ITFG2 Antibody | Affinity Biosciences

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ITFG2 Antibody - Western blot analysis ITFG2 using NIH-3T3 whole cell lysates.

Product:	ITFG2 Antibody
Catalog:	DF8997
Description:	Rabbit polyclonal antibody to ITFG2
Application:	WB
Reactivity:	Human, Mouse
Mol.Wt.:	49 kDa; 49kD(Calculated).
Uniprot:	Q969R8
RRID:	AB_2842193

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

ITFG2 Antibody detects endogenous levels of total ITFG2.

RRID:

AB_2842193
Cite Format: Affinity Biosciences Cat# DF8997, RRID:AB_2842193.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Integrin alpha FG GAP repeat containing 2; Integrin alpha FG GAP repeat containing protein 2; Integrin-alpha FG-GAP repeat-containing protein 2; ITFG 2; ITFG2; ITFG2_HUMAN; MDS 028; MDS028; OTTHUMP00000161190; RGD1311944; Uncharacterized hematopoietic stem/progenitor cells protein MDS028;

Immunogens

Immunogen:

Uniprot:

Q969R8(ITFG2_HUMAN) >>Visit HPA database.

Gene(ID):

ITFG2(55846)

Sequence:

Q969R8 ITFG2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRSVSYVQRVALEFSGSLFPHAICLGDVDNDTLNELVVGDTSGKVSVYKNDDSRPWLTCSCQGMLTCVGVGDVCNKGKNLLVAVSAEGWFHLFDLTPAKVLDASGHHETLIGEEQRPVFKQHIPANTKVMLISDIDGDGCRELVVGYTDRVVRAFRWEELGEGPEHLTGQLVSLKKWMLEGQVDSLSVTLGPLGLPELMVSQPGCAYAILLCTWKKDTGSPPASEGPTDGSRETPAARDVVLHQTSGRIHNKNVSTHLIGNIKQGHGTESSGSGLFALCTLDGTLKLMEEMEEADKLLWSVQVDHQLFALEKLDVTGNGHEEVVACAWDGQTYIIDHNRTVVRFQVDENIRAFCAGLYACKEGRNSPCLVYVTFNQKIYVYWEVQLERMESTNLVKLLETKPEYHSLLQELGVDPDDLPVTRALLHQTLYHPDQPPQCAPSSLQDPT

PTMs - Q969R8 As Substrate

Q969R8

Site	PTM Type	Source
Y48	Phosphorylation	Uniprot
K49	Ubiquitination	Uniprot
S53	Phosphorylation	Uniprot
S104	Phosphorylation	Uniprot
K120	Ubiquitination	Uniprot
Y207	Phosphorylation	Uniprot
T218	Phosphorylation	Uniprot
S220	Phosphorylation	Uniprot
T234	Phosphorylation	Uniprot
T245	Phosphorylation	Uniprot
S246	Phosphorylation	Uniprot
K252	Ubiquitination	Uniprot
K361	Ubiquitination	Uniprot
K401	Ubiquitination	Uniprot

Research Backgrounds

Q969R8_ITFG2_HUMAN

Function:

As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.

Subcellular Location:

Lysosome membrane.
Note: Localization to lysosomes is amino acid-independent.

Subunit Structure:

Part of the KICSTOR complex composed of KPTN, ITFG2, C12orf66 and SZT2. SZT2 probably serves as a link between the other three proteins in the KICSTOR complex and may mediate the direct interaction with the GATOR complex via GATOR1. The KICSTOR complex interacts directly with the GATOR1 complex and most probably indirectly with the GATOR2 complexe in an amino acid-independent manner.

Restrictive clause

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ITFG2 Antibody - #DF8997