Keratin 23 Antibody - #DF9000

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Product: Keratin 23 Antibody
Catalog: DF9000
Description: Rabbit polyclonal antibody to Keratin 23
Application: WB IHC
Cited expt.: WB, IHC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Sheep, Rabbit
Mol.Wt.: 48 kDa; 48kD(Calculated).
Uniprot: Q9C075
RRID: AB_2842196

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(80%), Bovine(83%), Sheep(92%), Rabbit(83%)
Clonality:
Polyclonal
Specificity:
Keratin 23 Antibody detects endogenous levels of total Keratin 23.
RRID:
AB_2842196
Cite Format: Affinity Biosciences Cat# DF9000, RRID:AB_2842196.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK 23; CK-23; CK23; Cytokeratin 23; Cytokeratin-23; Cytokeratin23; DKFZP434G032; HAIK 1; HAIK1; Histone deacetylase inducible keratin 23; Hyperacetylation inducible type I keratin; K1C23_HUMAN; K23; Keratin 23 (histone deacetylase inducible); Keratin 23; Keratin; Keratin type I cytoskeletal 23; Keratin-23; Keratin23; KRT 23; KRT23; MGC26158; type I cytoskeletal 23; Type I intermediate filament cytokeratin;

Immunogens

Immunogen:

A synthesized peptide derived from human Keratin 23, corresponding to a region within N-terminal amino acids.

Uniprot:

Q9C075(K1C23_HUMAN) >>Visit HPA database.

Gene(ID):
KRT23(25984)
Sequence:
MNSGHSFSQTPSASFHGAGGGWGRPRSFPRAPTVHGGAGGARISLSFTTRSCPPPGGSWGSGRSSPLLGGNGKATMQNLNDRLASYLEKVRALEEANMKLESRILKWHQQRDPGSKKDYSQYEENITHLQEQIVDGKMTNAQIILLIDNARMAVDDFNLKYENEHSFKKDLEIEVEGLRRTLDNLTIVTTDLEQEVEGMRKELILMKKHHEQEMEKHHVPSDFNVNVKVDTGPREDLIKVLEDMRQEYELIIKKKHRDLDTWYKEQSAAMSQEAASPATVQSRQGDIHELKRTFQALEIDLQTQYSTKSALENMLSETQSRYSCKLQDMQEIISHYEEELTQLRHELERQNNEYQVLLGIKTHLEKEITTYRRLLEGESEGTREESKSSMKVSATPKIKAITQETINGRLVLCQVNEIQKHA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
92
Bovine
83
Rabbit
83
Pig
80
Horse
73
Dog
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). KRT23 promotes proliferation invasion and metastasis of gastric cancer through epithelial-mesenchymal transition mediated by the PI3K/AKT/mTOR signaling pathway. Translational cancer research, 2025 (PubMed: 41245804) [IF=1.5]

Application: IHC    Species: human    Sample: GC cells

Figure 2 The expression of KRT23 in GC cells. (A) The expression of KRT23 was detected by immunohistochemistry staining method (magnification ×400). (B) RT-qPCR was used to detect the mRNA expression of KRT23 in different GC cells. (C,D) Western blot detected the expression of KRT23 protein in different GC cells. (E,F) RT-qPCR was used to detect the mRNA silencing level of KRT23 in MGC803 and SGC7901 cells. (G,H) Western blots detected the protein silencing level of KRT23 in MGC803 and SGC7901 cells. **, P

Application: WB    Species: human    Sample: GC cells

Figure 2 The expression of KRT23 in GC cells. (A) The expression of KRT23 was detected by immunohistochemistry staining method (magnification ×400). (B) RT-qPCR was used to detect the mRNA expression of KRT23 in different GC cells. (C,D) Western blot detected the expression of KRT23 protein in different GC cells. (E,F) RT-qPCR was used to detect the mRNA silencing level of KRT23 in MGC803 and SGC7901 cells. (G,H) Western blots detected the protein silencing level of KRT23 in MGC803 and SGC7901 cells. **, P

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