WNK2 Antibody - #DF8894
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8894, RRID:AB_2842090.
Fold/Unfold
Antigen NY-CO-43; KIAA1760; mitogen-activated protein kinase kinase kinase; NY CO 43; P/OKcl.13; PRKWNK2; Protein kinase lysine deficient 2; Protein kinase with no lysine 2; SDCCAG43; Serine/Threonine protein kinase WNK2; Serologically defined colon cancer antigen 43; WNK lysine deficient protein kinase 2;
Immunogens
Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart, brain, skeletal muscle and colon.
- Q9Y3S1 WNK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQGPCRGLTLPCLPWRRAACGAVFLSLFSAESAQSKQPPDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y3S1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T13 | Phosphorylation | Uniprot | |
| R19 | Methylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| Y210 | Phosphorylation | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| T347 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| Y442 | Phosphorylation | Uniprot | |
| T473 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| S1150 | Phosphorylation | Uniprot | |
| S1152 | Phosphorylation | Uniprot | |
| T1175 | Phosphorylation | Uniprot | |
| S1262 | Phosphorylation | Uniprot | |
| S1271 | Phosphorylation | Uniprot | |
| S1588 | Phosphorylation | Uniprot | |
| T1611 | Phosphorylation | Uniprot | |
| R1631 | Methylation | Uniprot | |
| S1685 | Phosphorylation | Uniprot | |
| S1689 | Phosphorylation | Uniprot | |
| Y1693 | Phosphorylation | Uniprot | |
| S1736 | Phosphorylation | Uniprot | |
| S1777 | Phosphorylation | Uniprot | |
| S1798 | Phosphorylation | Uniprot | |
| S1801 | Phosphorylation | Uniprot | |
| S1805 | Phosphorylation | Uniprot | |
| S1817 | Phosphorylation | Uniprot | |
| S1818 | Phosphorylation | Uniprot | |
| S1843 | Phosphorylation | Uniprot | |
| S1844 | Phosphorylation | Uniprot | |
| S1846 | Phosphorylation | Uniprot | |
| S1862 | Phosphorylation | Uniprot | |
| S1889 | Phosphorylation | Uniprot | |
| T1894 | Phosphorylation | Uniprot | |
| S1936 | Phosphorylation | Uniprot | |
| S2056 | Phosphorylation | Uniprot | |
| S2067 | Phosphorylation | Uniprot | |
| T2078 | Phosphorylation | Uniprot | |
| S2091 | Phosphorylation | Uniprot | |
| S2101 | Phosphorylation | Uniprot | |
| S2110 | Phosphorylation | Uniprot | |
| S2128 | Phosphorylation | Uniprot | |
| S2129 | Phosphorylation | Uniprot | |
| K2155 | Ubiquitination | Uniprot | |
| T2157 | Phosphorylation | Uniprot | |
| T2159 | Phosphorylation | Uniprot | |
| T2173 | Phosphorylation | Uniprot |
PTMs - Q9Y3S1 As Enzyme
Research Backgrounds
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.
Autophosphorylated.
Cytoplasm. Cell membrane.
Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart, brain, skeletal muscle and colon.
Forms a complex with the phosphorylated form of STK39.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
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