TRIM35 Antibody | Affinity Biosciences

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TRIM35 Antibody - Western blot analysis of TRIM35 using HuvEc whole cell lysates.

Product:	TRIM35 Antibody
Catalog:	DF8888
Description:	Rabbit polyclonal antibody to TRIM35
Application:	WB
Reactivity:	Human
Prediction:	Rabbit, Dog
Mol.Wt.:	57 kDa; 57kD(Calculated).
Uniprot:	Q9UPQ4
RRID:	AB_2842084

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

TRIM35 Antibody detects endogenous levels of total TRIM35.

RRID:

AB_2842084
Cite Format: Affinity Biosciences Cat# DF8888, RRID:AB_2842084.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

0710005M05Rik; A430106H13Rik; AW046487; Hemopoietic lineage switch protein 5; HLS5; KIAA1098; Macrophage derived apoptosis inducing RBCC protein; MAIR; MGC17233; mKIAA1098; NC8; Protein MAIR; Protein Nc8; TRI35_HUMAN; TRIM35; Tripartite motif containing 35; Tripartite motif containing protein 35; Tripartite motif-containing protein 35;

Immunogens

Immunogen:

Uniprot:

Q9UPQ4(TRI35_HUMAN) >>Visit HPA database.

Gene(ID):

TRIM35(23087)

Sequence:

Q9UPQ4 TRI35_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRAKCRNMEHALREKAKAFWAMRRSYEAIAKHNQVEAAWLEGRIRQEFDKLREFLRVEEQAILDAMAEETRQKQLLADEKMKQLTEETEVLAHEIERLQMEMKEDDVSFLMKHKSRKRRLFCTMEPEPVQPGMLIDVCKYLGSLQYRVWKKMLASVESVPFSFDPNTAAGWLSVSDDLTSVTNHGYRVQVENPERFSSAPCLLGSRVFSQGSHAWEVALGGLQSWRVGVVRVRQDSGAEGHSHSCYHDTRSGFWYVCRTQGVEGDHCVTSDPATSPLVLAIPRRLRVELECEEGELSFYDAERHCHLYTFHARFGEVRPYFYLGGARGAGPPEPLRICPLHISVKEELDG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Dog

Pig

Bovine

Horse

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UPQ4 As Substrate

Q9UPQ4

Site	PTM Type	Source
M1	Acetylation	Uniprot
S4	Phosphorylation	Uniprot
S8	Phosphorylation	Uniprot
K61	Ubiquitination	Uniprot
S65	Phosphorylation	Uniprot
K138	Methylation	Uniprot
K138	Ubiquitination	Uniprot
K160	Ubiquitination	Uniprot
K174	Ubiquitination	Uniprot
K193	Ubiquitination	Uniprot
K216	Ubiquitination	Uniprot
K223	Ubiquitination	Uniprot
K225	Ubiquitination	Uniprot
K246	Ubiquitination	Uniprot
K255	Ubiquitination	Uniprot
K257	Acetylation	Uniprot
K260	Acetylation	Uniprot
S418	Phosphorylation	Uniprot
K488	Ubiquitination	Uniprot

Research Backgrounds

Q9UPQ4_TRI35_HUMAN

Function:

Reduces FGFR1-dependent tyrosine phosphorylation of PKM, inhibiting PKM-dependent lactate production, glucose metabolism, and cell growth. Involved in the cell death mechanism (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.
Note: Found predominantly in cytoplasm with a granular distribution. Found in punctuate nuclear bodies (By similarity).

Subunit Structure:

Interacts with PKM isoform M2, but not isoform M1; this interaction may compete with that between PKM and FGFR1, and hence reduces FGFR1-dependent tyrosine phosphorylation of PKM.

Family&Domains:

The RING finger domain and the coiled-coil region are required for the apoptosis-inducing activity.

Belongs to the TRIM/RBCC family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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TRIM35 Antibody - #DF8888