KDM5A Antibody - #DF8998

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Product: KDM5A Antibody
Catalog: DF8998
Description: Rabbit polyclonal antibody to KDM5A
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Dog, Chicken
Mol.Wt.: 192kDa; 192kD(Calculated).
Uniprot: P29375
RRID: AB_2842194

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Horse(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
KDM5A Antibody detects endogenous levels of total KDM5A.
RRID:
AB_2842194
Cite Format: Affinity Biosciences Cat# DF8998, RRID:AB_2842194.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Histone demethylase JARID1A; JARID1A; Jumonji/ARID domain containing protein 1A; Jumonji/ARID domain-containing protein 1A; Kdm5a; KDM5A_HUMAN; Lysine-specific demethylase 5A; RBBP-2; RBBP2; RBP2; Retinoblastoma binding protein 2; Retinoblastoma-binding protein 2;

Immunogens

Immunogen:
Uniprot:

P29375(KDM5A_HUMAN) >>Visit HPA database.

Gene(ID):
KDM5A(5927)
Sequence:
MAGVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPLAEKTGICKIRPPKDWQPPFACEVKSFRFTPRVQRLNELEAMTRVRLDFLDQLAKFWELQGSTLKIPVVERKILDLYALSKIVASKGGFEMVTKEKKWSKVGSRLGYLPGKGTGSLLKSHYERILYPYELFQSGVSLMGVQMPNLDLKEKVEPEVLSTDTQTSPEPGTRMNILPKRTRRVKTQSESGDVSRNTELKKLQIFGAGPKVVGLAMGTKDKEDEVTRRRKVTNRSDAFNMQMRQRKGTLSVNFVDLYVCMFCGRGNNEDKLLLCDGCDDSYHTFCLIPPLPDVPKGDWRCPKCVAEECSKPREAFGFEQAVREYTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVIVEYGADISSKDFGSGFPVKDGRRKILPEEEEYALSGWNLNNMPVLEQSVLAHINVDISGMKVPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIMNPNVLMEHGVPVYRTNQCAGEFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVNHYRRLRRHCVFSHEELIFKMAADPECLDVGLAAMVCKELTLMTEEETRLRESVVQMGVLMSEEEVFELVPDDERQCSACRTTCFLSALTCSCNPERLVCLYHPTDLCPCPMQKKCLRYRYPLEDLPSLLYGVKVRAQSYDTWVSRVTEALSANFNHKKDLIELRVMLEDAEDRKYPENDLFRKLRDAVKEAETCASVAQLLLSKKQKHRQSPDSGRTRTKLTVEELKAFVQQLFSLPCVISQARQVKNLLDDVEEFHERAQEAMMDETPDSSKLQMLIDMGSSLYVELPELPRLKQELQQARWLDEVRLTLSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLTVSERWEEKAKVCLQARPRHSVASLESIVNEAKNIPAFLPNVLSLKEALQKAREWTAKVEAIQSGSNYAYLEQLESLSAKGRPIPVRLEALPQVESQVAAARAWRERTGRTFLKKNSSHTLLQVLSPRTDIGVYGSGKNRRKKVKELIEKEKEKDLDLEPLSDLEEGLEETRDTAMVVAVFKEREQKEIEAMHSLRAANLAKMTMVDRIEEVKFCICRKTASGFMLQCELCKDWFHNSCVPLPKSSSQKKGSSWQAKEVKFLCPLCMRSRRPRLETILSLLVSLQKLPVRLPEGEALQCLTERAMSWQDRARQALATDELSSALAKLSVLSQRMVEQAAREKTEKIISAELQKAAANPDLQGHLPSFQQSAFNRVVSSVSSSPRQTMDYDDEETDSDEDIRETYGYDMKDTASVKSSSSLEPNLFCDEEIPIKSEEVVTHMWTAPSFCAEHAYSSASKSCSQGSSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELMMVGDLLEVSLDETQHIWRILQATHPPSEDRFLHIMEDDSMEEKPLKVKGKDSSEKKRKRKLEKVEQLFGEGKQKSKELKKMDKPRKKKLKLGADKSKELNKLAKKLAKEEERKKKKEKAAAAKVELVKESTEKKREKKVLDIPSKYDWSGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMAENEDYICINCAKKQGPVSPGPAPPPSFIMSYKLPMEDLKETS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Chicken
100
Pig
91
Bovine
73
Sheep
73
Rabbit
73
Zebrafish
64
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29375 As Substrate

Site PTM Type Enzyme
K45 Ubiquitination
T46 Phosphorylation
K50 Ubiquitination
K106 Ubiquitination
K113 Ubiquitination
K127 Ubiquitination
T134 Phosphorylation
K135 Ubiquitination
K152 Ubiquitination
K159 Ubiquitination
S160 Phosphorylation
Y162 Phosphorylation
K191 Sumoylation
K191 Ubiquitination
T203 Phosphorylation
S204 Phosphorylation
K222 Sumoylation
K222 Ubiquitination
S225 Phosphorylation
K238 Ubiquitination
K247 Ubiquitination
T285 Phosphorylation
S287 Phosphorylation
K339 Ubiquitination
K347 Ubiquitination
K392 Ubiquitination
S399 Phosphorylation
K425 Ubiquitination
K733 Ubiquitination
K757 Ubiquitination
K774 Ubiquitination
K789 Ubiquitination
K804 Ubiquitination
K820 Ubiquitination
K847 Ubiquitination
K895 Sumoylation
K895 Ubiquitination
T918 Phosphorylation
K924 Acetylation
K924 Ubiquitination
K960 Ubiquitination
S970 Phosphorylation
S973 Phosphorylation
K982 Ubiquitination
K995 Ubiquitination
K1007 Ubiquitination
K1029 Ubiquitination
S1066 Phosphorylation
T1069 Phosphorylation
S1075 Phosphorylation
K1087 Ubiquitination
K1094 Ubiquitination
K1103 Ubiquitination
S1111 Phosphorylation
K1136 Ubiquitination
S1143 Phosphorylation
K1151 Ubiquitination
K1206 Ubiquitination
K1209 Ubiquitination
T1225 Phosphorylation
S1255 Phosphorylation
K1275 Ubiquitination
S1280 Phosphorylation
K1294 Ubiquitination
K1302 Ubiquitination
S1327 Phosphorylation
S1329 Phosphorylation
S1330 Phosphorylation
S1331 Phosphorylation
T1343 Phosphorylation
S1345 Phosphorylation
K1364 Ubiquitination
S1365 Phosphorylation
K1382 Sumoylation
S1410 Phosphorylation
S1414 Phosphorylation
T1415 Phosphorylation
S1423 Phosphorylation
S1438 Phosphorylation
S1488 Phosphorylation
K1539 Ubiquitination
S1579 Phosphorylation
S1598 Phosphorylation
S1603 Phosphorylation
S1666 Phosphorylation
S1674 Phosphorylation

Research Backgrounds

Function:

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif. May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (By similarity). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells.

Subcellular Location:

Nucleus>Nucleolus. Nucleus.
Note: Occupies promoters of genes involved in RNA metabolism and mitochondrial function.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SUZ12; the interaction is direct (By similarity). Interacts with the viral protein-binding domain of RB1. Interacts with ESR1, MYC, MYCN and LMO2. Interacts with HDAC1 (By similarity). Interacts with ARNTL/BMAL1 and CLOCK. Interacts (via PHD-type 1 zinc finger) with histone H3 unmodified at 'Lys-4' and (via PHD-type 3 zinc finger) with histone H3 di- and trimethylated at 'Lys-4'.

Family&Domains:

The GSGFP motif is required for the interaction with SUZ12 (By similarity). The ARID domain specifically binds to the CCGCCC motif and is required for the lysine-specific histone demethylase activity (PubMed:18270511). The PHD-type 3 zinc finger is required for the interaction with histone H3 di- and trimethylated at 'Lys-4' (PubMed:19430464).

Belongs to the JARID1 histone demethylase family.

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