CENPQ Antibody | Affinity Biosciences

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CENPQ Antibody - Western blot analysis of CENPQ using Jurkat whole cell lysates.

Product:	CENPQ Antibody
Catalog:	DF9061
Description:	Rabbit polyclonal antibody to CENPQ
Application:	WB
Reactivity:	Human
Mol.Wt.:	31 kDa; 31kD(Calculated).
Uniprot:	Q7L2Z9
RRID:	AB_2842257

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

CENPQ Antibody detects endogenous levels of total CENPQ.

RRID:

AB_2842257
Cite Format: Affinity Biosciences Cat# DF9061, RRID:AB_2842257.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CENP Q; CENP-Q; Cenpq; CENPQ_HUMAN; Centromere protein Q; FLJ10545; OTTHUMP00000016579;

Immunogens

Immunogen:

Uniprot:

Q7L2Z9(CENPQ_HUMAN) >>Visit HPA database.

Gene(ID):

CENPQ(55166)

Sequence:

Q7L2Z9 CENPQ_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGKANASKKNAQQLKRNPKRKKDNEEVVLSENKVRNTVKKNKNHLKDLSSEGQTKHTNLKHGKTAASKRKTWQPLSKSTRDHLQTMMESVIMTILSNSIKEKEEIQYHLNFLKKRLLQQCETLKVPPKKMEDLTNVSSLLNMERARDKANEEGLALLQEEIDKMVETTELMTGNIQSLKNKIQILASEVEEEEERVKQMHQINSSGVLSLPELSQKTLKAPTLQKEILALIPNQNALLKDLDILHNSSQMKSMSTFIEEAYKKLDAS

PTMs - Q7L2Z9 As Substrate

Q7L2Z9

Site	PTM Type	Enzyme	Source
K10	Acetylation		Uniprot
S31	Phosphorylation		Uniprot
K34	Ubiquitination		Uniprot
K47	Ubiquitination		Uniprot
S50	Phosphorylation		Uniprot
K56	Ubiquitination		Uniprot
K71	Ubiquitination		Uniprot
K78	Ubiquitination		Uniprot
Y108	Phosphorylation		Uniprot
T123	Phosphorylation	P53350 (PLK1)	Uniprot
K130	Ubiquitination		Uniprot
T135	Phosphorylation	P53350 (PLK1)	Uniprot
S138	Phosphorylation	P53350 (PLK1)	Uniprot
S139	Phosphorylation	P53350 (PLK1)	Uniprot
K149	Ubiquitination		Uniprot
K180	Ubiquitination		Uniprot
K182	Ubiquitination		Uniprot
K217	Ubiquitination		Uniprot
S248	Phosphorylation	P53350 (PLK1)	Uniprot
S249	Phosphorylation	P53350 (PLK1)	Uniprot
S253	Phosphorylation	P53350 (PLK1)	Uniprot
S255	Phosphorylation	P53350 (PLK1)	Uniprot
T256	Phosphorylation	P53350 (PLK1)	Uniprot
S268	Phosphorylation		Uniprot

Research Backgrounds

Q7L2Z9_CENPQ_HUMAN

Function:

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Plays an important role in chromosome congression and in the recruitment of CENP-O complex (which comprises CENPO, CENPP, CENPQ and CENPU), CENPE and PLK1 to the kinetochores.

PTMs:

Phosphorylation at Ser-50 is essential for CENPE recruitment to kinetochores and orderly chromosome congression.

Subcellular Location:

Nucleus. Chromosome>Centromere.
Note: Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.

Subunit Structure:

Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU.

Family&Domains:

Belongs to the CENP-Q/OKP1 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CENPQ Antibody - #DF9061