MKL2 Antibody - #DF8885
(1)
| Product: | MKL2 Antibody |
| Catalog: | DF8885 |
| Description: | Rabbit polyclonal antibody to MKL2 |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Mol.Wt.: | 118kDa; 118kD(Calculated). |
| Uniprot: | Q9ULH7 |
| RRID: | AB_2842081 |
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
MKL2 Antibody detects endogenous levels of total MKL2.
RRID:
AB_2842081
Cite Format: Affinity Biosciences Cat# DF8885, RRID:AB_2842081.
Cite Format: Affinity Biosciences Cat# DF8885, RRID:AB_2842081.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
FLJ31823; Megakaryoblastic leukemia 2; megakaryoblastic leukemia 2 protein; MKL/myocardin-like 2; MKL/myocardin-like protein 2; Mkl2; MKL2_HUMAN; MRTF-B; Myocardin-related transcription factor B; NPD001;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q9ULH7 MRTFB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIDSSKKQQQGFPEILTAGDFEPLKEKECLEGSNQKSLKEVLQLRLQQRRTREQLVDQGIMPPLKSPAAFHEQIKSLERARTENFLKHKIRSRPDRSELVRMHILEETFAEPSLQATQMKLKRARLADDLNEKIAQRPGPMELVEKNILPVDSSVKEAIIGVGKEDYPHTQGDFSFDEDSSDALSPDQPASQESQGSAASPSEPKVSESPSPVTTNTPAQFASVSPTVPEFLKTPPTADQPPPRPAAPVLPTNTVSSAKPGPALVKQSHPKNPNDKHRSKKCKDPKPRVKKLKYHQYIPPDQKGEKNEPQMDSNYARLLQQQQLFLQLQILSQQKQHYNYQTILPAPFKPLNDKNSNSGNSALNNATPNTPRQNTSTPVRKPGPLPSSLDDLKVSELKTELKLRGLPVSGTKPDLIERLKPYQEVNSSGLAAGGIVAVSSSAIVTSNPEVTVALPVTTLHNTVTSSVSTLKAELPPTGTSNATRVENVHSPLPISPSPSEQSSLSTDDTNMADTFTEIMTMMSPSQFLSSSPLRMTNNEDSLSPTSSTLSNLELDAAEKDRKLQEKEKQIEELKRKLEQEQKLVEVLKMQLEVEKRGQQQRPLEAQPSAPGHSVKSDQKHGSLGSSIKDEASLPDCSSSRQPIPVASHAVGQPVSTGGQTLVAKKAVVIKQEVPVGQAEQQSVVSQFYVSSQGQPPPAVVAQPQALLTTQTAQLLLPVSIQGSSVTSVQLPVGSLKLQTSPQAGMQTQPQIATAAQIPTAALASGLAPTVPQTQDTFPQHVLSQPQQVRKVFTNSASSNTVLPYQRHPAPAVQQPFINKASNSVLQSRNAPLPSLQNGPNTPNKPSSPPPPQQFVVQHSLFGSPVAKTKDPPRYEEAIKQTRSTQAPLPEISNAHSQQMDDLFDILIKSGEISLPIKEEPSPISKMRPVTASITTMPVNTVVSRPPPQVQMAPPVSLEPMGSLSASLENQLEAFLDGTLPSANEIPPLQSSSEDREPFSLIEDLQNDLLSHSGMLDHSHSPMETSETQFAAGTPCLSLDLSDSNLDNMEWLDITMPNSSSGLTPLSTTAPSMFSADFLDPQDLPLPWD
PTMs - Q9ULH7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S66 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| T82 | Phosphorylation | Uniprot | |
| K87 | Methylation | Uniprot | |
| K146 | Acetylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| T234 | Phosphorylation | Uniprot | |
| T237 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| K293 | Methylation | Uniprot | |
| K349 | Acetylation | Uniprot | |
| T367 | Phosphorylation | Uniprot | |
| T370 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| K381 | Methylation | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| S541 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S550 | Phosphorylation | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| S622 | Phosphorylation | Uniprot | |
| K628 | Sumoylation | Uniprot | |
| S632 | Phosphorylation | Uniprot | |
| R806 | Methylation | Uniprot | |
| S823 | Phosphorylation | Uniprot | |
| S834 | Phosphorylation | Uniprot | |
| T841 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| S847 | Phosphorylation | Uniprot | |
| S863 | Phosphorylation | Uniprot | |
| Y874 | Phosphorylation | Uniprot | |
| S909 | Phosphorylation | Uniprot | |
| S913 | Phosphorylation | Uniprot | |
| K917 | Ubiquitination | Uniprot | |
| S921 | Phosphorylation | Uniprot | |
| S924 | Phosphorylation | Uniprot | |
| S1041 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.
PTMs:
O-glycosylated.
Subcellular Location:
Nucleus.
Subunit Structure:
Interacts with MRTFA and SRF.
Family&Domains:
The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.
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