NKAP Antibody | Affinity Biosciences

Product:	NKAP Antibody
Catalog:	DF9069
Description:	Rabbit polyclonal antibody to NKAP
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Rabbit
Mol.Wt.:	47 kDa; 47kD(Calculated).
Uniprot:	Q8N5F7
RRID:	AB_2842265

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Rabbit(100%)

Clonality:

Polyclonal

Specificity:

NKAP Antibody detects endogenous levels of total NKAP.

RRID:

AB_2842265
Cite Format: Affinity Biosciences Cat# DF9069, RRID:AB_2842265.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

NF-kappa-B-activating protein; NFKB activating protein; Nkap; NKAP_HUMAN; Nuclear NF kappaB activating protein;

Immunogens

Immunogen:

Uniprot:

Q8N5F7(NKAP_HUMAN) >>Visit HPA database.

Gene(ID):

NKAP(79576)

Sequence:

Q8N5F7 NKAP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPVSGSRSPDREASGSGGRRRSSSKSPKPSKSARSPRGRRSRSHSCSRSGDRNGLTHQLGGLSQGSRNQSYRSRSRSRSRERPSAPRGIPFASASSSVYYGSYSRPYGSDKPWPSLLDKEREESLRQKRLSERERIGELGAPEVWGLSPKNPEPDSDEHTPVEDEEPKKSTTSASTSEEEKKKKSSRSKERSKKRRKKKSSKRKHKKYSEDSDSDSDSETDSSDEDNKRRAKKAKKKEKKKKHRSKKYKKKRSKKSRKESSDSSSKESQEEFLENPWKDRTKAEEPSDLIGPEAPKTLTSQDDKPLNYGHALLPGEGAAMAEYVKAGKRIPRRGEIGLTSEEIASFECSGYVMSGSRHRRMEAVRLRKENQIYSADEKRALASFNQEERRKRENKILASFREMVYRKTKGKDDK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N5F7 As Substrate

Q8N5F7

Site	PTM Type	Source
S5	Phosphorylation	Uniprot
S7	Phosphorylation	Uniprot
S9	Phosphorylation	Uniprot
S15	Phosphorylation	Uniprot
S17	Phosphorylation	Uniprot
S33	Phosphorylation	Uniprot
S46	Phosphorylation	Uniprot
S50	Phosphorylation	Uniprot
S64	Phosphorylation	Uniprot
S67	Phosphorylation	Uniprot
S80	Phosphorylation	Uniprot
S85	Phosphorylation	Uniprot
Y104	Phosphorylation	Uniprot
K112	Acetylation	Uniprot
S149	Phosphorylation	Uniprot
K151	Ubiquitination	Uniprot
S157	Phosphorylation	Uniprot
T161	Phosphorylation	Uniprot
S171	Phosphorylation	Uniprot
T172	Phosphorylation	Uniprot
S178	Phosphorylation	Uniprot
Y209	Phosphorylation	Uniprot
S210	Phosphorylation	Uniprot
S213	Phosphorylation	Uniprot
S224	Phosphorylation	Uniprot
Y249	Phosphorylation	Uniprot
S261	Phosphorylation	Uniprot
S262	Phosphorylation	Uniprot
S264	Phosphorylation	Uniprot
S265	Phosphorylation	Uniprot
S266	Phosphorylation	Uniprot
S269	Phosphorylation	Uniprot
K283	Sumoylation	Uniprot
K283	Ubiquitination	Uniprot
K305	Ubiquitination	Uniprot
Y324	Phosphorylation	Uniprot
K326	Ubiquitination	Uniprot
R334	Methylation	Uniprot
S355	Phosphorylation	Uniprot
S357	Phosphorylation	Uniprot
Y374	Phosphorylation	Uniprot
S375	Phosphorylation	Uniprot
K379	Ubiquitination	Uniprot

Research Backgrounds

Q8N5F7_NKAP_HUMAN

Function:

Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T-cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the Notch corepressor complex. Interacts with CIR1 and HDAC3.

Family&Domains:

Belongs to the NKAP family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

NKAP Antibody - #DF9069