GOLT1B Antibody - #DF9071

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Product: GOLT1B Antibody
Catalog: DF9071
Description: Rabbit polyclonal antibody to GOLT1B
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 15 kDa; 15kD(Calculated).
Uniprot: Q9Y3E0
RRID: AB_2842267

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GOLT1B Antibody detects endogenous levels of total GOLT1B.
RRID:
AB_2842267
Cite Format: Affinity Biosciences Cat# DF9071, RRID:AB_2842267.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CGI-141; GCT2; Germ cell tumor 2; Golgi transport 1 homolog B; Golgi transport 1B; GOLT1B; GOT1; GOT1B_HUMAN; hGOT1a; Putative NF-kappa-B-activating protein 470; Vesicle transport protein GOT1B; YMR292W;

Immunogens

Immunogen:
Uniprot:

Q9Y3E0(GOT1B_HUMAN) >>Visit HPA database.

Gene(ID):
GOLT1B(51026)
Expression:
Q9Y3E0 GOT1B_HUMAN:

Widely expressed. Tends to be up-regulated in seminomas compared to normal testis.

Sequence:
MISLTDTQKIGMGLTGFGVFFLFFGMILFFDKALLAIGNVLFVAGLAFVIGLERTFRFFFQKHKMKATGFFLGGVFVVLIGWPLIGMIFEIYGFFLLFRGFFPVVVGFIRRVPVLGSLLNLPGIRSFVDKVGESNNMV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y3E0 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K62 Ubiquitination
K130 Ubiquitination

Research Backgrounds

Function:

May be involved in fusion of ER-derived transport vesicles with the Golgi complex.

Subcellular Location:

Golgi apparatus membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Tends to be up-regulated in seminomas compared to normal testis.

Family&Domains:

Belongs to the GOT1 family.

References

1). Vesicle transporter GOLT1B mediates the cell membrane localization of DVL2 and PD-L2 and promotes colorectal cancer metastasis. Cancer Cell International, 2021 (PubMed: 34059062) [IF=5.8]

Application: WB    Species: Mice    Sample: HCT116 and RKO cells

Fig. 2 GOLT1B promotes CRC cell proliferation, invasion and migration. a, b GOLT1B mRNA and protein level were determined by qPCR and western blot in HCT116 and RKO cells. c, d Cell migration were determined by wound healing experiment after siRNA transfection or overexpression of GOLT1B, respectively. e, f Cell invasion were detected by transwell assays with matrigel after siRNA transfection or overexpression of GOLT1B, respectively. g Effect of GOLT1B on proliferation of colorectal cancer cells in vitro. All data are shown as mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Application: IHC    Species: Mice    Sample: HCT116 cells

Fig. 3 GOLT1B promotes metastasis of colorectal cancer in vivo. a GOLT1B stable overexpression was verified by qPCR and western blot. b The migration and invasion ability of HCT116 cells stablely transfected with control vector or GOLT1B were determined by transwell assays in vitro. c The average bioluminescence imaging signals in mice bearing liver metastatic burden was determined by IVIS imaging (HCT116Vector vs. HCT116GOLT1B, n = 8). d Body weight curves of mice in two groups. e Representative images of metastatic foci (pointed out with red arrow) in mice liver. f The number of liver metastatic foci in control vector and GOLT1B group. g IHC staining of GOLT1B and H&E staining in liver metastatic loci

Application: IF/ICC    Species: Mice    Sample: HCT116 cells

Fig. 5 GOLT1B interacts with DVL2 to promote it transport and localization to the cell membrane. a The nucleus and cytoplasm separation experiment showed that GOLT1B overexpression increased the level of nuclear β-catenin. b The cell membrane and cytoplasm separation experiment showed that GOLT1B could induce DVL2 upregualtion on the cell membrane. c CO-IP experiment confirmed the interaction between GOLT1B and DVL2. d Immunofluorescence experiment showed that GOLT1B and DVL2 were co-localized

Application: WB    Species: human    Sample: HCT116 and RKO cells

Fig. 2 |GOLT1B promotes CRC cell proliferation, invasion and migration. a, b GOLT1B mRNA and protein level were determined by qPCR and western blot in HCT116 and RKO cells.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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