MAGF1 Antibody - #DF9020
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9020, RRID:AB_2842216.
Fold/Unfold
MAGE F1 antigen; MAGE-F1 antigen; MAGEF 1; MAGEF1; MAGF1_HUMAN; Melanoma antigen family F, 1; Melanoma associated antigen F1; Melanoma-associated antigen F1; MGC19617;
Immunogens
- Q9HAY2 MAGF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLQTPESRGLPVPQAEGEKDGGHDGETRAPTASQERPKEELGAGREEGAAEPALTRKGARALAAKALARRRAYRRLNRTVAELVQFLLVKDKKKSPITRSEMVKYVIGDLKILFPDIIARAAEHLRYVFGFELKQFDRKHHTYILINKLKPLEEEEEEDLGGDGPRLGLLMMILGLIYMRGNSAREAQVWEMLRRLGVQPSKYHFLFGYPKRLIMEDFVQQRYLSYRRVPHTNPPEYEFSWGPRSNLEISKMEVLGFVAKLHKKEPQHWPVQYREALADEADRARAKARAEASMRARASARAGIHLW
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9HAY2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K38 | Ubiquitination | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| Y273 | Phosphorylation | Uniprot |
Research Backgrounds
Enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin ligases. Proposed to act through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. MAGEF1-NSMCE1 ubiquitin ligase complex promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. May negatively regulate genome integrity by inhibiting homologous recombination-mediated double-strand break DNA repair.
Ubiquitous.
Interacts (via MAGE domain) with RING-type zinc finger-containing E3 ubiquitin-protein ligases LNX1, TRIM27 and NSMCE1; the interaction is direct.
Restrictive clause
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For Research Use Only.
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