CENPC1 Antibody - #DF9057

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Product: CENPC1 Antibody
Catalog: DF9057
Description: Rabbit polyclonal antibody to CENPC1
Application: WB IHC
Reactivity: Human
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 106 kDa; 107kD(Calculated).
Uniprot: Q03188
RRID: AB_2842253

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Horse(83%), Sheep(100%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CENPC1 Antibody detects endogenous levels of total CENPC1.
RRID:
AB_2842253
Cite Format: Affinity Biosciences Cat# DF9057, RRID:AB_2842253.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CENP-C 1; CENP-C; CENPC_HUMAN; CENPC1; Centromere autoantigen C; Centromere autoantigen C1; Centromere protein C 1; Centromere protein C; hcp-4; ICEN7; Interphase centromere complex protein 7; MIF2;

Immunogens

Immunogen:
Uniprot:

Q03188(CENPC_HUMAN) >>Visit HPA database.

Gene(ID):
CENPC(1060)
Sequence:
MAASGLDHLKNGYRRRFCRPSRARDINTEQGQNVLEILQDCFEEKSLANDFSTNSTKSVPNSTRKIKDTCIQSPSKECQKSHPKSVPVSSKKKEASLQFVVEPSEATNRSVQAHEVHQKILATDVSSKNTPDSKKISSRNINDHHSEADEEFYLSVGSPSVLLDAKTSVSQNVIPSSAQKRETYTFENSVNMLPSSTEVSVKTKKRLNFDDKVMLKKIEIDNKVSDEEDKTSEGQERKPSGSSQNRIRDSEYEIQRQAKKSFSTLFLETVKRKSESSPIVRHAATAPPHSCPPDDTKLIEDEFIIDESDQSFASRSWITIPRKAGSLKQRTISPAESTALLQGRKSREKHHNILPKTLANDKHSHKPHPVETSQPSDKTVLDTSYALIGETVNNYRSTKYEMYSKNAEKPSRSKRTIKQKQRRKFMAKPAEEQLDVGQSKDENIHTSHITQDEFQRNSDRNMEEHEEMGNDCVSKKQMPPVGSKKSSTRKDKEESKKKRFSSESKNKLVPEEVTSTVTKSRRISRRPSDWWVVKSEESPVYSNSSVRNELPMHHNSSRKSTKKTNQSSKNIRKKTIPLKRQKTATKGNQRVQKFLNAEGSGGIVGHDEISRCSLSEPLESDEADLAKKKNLDCSRSTRSSKNEDNIMTAQNVPLKPQTSGYTCNIPTESNLDSGEHKTSVLEESGPSRLNNNYLMSGKNDVDDEEVHGSSDDSKQSKVIPKNRIHHKLVLPSNTPNVRRTKRTRLKPLEYWRGERIDYQGRPSGGFVISGVLSPDTISSKRKAKENIGKVNKKSNKKRICLDNDERKTNLMVNLGIPLGDPLQPTRVKDPETREIILMDLVRPQDTYQFFVKHGELKVYKTLDTPFFSTGKLILGPQEEKGKQHVGQDILVFYVNFGDLLCTLHETPYILSTGDSFYVPSGNYYNIKNLRNEESVLLFTQIKR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
92
Horse
83
Xenopus
50
Bovine
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03188 As Substrate

Site PTM Type Enzyme
K10 Ubiquitination
K45 Ubiquitination
S46 Phosphorylation
S52 Phosphorylation
S55 Phosphorylation
S73 Phosphorylation
S75 Phosphorylation
K84 Ubiquitination
S96 Phosphorylation
S110 Phosphorylation
K119 Ubiquitination
S126 Phosphorylation
S127 Phosphorylation
K128 Ubiquitination
T130 Phosphorylation
S133 Phosphorylation
S146 Phosphorylation
S158 Phosphorylation
S160 Phosphorylation
T167 Phosphorylation
S170 Phosphorylation
S176 Phosphorylation
S177 Phosphorylation
K180 Ubiquitination
T183 Phosphorylation
Y184 Phosphorylation
T185 Phosphorylation
S189 Phosphorylation
S225 Phosphorylation
T231 Phosphorylation
S240 Phosphorylation
S243 Phosphorylation
S250 Phosphorylation
Y252 Phosphorylation
K260 Ubiquitination
S261 Phosphorylation
T264 Phosphorylation
K271 Ubiquitination
K273 Ubiquitination
S274 Phosphorylation
S277 Phosphorylation
S290 Phosphorylation
T296 Phosphorylation
S308 Phosphorylation
S311 Phosphorylation
S316 Phosphorylation
T319 Phosphorylation
T331 Phosphorylation
S333 Phosphorylation
K349 Ubiquitination
K356 Ubiquitination
T372 Phosphorylation
S376 Phosphorylation
S384 Phosphorylation
K399 Ubiquitination
K414 Methylation
S439 Phosphorylation
K440 Ubiquitination
S447 Phosphorylation
S483 Phosphorylation
K507 Ubiquitination
S515 Phosphorylation
T516 Phosphorylation
T518 Phosphorylation
S520 Phosphorylation
S524 Phosphorylation
S528 Phosphorylation
K534 Sumoylation
S535 Phosphorylation
S538 Phosphorylation
Y541 Phosphorylation
K579 Acetylation
K593 Ubiquitination
S600 Phosphorylation
S610 Phosphorylation
S613 Phosphorylation
S615 Phosphorylation
S620 Phosphorylation
K655 Ubiquitination
S673 Phosphorylation
S679 Phosphorylation
S684 Phosphorylation
S709 Phosphorylation
S710 Phosphorylation
S713 Phosphorylation
K721 Sumoylation
K727 Ubiquitination
S732 Phosphorylation
T734 Phosphorylation
K746 Sumoylation
K746 Ubiquitination
Y750 Phosphorylation
Y758 Phosphorylation
S763 Phosphorylation
S773 Phosphorylation
K807 Ubiquitination
T808 Phosphorylation
T825 Phosphorylation
K860 Ubiquitination
K871 Ubiquitination
K880 Ubiquitination

Research Backgrounds

Function:

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.

Subcellular Location:

Nucleus. Chromosome>Centromere>Kinetochore. Chromosome>Centromere.
Note: Localizes exclusively in the kinetochore domain of centromeres.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Oligomer. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Binds to DAXX. Interacts with DNMT3B. Interacts directly with CENPA. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1. Interacts with MEIKIN (By similarity).

Family&Domains:

The MIF2 homology domain II targets centromeres and binds the alpha satellite DNA in vivo. The MIF2 homology domain III can induce CENPC dimerization/oligomerization.

Belongs to the CENP-C/MIF2 family.

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