INSIG2 Antibody - #DF8990

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Product: INSIG2 Antibody
Catalog: DF8990
Description: Rabbit polyclonal antibody to INSIG2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 25 kDa; 25kD(Calculated).
Uniprot: Q9Y5U4
RRID: AB_2842186

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(100%), Dog(90%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
INSIG2 Antibody detects endogenous levels of total INSIG2.
RRID:
AB_2842186
Cite Format: Affinity Biosciences Cat# DF8990, RRID:AB_2842186.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hypothetical protein INSIG2; INSIG 2; INSIG 2 membrane protein; INSIG2 membrane protein; Insulin induced gene 2; Insulin induced gene 2 protein; Insulin induced protein 2; MGC26273;

Immunogens

Immunogen:
Uniprot:

Q9Y5U4(INSI2_HUMAN) >>Visit HPA database.

Gene(ID):
INSIG2(51141)
Sequence:
MAEGETESPGPKKCGPYISSVTSQSVNLMIRGVVLFFIGVFLALVLNLLQIQRNVTLFPPDVIASIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNIQLSLTLAALSIGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYECKVIAEKSHQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Horse
90
Bovine
90
Sheep
90
Dog
90
Chicken
80
Zebrafish
60
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5U4 As Substrate

Site PTM Type Enzyme
K100 Ubiquitination
K102 Ubiquitination
K221 Ubiquitination

Research Backgrounds

Function:

Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139. Interacts with RNF145.

Family&Domains:

Belongs to the INSIG family.

References

1). Ginsenosides From Panax ginseng Improves Hepatic Lipid Metabolism Disorders in HFD-Fed Rats by Regulating Gut Microbiota and Cholesterol Metabolism Signaling Pathways. Phytotherapy research : PTR, 2024 (PubMed: 39660634) [IF=6.1]

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