILKQEGGNSELIMDQYANRLAYRSVKSGLQEAAKTTKVQCNSRMFPVPSSQVKTNKELLMFSNKEHHQEADKKRQSKRNEGYFCKNQTCERTLDPYRNEVSQLYSFSTSLVHSITKDAKEELTASLVGLPKSLTDSCLFEKSGYEEDNECHVTPELPKSLQPSSQNHRFYHSTGSLNGYGCGDNVVQAVEQYAKKVVDDTLELTLGSTVFRVSETTKSADRVTYAEKLSPLTGQACRYCDLKELHNCTGNSSQHFFRQGSLASSKPASNPKFSSRYQKSRIFHLSVPQIHVNLDKKAVLAEKIVAEAIEKAERELSSTSLAADSGIGQEGASFAESLATETMTAAVTNVGHAVSSSKEIEDFQSTESVSSQQMNLSIGDDSTGSWSNLSFEDEHQDESSSFHHLSESNGNSSSWSSLGLEGDLYEDNLSFPTSDSDGPDDKDEEHEDEVEGLGQDGKTLLITNIDMEPCTVDPQLRIILQWLIASEAEVAELYFHDSANKEFMLLSKQLQEKGWKVGDLLQAVLQYYEVMEKASSEERCKSLFDWLLENA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Xenopus
75
Chicken
75
Horse
0
Bovine
0
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKA4 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
S18 Phosphorylation
S20 Phosphorylation
S29 Phosphorylation
S130 Phosphorylation
S133 Phosphorylation
T150 Phosphorylation
T199 Phosphorylation
S200 Phosphorylation
S239 Phosphorylation
K243 Ubiquitination
T250 Phosphorylation
S273 Phosphorylation
S285 Phosphorylation
S288 Phosphorylation
S328 Phosphorylation
S422 Phosphorylation
Y424 Phosphorylation
S433 Phosphorylation
K438 Methylation
S440 Phosphorylation
S444 Phosphorylation
S448 Phosphorylation
S456 Phosphorylation
Y485 Phosphorylation
Y486 Phosphorylation
Y488 Phosphorylation
Y491 Phosphorylation
S502 Phosphorylation
K519 Ubiquitination
K529 Methylation
K560 Ubiquitination
S606 Phosphorylation
K608 Ubiquitination
S613 Phosphorylation
S621 Phosphorylation
S673 Phosphorylation
S812 Phosphorylation
K909 Ubiquitination
S919 Phosphorylation
S945 Phosphorylation
K963 Ubiquitination
T981 Phosphorylation
S985 Phosphorylation
S1013 Phosphorylation
S1018 Phosphorylation
S1019 Phosphorylation
T1100 Phosphorylation
S1103 Phosphorylation
T1104 Phosphorylation
S1109 Phosphorylation
S1113 Phosphorylation
T1136 Phosphorylation P49841 (GSK3B)
T1140 Phosphorylation P49841 (GSK3B)
S1169 Phosphorylation
S1171 Phosphorylation
S1176 Phosphorylation
T1204 Phosphorylation
S1240 Phosphorylation
S1242 Phosphorylation
T1244 Phosphorylation
K1287 Acetylation
S1289 Phosphorylation
Y1291 Phosphorylation
Y1298 Phosphorylation
K1299 Acetylation
S1337 Phosphorylation
S1340 Phosphorylation
T1342 Phosphorylation
S1360 Phosphorylation
Y1367 Phosphorylation
K1377 Ubiquitination
K1388 Ubiquitination
Y1455 Phosphorylation
K1470 Ubiquitination
S1476 Phosphorylation
T1485 Phosphorylation
S1487 Phosphorylation
K1492 Ubiquitination
S1493 Phosphorylation
T1504 Phosphorylation
S1523 Phosphorylation
T1524 Phosphorylation
S1526 Phosphorylation
Y1530 Phosphorylation
S1569 Phosphorylation
K1578 Ubiquitination
S1580 Phosphorylation
T1583 Phosphorylation
S1611 Phosphorylation
K1616 Ubiquitination
S1619 Phosphorylation
S1636 Phosphorylation
S1737 Phosphorylation

Research Backgrounds

Function:

Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Cytoplasmic in premeiotic pachytene spermatocytes and in the centrosome of developing postmeiotic germ cells, while a midpiece/centrosome localization was found in elongating spermatocytes and mature sperm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, brain, lung, liver, kidney, testis and ovary. Weakly expressed in skeletal muscle, pancreas and spleen.

Family&Domains:

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Belongs to the AKAP110 family.

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