NKIRAS1 Antibody - #DF9067
| Product: | NKIRAS1 Antibody |
| Catalog: | DF9067 |
| Description: | Rabbit polyclonal antibody to NKIRAS1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 22 kDa; 22kD(Calculated). |
| Uniprot: | Q9NYS0 |
| RRID: | AB_2842263 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9067, RRID:AB_2842263.
Fold/Unfold
2400004O09Rik; I kappa B interacting Ras like protein 1; I-kappa-B-interacting Ras-like protein 1; Kappa B Ras 1; Kappa B Ras protein 1; Kappa B-Ras protein 1; KappaB Ras1; KappaB-Ras1; KBRAS 1; KBRAS1; KBRS1_HUMAN; NF kappa B inhibitor interacting Ras like protein 1; NF-kappa-B inhibitor-interacting Ras-like protein 1; NFKB inhibitor interacting Ras like 1; NFKB inhibitor interacting Ras like protein 1; NKIRAS 1; nkiras1;
Immunogens
- Q9NYS0 KBRS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKGCKVVVCGLLSVGKTAILEQLLYGNHTIGMEDCETMEDVYMASVETDRGVKEQLHLYDTRGLQEGVELPKHYFSFADGFVLVYSVNNLESFQRVELLKKEIDKFKDKKEVAIVVLGNKIDLSEQRQVDAEVAQQWAKSEKVRLWEVTVTDRKTLIEPFTLLASKLSQPQSKSSFPLPGRKNKGNSNSEN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NYS0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K54 | Ubiquitination | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| K143 | Acetylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| T152 | Phosphorylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot |
Research Backgrounds
Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.
Cytoplasm.
Widely expressed.
Interacts with both NF-kappa-B inhibitor alpha (NFKBIA) and beta (NFKBIB) in vitro. However, it probably only interacts with NFKBIB in vivo. Forms a complex with NFKBIB and NF-kappa-B heterodimer (p50/NFKB1 and p65/RELA). Also interacts with c-Rel (REL).
In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Leu residues, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.
Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.
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