HJURP Antibody - #DF8942

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Product: HJURP Antibody
Catalog: DF8942
Description: Rabbit polyclonal antibody to HJURP
Application: WB
Reactivity: Human
Prediction: Pig, Horse, Dog
Mol.Wt.: 84 kDa; 84kD(Calculated).
Uniprot: Q8NCD3
RRID: AB_2842138

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(80%), Horse(100%), Dog(83%)
Clonality:
Polyclonal
Specificity:
HJURP Antibody detects endogenous levels of total HJURP.
RRID:
AB_2842138
Cite Format: Affinity Biosciences Cat# DF8942, RRID:AB_2842138.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

14-3-3-associated AKT substrate; FAKTS; fetal liver expressing gene 1; Fetal liver-expressing gene 1 protein; FLEG1; hFLEG1; HJURP; Holliday junction recognition protein; Up-regulated in lung cancer 9; URLC9;

Immunogens

Immunogen:
Uniprot:

Q8NCD3(HJURP_HUMAN) >>Visit HPA database.

Gene(ID):
HJURP(55355)
Expression:
Q8NCD3 HJURP_HUMAN:

According to PubMed:17256767, highly expressed in the thymus with lower levels in the placenta, small intestine, liver, skeletal muscle, and colon. According to PubMed:17823411, highly expressed in testis, and at a relatively lower level in thymus and bone marrow. Significantly overexpressed in many lung cancer samples, compared with normal lung.

Sequence:
MLGTLRAMEGEDVEDDQLLQKLRASRRRFQRRMQRLIEKYNQPFEDTPVVQMATLTYETPQGLRIWGGRLIKERNEGEIQDSSMKPADRTDGSVQAAAWGPELPSHRTVLGADSKSGEVDATSDQEESVAWALAPAVPQSPLKNELRRKYLTQVDILLQGAEYFECAGNRAGRDVRVTPLPSLASPAVPAPGYCSRISRKSPGDPAKPASSPREWDPLHPSSTDMALVPRNDSLSLQETSSSSFLSSQPFEDDDICNVTISDLYAGMLHSMSRLLSTKPSSIISTKTFIMQNWNSRRRHRYKSRMNKTYCKGARRSQRSSKENFIPCSEPVKGTGALRDCKNVLDVSCRKTGLKLEKAFLEVNRPQIHKLDPSWKERKVTPSKYSSLIYFDSSATYNLDEENRFRTLKWLISPVKIVSRPTIRQGHGENRQREIEIRFDQLHREYCLSPRNQPRRMCLPDSWAMNMYRGGPASPGGLQGLETRRLSLPSSKAKAKSLSEAFENLGKRSLEAGRCLPKSDSSSSLPKTNPTHSATRPQQTSDLHVQGNSSGIFRKSVSPSKTLSVPDKEVPGHGRNRYDEIKEEFDKLHQKYCLKSPGQMTVPLCIGVSTDKASMEVRYQTEGFLGKLNPDPHFQGFQKLPSSPLGCRKSLLGSTAIEAPSSTCVARAITRDGTRDHQFPAKRPRLSEPQGSGRQGNSLGASDGVDNTVRPGDQGSSSQPNSEERGENTSYRMEEKSDFMLEKLETKSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
83
Pig
80
Sheep
56
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NCD3 As Substrate

Site PTM Type Enzyme
K21 Ubiquitination
S25 Phosphorylation
K39 Ubiquitination
S82 Phosphorylation
S83 Phosphorylation
K85 Ubiquitination
S116 Phosphorylation
T122 Phosphorylation
S123 Phosphorylation
S128 Phosphorylation
S140 Phosphorylation
Y163 Phosphorylation
T178 Phosphorylation
S182 Phosphorylation
S185 Phosphorylation
Y193 Phosphorylation
S198 Phosphorylation
S201 Phosphorylation
K207 Ubiquitination
S210 Phosphorylation
S211 Phosphorylation
K278 Ubiquitination
K286 Ubiquitination
S303 Phosphorylation
K321 Ubiquitination
S328 Phosphorylation
K332 Ubiquitination
K341 Ubiquitination
S347 Phosphorylation
K354 Ubiquitination
K357 Ubiquitination
K369 Ubiquitination
K375 Ubiquitination
T380 Phosphorylation
S382 Phosphorylation
K408 Sumoylation
K408 Ubiquitination
S412 Phosphorylation
K415 Ubiquitination
T421 Phosphorylation
Y445 Phosphorylation
S448 Phosphorylation
S473 Phosphorylation
T482 Phosphorylation
S486 Phosphorylation P31749 (AKT1)
S489 Phosphorylation
S490 Phosphorylation
K495 Methylation
K495 Sumoylation
K495 Ubiquitination
S496 Phosphorylation
K506 Methylation
K506 Ubiquitination
S508 Phosphorylation
K517 Sumoylation
K517 Ubiquitination
S520 Phosphorylation
K526 Ubiquitination
S555 Phosphorylation
S557 Phosphorylation
S559 Phosphorylation
K567 Ubiquitination
K581 Sumoylation
K581 Ubiquitination
K586 Ubiquitination
K590 Ubiquitination
K594 Ubiquitination
S595 Phosphorylation
T600 Phosphorylation
K611 Ubiquitination
K626 Ubiquitination
K638 Ubiquitination
S641 Phosphorylation
S642 Phosphorylation
K648 Sumoylation
K648 Ubiquitination
S649 Phosphorylation
T654 Phosphorylation
K681 Ubiquitination
S686 Phosphorylation
K735 Sumoylation
S736 Phosphorylation
K742 Sumoylation
S747 Phosphorylation

Research Backgrounds

Function:

Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.

Subcellular Location:

Nucleus>Nucleolus. Chromosome>Centromere.
Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

According to highly expressed in the thymus with lower levels in the placenta, small intestine, liver, skeletal muscle, and colon. According to highly expressed in testis, and at a relatively lower level in thymus and bone marrow. Significantly overexpressed in many lung cancer samples, compared with normal lung.

Subunit Structure:

Interacts with CENPA (via CATD domain); the interaction is direct and specific for CENPA since it does not interact with H3.1- or H3.3-containing nucleosomes. Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1. Interacts with 14-3-3 family members in a phosphorylation-dependent manner. Interacts with MSH5 and NBN.

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