FAT1 Antibody - #DF8943

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Product: FAT1 Antibody
Catalog: DF8943
Description: Rabbit polyclonal antibody to FAT1
Application: WB IHC
Reactivity: Human, Rat, Monkey
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 7 kDa; 506kD(Calculated).
Uniprot: Q14517
RRID: AB_2842139

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat,Monkey
Prediction:
Bovine(88%), Horse(90%), Sheep(88%), Rabbit(91%), Dog(83%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
FAT1 Antibody detects endogenous levels of total FAT1.
RRID:
AB_2842139
Cite Format: Affinity Biosciences Cat# DF8943, RRID:AB_2842139.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cadherin family member 7; Cadherin family member 7 precursor; Cadherin ME5; Cadherin related tumor suppressor; Cadherin related tumor suppressor homolog; Cadherin related tumor suppressor homolog precursor; Cadherin related tumor suppressor homolog precursor (FAT protein homolog); Cadherin-related tumor suppressor homolog; CDHF 7; CDHF7; FAT 1; FAT tumor suppressor 1; FAT tumor suppressor homolog 1; FAT1; FAT1_HUMAN; hFat 1; hFAT; hFat1; Homolog of Drosophila FAT protein; Homolog of Drosophila FAT protein precursor; Homolog of Drosophila tumor suppressor FAT precursor; ME 5; ME5; nuclear form; Protein fat homolog; Protocadherin Fat 1;

Immunogens

Immunogen:
Uniprot:

Q14517(FAT1_HUMAN) >>Visit HPA database.

Gene(ID):
FAT1(2195)
Expression:
Q14517 FAT1_HUMAN:

Expressed in many epithelial and some endothelial and smooth muscle cells.

Sequence:
MGRHLALLLLLLLLFQHFGDSDGSQRLEQTPLQFTHLEYNVTVQENSAAKTYVGHPVKMGVYITHPAWEVRYKIVSGDSENLFKAEEYILGDFCFLRIRTKGGNTAILNREVKDHYTLIVKALEKNTNVEARTKVRVQVLDTNDLRPLFSPTSYSVSLPENTAIRTSIARVSATDADIGTNGEFYYSFKDRTDMFAIHPTSGVIVLTGRLDYLETKLYEMEILAADRGMKLYGSSGISSMAKLTVHIEQANECAPVITAVTLSPSELDRDPAYAIVTVDDCDQGANGDIASLSIVAGDLLQQFRTVRSFPGSKEYKVKAIGGIDWDSHPFGYNLTLQAKDKGTPPQFSSVKVIHVTSPQFKAGPVKFEKDVYRAEISEFAPPNTPVVMVKAIPAYSHLRYVFKSTPGKAKFSLNYNTGLISILEPVKRQQAAHFELEVTTSDRKASTKVLVKVLGANSNPPEFTQTAYKAAFDENVPIGTTVMSLSAVDPDEGENGYVTYSIANLNHVPFAIDHFTGAVSTSENLDYELMPRVYTLRIRASDWGLPYRREVEVLATITLNNLNDNTPLFEKINCEGTIPRDLGVGEQITTVSAIDADELQLVQYQIEAGNELDFFSLNPNSGVLSLKRSLMDGLGAKVSFHSLRITATDGENFATPLYINITVAASHKLVNLQCEETGVAKMLAEKLLQANKLHNQGEVEDIFFDSHSVNAHIPQFRSTLPTGIQVKENQPVGSSVIFMNSTDLDTGFNGKLVYAVSGGNEDSCFMIDMETGMLKILSPLDRETTDKYTLNITVYDLGIPQKAAWRLLHVVVVDANDNPPEFLQESYFVEVSEDKEVHSEIIQVEATDKDLGPNGHVTYSIVTDTDTFSIDSVTGVVNIARPLDRELQHEHSLKIEARDQAREEPQLFSTVVVKVSLEDVNDNPPTFIPPNYRVKVREDLPEGTVIMWLEAHDPDLGQSGQVRYSLLDHGEGNFDVDKLSGAVRIVQQLDFEKKQVYNLTVRAKDKGKPVSLSSTCYVEVEVVDVNENLHPPVFSSFVEKGTVKEDAPVGSLVMTVSAHDEDARRDGEIRYSIRDGSGVGVFKIGEETGVIETSDRLDRESTSHYWLTVFATDQGVVPLSSFIEIYIEVEDVNDNAPQTSEPVYYPEIMENSPKDVSVVQIEAFDPDSSSNDKLMYKITSGNPQGFFSIHPKTGLITTTSRKLDREQQDEHILEVTVTDNGSPPKSTIARVIVKILDENDNKPQFLQKFYKIRLPEREKPDRERNARREPLYHVIATDKDEGPNAEISYSIEDGNEHGKFFIEPKTGVVSSKRFSAAGEYDILSIKAVDNGRPQKSSTTRLHIEWISKPKPSLEPISFEESFFTFTVMESDPVAHMIGVISVEPPGIPLWFDITGGNYDSHFDVDKGTGTIIVAKPLDAEQKSNYNLTVEATDGTTTILTQVFIKVIDTNDHRPQFSTSKYEVVIPEDTAPETEILQISAVDQDEKNKLIYTLQSSRDPLSLKKFRLDPATGSLYTSEKLDHEAVHQHTLTVMVRDQDVPVKRNFARIVVNVSDTNDHAPWFTASSYKGRVYESAAVGSVVLQVTALDKDKGKNAEVLYSIESGNIGNSFMIDPVLGSIKTAKELDRSNQAEYDLMVKATDKGSPPMSEITSVRIFVTIADNASPKFTSKEYSVELSETVSIGSFVGMVTAHSQSSVVYEIKDGNTGDAFDINPHSGTIITQKALDFETLPIYTLIIQGTNMAGLSTNTTVLVHLQDENDNAPVFMQAEYTGLISESASINSVVLTDRNVPLVIRAADADKDSNALLVYHIVEPSVHTYFAIDSSTGAIHTVLSLDYEETSIFHFTVQVHDMGTPRLFAEYAANVTVHVIDINDCPPVFAKPLYEASLLLPTYKGVKVITVNATDADSSAFSQLIYSITEGNIGEKFSMDYKTGALTVQNTTQLRSRYELTVRASDGRFAGLTSVKINVKESKESHLKFTQDVYSAVVKENSTEAETLAVITAIGNPINEPLFYHILNPDRRFKISRTSGVLSTTGTPFDREQQEAFDVVVEVTEEHKPSAVAHVVVKVIVEDQNDNAPVFVNLPYYAVVKVDTEVGHVIRYVTAVDRDSGRNGEVHYYLKEHHEHFQIGPLGEISLKKQFELDTLNKEYLVTVVAKDGGNPAFSAEVIVPITVMNKAMPVFEKPFYSAEIAESIQVHSPVVHVQANSPEGLKVFYSITDGDPFSQFTINFNTGVINVIAPLDFEAHPAYKLSIRATDSLTGAHAEVFVDIIVDDINDNPPVFAQQSYAVTLSEASVIGTSVVQVRATDSDSEPNRGISYQMFGNHSKSHDHFHVDSSTGLISLLRTLDYEQSRQHTIFVRAVDGGMPTLSSDVIVTVDVTDLNDNPPLFEQQIYEARISEHAPHGHFVTCVKAYDADSSDIDKLQYSILSGNDHKHFVIDSATGIITLSNLHRHALKPFYSLNLSVSDGVFRSSTQVHVTVIGGNLHSPAFLQNEYEVELAENAPLHTLVMEVKTTDGDSGIYGHVTYHIVNDFAKDRFYINERGQIFTLEKLDRETPAEKVISVRLMAKDAGGKVAFCTVNVILTDDNDNAPQFRATKYEVNIGSSAAKGTSVVKVLASDADEGSNADITYAIEADSESVKENLEINKLSGVITTKESLIGLENEFFTFFVRAVDNGSPSKESVVLVYVKILPPEMQLPKFSEPFYTFTVSEDVPIGTEIDLIRAEHSGTVLYSLVKGNTPESNRDESFVIDRQSGRLKLEKSLDHETTKWYQFSILARCTQDDHEMVASVDVSIQVKDANDNSPVFESSPYEAFIVENLPGGSRVIQIRASDADSGTNGQVMYSLDQSQSVEVIESFAINMETGWITTLKELDHEKRDNYQIKVVASDHGEKIQLSSTAIVDVTVTDVNDSPPRFTAEIYKGTVSEDDPQGGVIAILSTTDADSEEINRQVTYFITGGDPLGQFAVETIQNEWKVYVKKPLDREKRDNYLLTITATDGTFSSKAIVEVKVLDANDNSPVCEKTLYSDTIPEDVLPGKLIMQISATDADIRSNAEITYTLLGSGAEKFKLNPDTGELKTSTPLDREEQAVYHLLVRATDGGGRFCQASIVLTLEDVNDNAPEFSADPYAITVFENTEPGTLLTRVQATDADAGLNRKILYSLIDSADGQFSINELSGIIQLEKPLDRELQAVYTLSLKAVDQGLPRRLTATGTVIVSVLDINDNPPVFEYREYGATVSEDILVGTEVLQVYAASRDIEANAEITYSIISGNEHGKFSIDSKTGAVFIIENLDYESSHEYYLTVEATDGGTPSLSDVATVNVNVTDINDNTPVFSQDTYTTVISEDAVLEQSVITVMADDADGPSNSHIHYSIIDGNQGSSFTIDPVRGEVKVTKLLDRETISGYTLTVQASDNGSPPRVNTTTVNIDVSDVNDNAPVFSRGNYSVIIQENKPVGFSVLQLVVTDEDSSHNGPPFFFTIVTGNDEKAFEVNPQGVLLTSSAIKRKEKDHYLLQVKVADNGKPQLSSLTYIDIRVIEESIYPPAILPLEIFITSSGEEYSGGVIGKIHATDQDVYDTLTYSLDPQMDNLFSVSSTGGKLIAHKKLDIGQYLLNVSVTDGKFTTVADITVHIRQVTQEMLNHTIAIRFANLTPEEFVGDYWRNFQRALRNILGVRRNDIQIVSLQSSEPHPHLDVLLFVEKPGSAQISTKQLLHKINSSVTDIEEIIGVRILNVFQKLCAGLDCPWKFCDEKVSVDESVMSTHSTARLSFVTPRHHRAAVCLCKEGRCPPVHHGCEDDPCPEGSECVSDPWEEKHTCVCPSGRFGQCPGSSSMTLTGNSYVKYRLTENENKLEMKLTMRLRTYSTHAVVMYARGTDYSILEIHHGRLQYKFDCGSGPGIVSVQSIQVNDGQWHAVALEVNGNYARLVLDQVHTASGTAPGTLKTLNLDNYVFFGGHIRQQGTRHGRSPQVGNGFRGCMDSIYLNGQELPLNSKPRSYAHIEESVDVSPGCFLTATEDCASNPCQNGGVCNPSPAGGYYCKCSALYIGTHCEISVNPCSSKPCLYGGTCVVDNGGFVCQCRGLYTGQRCQLSPYCKDEPCKNGGTCFDSLDGAVCQCDSGFRGERCQSDIDECSGNPCLHGALCENTHGSYHCNCSHEYRGRHCEDAAPNQYVSTPWNIGLAEGIGIVVFVAGIFLLVVVFVLCRKMISRKKKHQAEPKDKHLGPATAFLQRPYFDSKLNKNIYSDIPPQVPVRPISYTPSIPSDSRNNLDRNSFEGSAIPEHPEFSTFNPESVHGHRKAVAVCSVAPNLPPPPPSNSPSDSDSIQKPSWDFDYDTKVVDLDPCLSKKPLEEKPSQPYSARESLSEVQSLSSFQSESCDDNGYHWDTSDWMPSVPLPDIQEFPNYEVIDEQTPLYSADPNAIDTDYYPGGYDIESDFPPPPEDFPAADELPPLPPEFSNQFESIHPPRDMPAAGSLGSSSRNRQRFNLNQYLPNFYPLDMSEPQTKGTGENSTCREPHAPYPPGYQRHFEAPAVESMPMSVYASTASCSDVSACCEVESEVMMSDYESGDDGHFEEVTIPPLDSQQHTEV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Horse
90
Bovine
88
Sheep
88
Chicken
86
Dog
83
Pig
73
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14517 As Substrate

Site PTM Type Enzyme
K73 Ubiquitination
K101 Ubiquitination
K121 Ubiquitination
K125 Ubiquitination
S150 Phosphorylation
T152 Phosphorylation
S153 Phosphorylation
S155 Phosphorylation
K189 Ubiquitination
T192 Phosphorylation
Y212 Phosphorylation
K216 Ubiquitination
K339 Ubiquitination
K341 Ubiquitination
S357 Phosphorylation
K366 Ubiquitination
K369 Ubiquitination
K390 Ubiquitination
K403 Ubiquitination
K427 Ubiquitination
K452 Ubiquitination
T464 Phosphorylation
Y468 Phosphorylation
Y534 Phosphorylation
T535 Phosphorylation
K571 Ubiquitination
K686 Ubiquitination
K692 Ubiquitination
K727 Ubiquitination
T771 Phosphorylation
S778 Phosphorylation
Y788 Phosphorylation
T789 Phosphorylation
T793 Phosphorylation
Y795 Phosphorylation
K894 Ubiquitination
K935 Ubiquitination
S959 Phosphorylation
K978 Ubiquitination
K993 Ubiquitination
T1000 Phosphorylation
K1083 Ubiquitination
K1173 Ubiquitination
Y1176 Phosphorylation
S1188 O-Glycosylation
K1192 Ubiquitination
K1242 Ubiquitination
K1248 Ubiquitination
T1277 Phosphorylation
K1305 Ubiquitination
T1306 Phosphorylation
K1326 Ubiquitination
K1335 Ubiquitination
K1415 Ubiquitination
K1460 Ubiquitination
K1486 Ubiquitination
S1496 Phosphorylation
K1503 Ubiquitination
K1542 Ubiquitination
K1568 Ubiquitination
K1638 Ubiquitination
K1642 Ubiquitination
K1666 Ubiquitination
K1894 Ubiquitination
K1932 Ubiquitination
T1937 Phosphorylation
K1966 Ubiquitination
K1970 Ubiquitination
K1978 Ubiquitination
T1980 Phosphorylation
S1992 Phosphorylation
T2002 Phosphorylation
Y2086 Phosphorylation
K2138 Ubiquitination
K2139 Ubiquitination
K2424 Ubiquitination
K2553 Ubiquitination
K2600 Ubiquitination
Y2601 Phosphorylation
S2608 Phosphorylation
K2611 Ubiquitination
T2613 Phosphorylation
S2621 Phosphorylation
S2627 Phosphorylation
Y2633 Phosphorylation
K2643 Ubiquitination
K2650 Ubiquitination
K2683 Ubiquitination
T2720 Phosphorylation
K2739 Ubiquitination
K2761 Ubiquitination
K2764 Ubiquitination
K2886 Ubiquitination
K2981 Ubiquitination
K3025 Ubiquitination
T3048 Phosphorylation
K3069 Ubiquitination
K3071 Acetylation
K3071 Ubiquitination
S3082 Phosphorylation
Y3253 Phosphorylation
K3396 Ubiquitination
T3402 Phosphorylation
S3404 Phosphorylation
Y3406 Phosphorylation
T3407 Phosphorylation
S3417 Phosphorylation
N3422 N-Glycosylation
K3504 Ubiquitination
K3522 Ubiquitination
K3603 Acetylation
N3716 N-Glycosylation
K3736 Ubiquitination
K3746 Ubiquitination
K3751 Ubiquitination
S3757 Phosphorylation
S3760 Phosphorylation
K3783 Ubiquitination
K3813 Ubiquitination
Y3842 Phosphorylation
T3845 Phosphorylation
K3850 Ubiquitination
T3861 Phosphorylation
S3863 Phosphorylation
T3940 Phosphorylation
K3942 Ubiquitination
K3992 Ubiquitination
K4094 Ubiquitination
S4207 Phosphorylation
K4219 Ubiquitination
Y4232 Phosphorylation
K4236 Ubiquitination
K4239 Ubiquitination
Y4242 Phosphorylation
S4243 Phosphorylation
S4255 Phosphorylation
S4272 Phosphorylation
S4303 Phosphorylation
S4316 Phosphorylation
S4318 Phosphorylation
S4322 Phosphorylation
S4344 Phosphorylation
K4351 Ubiquitination
S4353 Phosphorylation
Y4356 Phosphorylation
S4357 Phosphorylation
S4473 Phosphorylation
S4476 Phosphorylation
S4477 Phosphorylation
Y4489 Phosphorylation
Y4494 Phosphorylation
Y4519 Phosphorylation
Y4523 Phosphorylation

Research Backgrounds

Function:

Plays an essential role for cellular polarization, directed cell migration and modulating cell-cell contact.

PTMs:

Undergoes proteolytic cleavage. The extracellular domain is cleaved off and the cytoplasmic domain (about 400 AA) shuttles to the nucleus.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Nucleus. Cytoplasm>Perinuclear region.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in many epithelial and some endothelial and smooth muscle cells.

Subunit Structure:

Interacts (via the C-terminus 4300-4400 AA) with ATN1. Interacts with RERE. Interacts (via EVH1 domains) with ENAH (By similarity). Interacts (via cytoplasmic domain) with CTNNB1 (By similarity).

Family&Domains:

A PTB-like motif (DNXYH sequence) is required for the targeting to the leading edge. This motif represents a minimal protein-protein interaction core motif that is not regulated by tyrosine phosphorylation (By similarity).

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