MAFB Antibody - #DF8895

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Product: MAFB Antibody
Catalog: DF8895
Description: Rabbit polyclonal antibody to MAFB
Application: WB
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 36 kDa; 36kD(Calculated).
Uniprot: Q9Y5Q3
RRID: AB_2842091

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 100ul $280 In stock
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Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Rabbit(92%), Dog(100%), Chicken(83%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
MAFB Antibody detects endogenous levels of total MAFB.
RRID:
AB_2842091
Cite Format: Affinity Biosciences Cat# DF8895, RRID:AB_2842091.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Kreisler; Kreisler (mouse) maf related leucine zipper homolog; Kreisler maf related leucine zipper homolog; KRML; Maf-B; Mafb; MAFB/Kreisler basic region/leucine zipper transcription factor; MAFB_HUMAN; MGC43127; Segmentation protein KR; Transcription factor MafB; V maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian); V-maf musculoaponeurotic fibrosarcoma oncogene homolog B;

Immunogens

Immunogen:

A synthesized peptide derived from human MAFB, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9Y5Q3(MAFB_HUMAN) >>Visit HPA database.

Gene(ID):
MAFB(9935)
Expression:
Q9Y5Q3 MAFB_HUMAN:

Ubiquitous.

Sequence:
MAAELSMGPELPTSPLAMEYVNDFDLLKFDVKKEPLGRAERPGRPCTRLQPAGSVSSTPLSTPCSSVPSSPSFSPTEQKTHLEDLYWMASNYQQMNPEALNLTPEDAVEALIGSHPVPQPLQSFDSFRGAHHHHHHHHPHPHHAYPGAGVAHDELGPHAHPHHHHHHQASPPPSSAASPAQQLPTSHPGPGPHATASATAAGGNGSVEDRFSDDQLVSMSVRELNRHLRGFTKDEVIRLKQKRRTLKNRGYAQSCRYKRVQQKHHLENEKTQLIQQVEQLKQEVSRLARERDAYKVKCEKLANSGFREAGSTSDSPSSPEFFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
92
Xenopus
83
Zebrafish
83
Chicken
83
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context.

PTMs:

Phosphorylated by GSK3 and MAPK13 on serine and threonine residues.

Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Family&Domains:

The leucine-zipper domain is involved in the interaction with LRPICD.

Belongs to the bZIP family. Maf subfamily.

References

1). Inhibition of spinal BRD4 alleviates pyroptosis and M1 microglia polarization via STING-IRF3 pathway in morphine-tolerant rats. European journal of pharmacology, 2024 (PubMed: 38432572) [IF=4.2]

Application: WB    Species: Rat    Sample: spinal cord

Fig. 7. Role of spinal STING-IRF3 pathway in morphine tolerance. (a–f) Representative Western blot results and subsequent quantitative analysis of STING, p-TBK1, TBK1, p-IRF3, and IRF3 expression in spinal cord (****p < 0.0001 vs. NS group. n = 6 per group). (g) Representative photomicrographs of cell localization of STING (red) with neurons (NeuN), microglia (Iba1), and astrocytes (GFAP) in spinal dorsal horn of Naïve, NS and MT group rats (n = 3 per group).
2). A New Way for Beta Cell Neogenesis: Transdifferentiation from Alpha Cells Induced by Glucagon-Like Peptide 1. Journal of Diabetes Research, 2019 (PubMed: 31001561) [IF=3.6]

Application: WB    Species: rat    Sample: islets

Figure 5: | Western blotting analysis for Akt, FoxO1, Pdx1, MafA, and MafB in rat islets. GAPDH was used as a loading control (#P < 0 05 vs.NC; ∗P < 0 05 vs. DM; •P < 0 05 vs. GLP1 50 μg/kg; and ΔP < 0 05 vs. GLP1 100 μg/kg). GLP1, glucagon-like peptide 1; NC, negative control;DM, diabetes mellitus; Akt, AKT kinase; FoxO1, forkhead box O 1; Pdx1, pancreatic and duodenal homeobox 1; MafA, MAF BZIP transcription factor A; MafB, MAF BZIP transcription factor B; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
3). The immunomodulatory role of the MAFB gene in hepatocellular carcinoma and its impact on biological activities. Gene, 2025 (PubMed: 39447710) [IF=3.5]

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