IL31 Antibody - #DF8986
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Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
IL31 Antibody detects endogenous levels of total IL31.
RRID:
AB_2842182
Cite Format: Affinity Biosciences Cat# DF8986, RRID:AB_2842182.
Cite Format: Affinity Biosciences Cat# DF8986, RRID:AB_2842182.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
IL 31; IL-31; IL31; IL31_HUMAN; Interleukin 31; Interleukin-31;
Immunogens
Immunogen:
A synthesized peptide derived from human IL31, corresponding to a region within the internal amino acids.
Uniprot:
Gene(ID):
Expression:
Q6EBC2 IL31_HUMAN:
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
Sequence:
- Q6EBC2 IL31_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASHSGPSTSVLFLFCCLGGWLASHTLPVRLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTLPCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTISQQFSECMDLALKSLTSGAQQATT
Research Backgrounds
Function:
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. May function in skin immunity. Enhances myeloid progenitor cell survival in vitro (By similarity). Induces RETNLA and serum amyloid A protein expression in macrophages (By similarity).
Subcellular Location:
Secreted.
Tissue Specificity:
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
References
1). TIA1-Mediated Stress Granules Promote the Neuroinflammation and Demyelination in Experimental Autoimmune Encephalomyelitis through Upregulating IL-31RA Signaling. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025
(PubMed: 39804990)
[IF=15.1]
Application: WB Species: Mouse Sample: spinal cord tissue
Figure 6 IL-31RA was reduced in spinal cord neurons in Tia1Nestin-CKO EAE mice. A) GO analysis of RNA-seq in spinal cord tissue of Tia1f/f EAE mice and Tia1Nestin-CKO EAE mice (n = 4 mice per group). B) The heatmap of differential mRNAs in spinal cords of Tia1f/f EAE mice and Tia1Nestin-CKO EAE mice (n = 4 mice per group) based on RNA-seq. C,D) Analysis of qPCR showed the relative mRNA level of IL-31RA (C, n = 6 mice per group) and IL-31 (D, n = 4 mice per group) in spinal cords of Tia1f/f EAE mice and Tia1Nestin-CKO EAE mice (n = 6 mice per group). E) Western blot analysis of the IL-31RA and IL-31 expression in spinal cords of Tia1f/f mice and Tia1Nestin-CKO mice, Tia1f/f EAE mice and Tia1Nestin-CKO EAE mice. F,G) Quantitative analysis of the relative IL-31RA levels (F, normalized to Tia1f/f mice, n = 4 blots from 4 mice per group, two-way ANOVA), and the relative IL-31 levels (G, normalized to Tia1f/f mice, n = 5 blots from 5 mice per group, two-way ANOVA) as shown in (E). H,I) Double immunostaining of IL-31RA (red) and NeuN (green) (H), and IL-31 (green) and F4/80 (red) (I) in spinal cords of Tia1f/f EAE mice and Tia1Nestin-CKO EAE mice. J) Quantitative analysis of the relative IL-31RA expression as shown in (H) (n = 4 sections from 4 mice per group). K,L) Quantitative analysis of the relative F4/80 expression (K, n = 4 sections from 4 mice per group) and IL-31 expression (L, n = 4 sections from 4 mice per group) as shown in (I). M) Western blot analysis of IL-31 expression in CSF of normal person and MS patients. Scale bars, 20 µm. Data were mean ± SEM. Student's t-test unless otherwise indicated, n.s., not significant. *p < 0.05, **p < 0.01, ***p < 0.001.
Application: IF/ICC Species: Mouse Sample: spinal cord tissue
Figure 6 IL-31RA was reduced in spinal cord neurons in Tia1Nestin-CKO EAE mice. A) GO analysis of RNA-seq in spinal cord tissue of Tia1f/f EAE mice and Tia1Nestin-CKO EAE mice (n = 4 mice per group). B) The heatmap of differential mRNAs in spinal cords of Tia1f/f EAE mice and Tia1Nestin-CKO EAE mice (n = 4 mice per group) based on RNA-seq. C,D) Analysis of qPCR showed the relative mRNA level of IL-31RA (C, n = 6 mice per group) and IL-31 (D, n = 4 mice per group) in spinal cords of Tia1f/f EAE mice and Tia1Nestin-CKO EAE mice (n = 6 mice per group). E) Western blot analysis of the IL-31RA and IL-31 expression in spinal cords of Tia1f/f mice and Tia1Nestin-CKO mice, Tia1f/f EAE mice and Tia1Nestin-CKO EAE mice. F,G) Quantitative analysis of the relative IL-31RA levels (F, normalized to Tia1f/f mice, n = 4 blots from 4 mice per group, two-way ANOVA), and the relative IL-31 levels (G, normalized to Tia1f/f mice, n = 5 blots from 5 mice per group, two-way ANOVA) as shown in (E). H,I) Double immunostaining of IL-31RA (red) and NeuN (green) (H), and IL-31 (green) and F4/80 (red) (I) in spinal cords of Tia1f/f EAE mice and Tia1Nestin-CKO EAE mice. J) Quantitative analysis of the relative IL-31RA expression as shown in (H) (n = 4 sections from 4 mice per group). K,L) Quantitative analysis of the relative F4/80 expression (K, n = 4 sections from 4 mice per group) and IL-31 expression (L, n = 4 sections from 4 mice per group) as shown in (I). M) Western blot analysis of IL-31 expression in CSF of normal person and MS patients. Scale bars, 20 µm. Data were mean ± SEM. Student's t-test unless otherwise indicated, n.s., not significant. *p < 0.05, **p < 0.01, ***p < 0.001.
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