Product: MAGB4 Antibody
Catalog: DF9017
Description: Rabbit polyclonal antibody to MAGB4
Application: WB IF/ICC
Reactivity: Human, Monkey
Mol.Wt.: 39kD,35kD(Calculated).
Uniprot: O15481 | P43366 | O15479 | Q96LZ2 | Q96M61
RRID: AB_2842213

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Clonality:
Polyclonal
Specificity:
MAGB4 Antibody detects endogenous levels of total MAGB4.
RRID:
AB_2842213
Cite Format: Affinity Biosciences Cat# DF9017, RRID:AB_2842213.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cancer/testis antigen family 3, member 6; CT3.6; MAGB4_HUMAN; MAGE B4 antigen; MAGE-B4 antigen; MAGEB4; melanoma antigen family B, 4; Melanoma associated antigen B4; Melanoma-associated antigen B4; MGC33144; Cancer/testis antigen 3.1; CT3.1; DAM10; DSS AHC critical interval MAGE superfamily 10; DSS-AHC critical interval MAGE superfamily 10; MAGB1_HUMAN; MAGE B1 antigen; MAGE Xp; MAGE-B1 antigen; MAGE-XP antigen; MAGEB1; MAGEL1; MAGEXP antigen; melanoma antigen family B, 1; Melanoma associated antigen B1; Melanoma-associated antigen B1; MGC9322; Cancer/testis antigen 3.2; Cancer/testis antigen family 3, member 2; CT3.2; DAM6; DSS AHC critical interval MAGE superfamily 6; DSS/AHC critical interval gene, from MAGE superfamily, 6; MAGE B2 antigen; MAGE XP 2; MAGE XP 2 antigen; Mage-b2; Mage-rs2; melanoma antigen family B, 2; Melanoma antigen, family B, 1; Melanoma associated antigen B2; MGC2643; MGC26438; Smage-2 protein; Smage2; FLJ32965; MAGBA_HUMAN; MAGE B10 antigen; MAGE family testis and tumor specific protein; MAGE-B10 antigen; MAGEB10; melanoma antigen family B, 10; Melanoma associated antigen B10; Melanoma-associated antigen B10; MGC120394; OTTHUMP00000043236; B430216B18Rik; MAGBI_HUMAN; MAGE-B18 antigen; MAGEB18; MAGEB18 antigen; Melanoma antigen family B, 18; Melanoma-associated antigen B18; MGC114496; RGD1560369; RP23-289C5.3;

Immunogens

Immunogen:

A synthesized peptide derived from human MAGB4, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
O15481 MAGB4_HUMAN:

Expressed in testis.

P43366 MAGB1_HUMAN:

Expressed only in testis.

O15479 MAGB2_HUMAN:

Expressed in testis and placenta, and in a significant fraction of tumors of various histologic types.

Sequence:
MPRGQKSKLRAREKRQRTRGQTQDLKVGQPTAAEKEESPSSSSSVLRDTASSSLAFGIPQEPQREPPTTSAAAAMSCTGSDKGDESQDEENASSSQASTSTERSLKDSLTRKTKMLVQFLLYKYKMKEPTTKAEMLKIISKKYKEHFPEIFRKVSQRTELVFGLALKEVNPTTHSYILVSMLGPNDGNQSSAWTLPRNGLLMPLLSVIFLNGNCAREEEIWEFLNMLGIYDGKRHLIFGEPRKLITQDLVQEKYLEYQQVPNSDPPRYQFLWGPRAHAETSKMKVLEFLAKVNDTTPNNFPLLYEEALRDEEERAGARPRVAARRGTTAMTSAYSRATSSSSSQPM

MPRGQKSKLRAREKRRKAREETQGLKVAHATAAEKEECPSSSPVLGDTPTSSPAAGIPQKPQGAPPTTTAAAAVSCTESDEGAKCQGEENASFSQATTSTESSVKDPVAWEAGMLMHFILRKYKMREPIMKADMLKVVDEKYKDHFTEILNGASRRLELVFGLDLKEDNPSGHTYTLVSKLNLTNDGNLSNDWDFPRNGLLMPLLGVIFLKGNSATEEEIWKFMNVLGAYDGEEHLIYGEPRKFITQDLVQEKYLKYEQVPNSDPPRYQFLWGPRAYAETTKMKVLEFLAKMNGATPRDFPSHYEEALRDEEERAQVRSSVRARRRTTATTFRARSRAPFSRSSHPM

MPRGQKSKLRAREKRRKARDETRGLNVPQVTEAEEEEAPCCSSSVSGGAASSSPAAGIPQEPQRAPTTAAAAAAGVSSTKSKKGAKSHQGEKNASSSQASTSTKSPSEDPLTRKSGSLVQFLLYKYKIKKSVTKGEMLKIVGKRFREHFPEILKKASEGLSVVFGLELNKVNPNGHTYTFIDKVDLTDEESLLSSWDFPRRKLLMPLLGVIFLNGNSATEEEIWEFLNMLGVYDGEEHSVFGEPWKLITKDLVQEKYLEYKQVPSSDPPRFQFLWGPRAYAETSKMKVLEFLAKVNGTTPCAFPTHYEEALKDEEKAGV

MPRGQKSKLRAREKRRQARGGLEDLIDALDILEEEEESPPSASACLKDVFQSSLDGASNNPHGLREAQSTSTSATAASHTRHPEGVNDQMEERPICTQDLEATDSFPRGPVDEKVIILVHYLLYKYQMKEPITKADMLRNVTQMSKSQFPVILSRASEHLELIFGLDLKEVEPNKHIYVLVNKLDLGCDAKLSDETGVPKTGLLMTVLGIIFTNGNCVAEEEVWKVFNTMGLYDGIEHFMFGEPRKLLTKDLVKENYLEYQQVPNSDPPRYQFLWGPRAHAETSKMKVLEFLAKVNDTAPSEFSNWYTEALQDEEERARARVAAKARVSATAGARSKVKSSKSSQLQ

MPRGQKSKLRAREKRHQARCENQDLGATQATVAEGESPSPAYLLFGDRPQNLPAAETPSIPEALQGAPSTTNAIAPVSCSSNEGASSQDEKSLGSSREAEGWKEDPLNKKVVSLVHFLLQKYETKEPITKGDMIKFVIRKDKCHFNEILKRASEHMELALGVDLKEVDPIRHYYAFFSKLDLTYDETTSDEEKIPKTGLLMIALGVIFLNGNRAPEEAVWEIMNMMGVYADRKHFLYGDPRKVMTKDLVQLKYLEYQQVPNSDPPRYEFLWGPRAHAETSKMKVLEFVAKIHDTVPSAFPSCYEEALRDEEQRTQARAAARAHTAAMANARSRTTSSSFSHAK

Research Backgrounds

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in testis.

Tissue Specificity:

Expressed only in testis.

Function:

May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

Tissue Specificity:

Expressed in testis and placenta, and in a significant fraction of tumors of various histologic types.

Function:

May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

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