CENPK Antibody - #DF9059
| Product: | CENPK Antibody |
| Catalog: | DF9059 |
| Description: | Rabbit polyclonal antibody to CENPK |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Sheep, Rabbit |
| Mol.Wt.: | 32 kDa; 32kD(Calculated). |
| Uniprot: | Q9BS16 |
| RRID: | AB_2842255 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9059, RRID:AB_2842255.
Fold/Unfold
AF5 alpha; AF5alpha; B130045K24Rik; BB232189; C530004N04Rik; CENP K; CENPK; Centromere protein K; Centromeric protein K; FKSG 14; FKSG14; ICEN 37; ICEN37; Interphase centromere complex protein 37; Leucine zipper protein FKSG14; OTTHUMP00000161888; P33; Protein AF 5alpha; Protein AF5alpha; Solt; Solzt; SoxLZ/Sox6 binding protein Solt; SoxLZ/Sox6 leucine zipper binding protein;
Immunogens
Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.
- Q9BS16 CENPK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNQEDLDPDSTTDVGDVTNTEEELIRECEEMWKDMEECQNKLSLIGTETLTDSNAQLSLLIMQVKCLTAELSQWQKKTPETIPLTEDVLITLGKEEFQKLRQDLEMVLSTKESKNEKLKEDLEREQRWLDEQQQIMESLNVLHSELKNKVETFSESRIFNELKTKMLNIKEYKEKLLSTLGEFLEDHFPLPDRSVKKKKKNIQESSVNLITLHEMLEILINRLFDVPHDPYVKISDSFWPPYVELLLRNGIALRHPEDPTRIRLEAFHQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BS16 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K33 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot |
Research Backgrounds
Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
Nucleus. Chromosome>Centromere. Chromosome>Centromere>Kinetochore.
Note: Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.
Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.
Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Interacts directly with CENPH.
Belongs to the CENP-K/MCM22 family.
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