IFIT2 Antibody - #DF8962

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Product: IFIT2 Antibody
Catalog: DF8962
Description: Rabbit polyclonal antibody to IFIT2
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig
Mol.Wt.: 55kDa; 55kD(Calculated).
Uniprot: P09913
RRID: AB_2842158

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(80%)
Clonality:
Polyclonal
Specificity:
IFIT2 Antibody detects endogenous levels of total IFIT2.
RRID:
AB_2842158
Cite Format: Affinity Biosciences Cat# DF8962, RRID:AB_2842158.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CIG-42; cig42; G10P2; GARG 39; Glucocorticoid attenuated response gene 39; IFI 54; IFI 54K; IFI-54K; IFI54; IFIT 2; IFIT-2; IFIT2; IFIT2_HUMAN; Interferon induced 54 kDa protein; Interferon induced protein 54; Interferon induced protein with tetratricopeptide repeats 2; Interferon, alpha inducible protein (MW 54kD); Interferon-induced 54 kDa protein; Interferon-induced protein with tetratricopeptide repeats 2; ISG 54 K; ISG 54K; ISG-54 K; ISG54;

Immunogens

Immunogen:
Uniprot:

P09913(IFIT2_HUMAN) >>Visit HPA database.

Gene(ID):
IFIT2(3433)
Sequence:
MSENNKNSLESSLRQLKCHFTWNLMEGENSLDDFEDKVFYRTEFQNREFKATMCNLLAYLKHLKGQNEAALECLRKAEELIQQEHADQAEIRSLVTWGNYAWVYYHMGRLSDVQIYVDKVKHVCEKFSSPYRIESPELDCEEGWTRLKCGGNQNERAKVCFEKALEKKPKNPEFTSGLAIASYRLDNWPPSQNAIDPLRQAIRLNPDNQYLKVLLALKLHKMREEGEEEGEGEKLVEEALEKAPGVTDVLRSAAKFYRRKDEPDKAIELLKKALEYIPNNAYLHCQIGCCYRAKVFQVMNLRENGMYGKRKLLELIGHAVAHLKKADEANDNLFRVCSILASLHALADQYEDAEYYFQKEFSKELTPVAKQLLHLRYGNFQLYQMKCEDKAIHHFIEGVKINQKSREKEKMKDKLQKIAKMRLSKNGADSEALHVLAFLQELNEKMQQADEDSERGLESGSLIPSASSWNGE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
80
Bovine
78
Dog
60
Sheep
56
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09913 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K6 Ubiquitination
S8 Phosphorylation
K64 Ubiquitination
K126 Ubiquitination
K148 Ubiquitination
K158 Ubiquitination
K163 Ubiquitination
S191 Phosphorylation
K212 Ubiquitination
K218 Ubiquitination
K221 Ubiquitination
K234 Ubiquitination
K242 Ubiquitination
K255 Ubiquitination
K265 Ubiquitination
K272 Ubiquitination
K294 Ubiquitination
K309 Methylation
K325 Ubiquitination
K363 Ubiquitination
K370 Ubiquitination
Y383 Phosphorylation
K390 Ubiquitination
K400 Ubiquitination
K417 Acetylation
K420 Acetylation
S424 Phosphorylation
K425 Ubiquitination
K445 Ubiquitination
S461 Phosphorylation

Research Backgrounds

Function:

IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.

Subcellular Location:

Cytoplasm. Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Domain-swapped homodimer. Component of an interferon-dependent multiprotein complex, at least composed of IFIT1, IFIT2 and IFIT3. Interacts with IFIT1 and IFIT3. Interacts with STING1/MITA and disrupts its interaction with MAVS or TBK1. Interacts with EIF3E and EIF3C.

Family&Domains:

The C-terminal part folds into a super-helical structure and has an extensively positively-charged nucleotide-binding channel on its inner surface.

Belongs to the IFIT family.

References

1). METTL3 promotes intrahepatic cholangiocarcinoma progression by regulating IFIT2 expression in an m6A-YTHDF2-dependent manner. Oncogene, 2022 (PubMed: 35094011) [IF=6.9]

Application: WB    Species: Human    Sample: HuCC-T1 and HCCC-9810 cells

Fig. 6 METTL3 accelerates the ICC progression by downregulating IFIT2 expression. A The mRNA level of IFIT2 after IFIT2 silencing in HuCC-T1 and HCCC-9810 cells was confirmed by RT-qPCR. B The protein level of IFIT2 after IFIT2 silencing in HuCC-T1 and HCCC-9810 cells was confirmed by western blotting. C Cell growth curve of HuCC-T1 and HCCC-9810 cells transfected with IFIT2 siRNA or Control. D Cell migration ability and cell invasion ability after IFIT2 silencing in HuCC-T1 and HCCC-9810 cells. E The mRNA level of METTL3 and IFIT2 after METTL3 or /and IFIT2 silencing in HuCC-T1 and HCCC-9810 cells was confirmed by RT-qPCR. F The protein level of METTL3 and IFIT2 after METTL3 or /and IFIT2 silencing in HuCC-T1 and HCCC-9810 cells was confirmed by western blotting. G Cell growth curve of HuCC-T1 and HCCC-9810 cells after METTL3 or /and IFIT2 silencing. H Cell migration ability and cell invasion ability in HuCC-T1 and HCCC-9810 cells after METTL3 or /and IFIT2 silencing. The results are presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, according to Student’s t test.

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