CENPH Antibody | Affinity Biosciences

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IF/ICC

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CENPH Antibody - Western blot analysis of extracts from 293t, using CENPH Antibody.

CENPH Antibody - DF9058 staining A549 cells by IF/ICC.

Product:	CENPH Antibody
Catalog:	DF9058
Description:	Rabbit polyclonal antibody to CENPH
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	28 kDa; 28kD(Calculated).
Uniprot:	Q9H3R5
RRID:	AB_2842254

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Bovine(89%), Sheep(89%), Rabbit(86%), Dog(83%)

Clonality:

Polyclonal

Specificity:

CENPH Antibody detects endogenous levels of total CENPH.

RRID:

AB_2842254
Cite Format: Affinity Biosciences Cat# DF9058, RRID:AB_2842254.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CENP H; CENP-H; CENPH; CENPH_HUMAN; Centromere protein H; ICEN35; Interphase centromere complex protein 35; Kinetochore protein CENP H; NNF1; NNF1, MIND kinetochore complex component, homolog; PMF1;

Immunogens

Immunogen:

Uniprot:

Q9H3R5(CENPH_HUMAN) >>Visit HPA database.

Gene(ID):

CENPH(64946)

Sequence:

Q9H3R5 CENPH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEEQPQMQDADEPADSGGEGRAGGPPQVAGAQAACSEDRMTLLLRLRAQTKQQLLEYKSMVDASEEKTPEQIMQEKQIEAKIEDLENEIEEVKVAFEIKKLALDRMRLSTALKKNLEKISRQSSVLMDNMKHLLELNKLIMKSQQESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKIDLDSMENSERIKIIRQNLQMEIKITTVIQHVFQNLILGSKVNWAEDPALKEIVLQLEKNVDMM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Rabbit

Dog

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H3R5 As Substrate

Q9H3R5

Site	PTM Type	Source
M1	Acetylation	Uniprot
S16	Phosphorylation	Uniprot
R21	Methylation	Uniprot
K51	Ubiquitination	Uniprot
K58	Ubiquitination	Uniprot
S64	Phosphorylation	Uniprot
T68	Phosphorylation	Uniprot
K76	Ubiquitination	Uniprot
K81	Ubiquitination	Uniprot
K118	Ubiquitination	Uniprot
S124	Phosphorylation	Uniprot
K131	Ubiquitination	Uniprot
K138	Ubiquitination	Uniprot
K142	Ubiquitination	Uniprot
K153	Ubiquitination	Uniprot
K165	Ubiquitination	Uniprot
K171	Ubiquitination	Uniprot
K179	Ubiquitination	Uniprot
K183	Ubiquitination	Uniprot
S223	Phosphorylation	Uniprot
K224	Ubiquitination	Uniprot
K234	Ubiquitination	Uniprot

Research Backgrounds

Q9H3R5_CENPH_HUMAN

Function:

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.

Subcellular Location:

Nucleus. Chromosome>Centromere>Kinetochore.
Note: Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC during both interphase and metaphase.

Subunit Structure:

Self-associates. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with CENPK. Interacts with KIF2C and NDC80. Interacts with TRIM36 (By similarity).

Family&Domains:

Belongs to the CENP-H/MCM16 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CENPH Antibody - #DF9058