TRIM33 Antibody - #DF8887

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Product: TRIM33 Antibody
Catalog: DF8887
Description: Rabbit polyclonal antibody to TRIM33
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 123 kDa; 123kD(Calculated).
Uniprot: Q9UPN9
RRID: AB_2842083

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TRIM33 Antibody detects endogenous levels of total TRIM33.
RRID:
AB_2842083
Cite Format: Affinity Biosciences Cat# DF8887, RRID:AB_2842083.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

8030451N04Rik; AI413936; cb1085; DKFZp586K1123; E3 ubiquitin-protein ligase TRIM33; EC 6.3.2.-; Ectodermin; Ectodermin homolog; FLJ11429; FLJ32925; id:ibd2175; MGC136680; mKIAA1113; OTTHUMP00000013662; OTTHUMP00000013663; Protein Rfg7; PTC7; Ret fused gene 7; RET-fused gene 7 protein; RFG7; Rfg7 protein; TF1G; TIF1-gamma; TIF1G; TIF1GAMMA; TIFGAMMA; Transcription intermediary factor 1-gamma; Transcriptional intermediary factor 1 gamma; TRI33_HUMAN; trim33; Tripartite motif containing 33; Tripartite motif containing 33 protein; tripartite motif-containing 33; Tripartite motif-containing protein 33; wu:fc17f10; zgc:136680;

Immunogens

Immunogen:
Uniprot:

Q9UPN9(TRI33_HUMAN) >>Visit HPA database.

Gene(ID):
TRIM33(51592)
Expression:
Q9UPN9 TRI33_HUMAN:

Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.

Sequence:
MAENKGGGEAESGGGGSGSAPVTAGAAGPAAQEAEPPLTAVLVEEEEEEGGRAGAEGGAAGPDDGGVAAASSGSAQAASSPAASVGTGVAGGAVSTPAPAPASAPAPGPSAGPPPGPPASLLDTCAVCQQSLQSRREAEPKLLPCLHSFCLRCLPEPERQLSVPIPGGSNGDIQQVGVIRCPVCRQECRQIDLVDNYFVKDTSEAPSSSDEKSEQVCTSCEDNASAVGFCVECGEWLCKTCIEAHQRVKFTKDHLIRKKEDVSESVGASGQRPVFCPVHKQEQLKLFCETCDRLTCRDCQLLEHKEHRYQFLEEAFQNQKGAIENLLAKLLEKKNYVHFAATQVQNRIKEVNETNKRVEQEIKVAIFTLINEINKKGKSLLQQLENVTKERQMKLLQQQNDITGLSRQVKHVMNFTNWAIASGSSTALLYSKRLITFQLRHILKARCDPVPAANGAIRFHCDPTFWAKNVVNLGNLVIESKPAPGYTPNVVVGQVPPGTNHISKTPGQINLAQLRLQHMQQQVYAQKHQQLQQMRMQQPPAPVPTTTTTTQQHPRQAAPQMLQQQPPRLISVQTMQRGNMNCGAFQAHQMRLAQNAARIPGIPRHSGPQYSMMQPHLQRQHSNPGHAGPFPVVSVHNTTINPTSPTTATMANANRGPTSPSVTAIELIPSVTNPENLPSLPDIPPIQLEDAGSSSLDNLLSRYISGSHLPPQPTSTMNPSPGPSALSPGSSGLSNSHTPVRPPSTSSTGSRGSCGSSGRTAEKTSLSFKSDQVKVKQEPGTEDEICSFSGGVKQEKTEDGRRSACMLSSPESSLTPPLSTNLHLESELDALASLENHVKIEPADMNESCKQSGLSSLVNGKSPIRSLMHRSARIGGDGNNKDDDPNEDWCAVCQNGGDLLCCEKCPKVFHLTCHVPTLLSFPSGDWICTFCRDIGKPEVEYDCDNLQHSKKGKTAQGLSPVDQRKCERLLLYLYCHELSIEFQEPVPASIPNYYKIIKKPMDLSTVKKKLQKKHSQHYQIPDDFVADVRLIFKNCERFNEMMKVVQVYADTQEINLKADSEVAQAGKAVALYFEDKLTEIYSDRTFAPLPEFEQEEDDGEVTEDSDEDFIQPRRKRLKSDERPVHIK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
89
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UPN9 As Substrate

Site PTM Type Enzyme
S169 Phosphorylation
Y197 Phosphorylation
K252 Acetylation
K305 Ubiquitination
K320 Ubiquitination
K329 Ubiquitination
K334 Sumoylation
K334 Ubiquitination
K356 Ubiquitination
K378 Ubiquitination
K389 Ubiquitination
K394 Ubiquitination
R440 Methylation
R458 Methylation
K481 Sumoylation
K504 Ubiquitination
T505 Phosphorylation
R515 Methylation
Y524 Phosphorylation
R535 Methylation
R555 Methylation
R577 Methylation
C582 S-Nitrosylation
R591 Methylation
R598 Methylation
R604 Methylation
Y610 Phosphorylation
S622 Phosphorylation
T643 Phosphorylation
S644 Phosphorylation
S661 Phosphorylation
S715 Phosphorylation
S727 Phosphorylation
S744 Phosphorylation
S747 Phosphorylation
K763 Acetylation
K763 Sumoylation
K763 Ubiquitination
S767 Phosphorylation
K769 Acetylation
K769 Sumoylation
K769 Ubiquitination
K776 Acetylation
K776 Sumoylation
S787 Phosphorylation
S789 Phosphorylation
K793 Acetylation
K793 Sumoylation
K793 Ubiquitination
S803 Phosphorylation
S808 Phosphorylation
S809 Phosphorylation
S812 Phosphorylation
S813 Phosphorylation
T815 Phosphorylation
S819 Phosphorylation
T820 Phosphorylation
S852 Phosphorylation
S855 Phosphorylation
S856 Phosphorylation
K861 Acetylation
K861 Sumoylation
S862 Phosphorylation
S949 Phosphorylation
K950 Acetylation
K951 Acetylation
K953 Acetylation
K953 Sumoylation
K953 Ubiquitination
S959 Phosphorylation
K998 Ubiquitination
K999 Ubiquitination
K1007 Sumoylation
K1007 Ubiquitination
K1033 Ubiquitination
Y1048 Phosphorylation
T1051 Phosphorylation
K1057 Sumoylation
K1057 Ubiquitination
K1067 Ubiquitination
T1102 Phosphorylation
S1105 Phosphorylation
K1118 Sumoylation
S1119 Phosphorylation
K1127 Acetylation

Research Backgrounds

Function:

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).

PTMs:

Sumoylated with SUMO1.

Subcellular Location:

Nucleus.
Note: In discrete nuclear dots resembling nuclear bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.

Subunit Structure:

Homooligomer and heterooligomer with TRIM24 and TRIM28 family members. Interacts with SMAD4 in unstimulated cells. Found in a complex with SMAD2 and SMAD3 upon addition of TGF-beta. Interacts with SMAD2 and SMAD3. Interacts with SMAD4 under basal and induced conditions and, upon TGF-beta signaling, with activated SMAD2. Forms a ternary complex with SMAD4 and SMAD2 upon TGF-beta signaling.

Family&Domains:

Belongs to the TRIM/RBCC family.

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