SP6 Antibody - #DF9082
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| Product: | SP6 Antibody |
| Catalog: | DF9082 |
| Description: | Rabbit polyclonal antibody to SP6 |
| Application: | WB IHC |
| Cited expt.: | |
| Reactivity: | Human, Mouse, Monkey |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog |
| Mol.Wt.: | 40 kDa; 40kD(Calculated). |
| Uniprot: | Q3SY56 |
| RRID: | AB_2842278 |
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SP6 Antibody detects endogenous levels of total SP6.
RRID:
AB_2842278
Cite Format: Affinity Biosciences Cat# DF9082, RRID:AB_2842278.
Cite Format: Affinity Biosciences Cat# DF9082, RRID:AB_2842278.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
MGC119665; Epfn; EPIPROFIN; KLF14; Krueppel-like factor 14; MGC119662; MGC119663; MGC119664; Sp6; Sp6 transcription factor; SP6_HUMAN; Transcription factor Sp6;
Immunogens
Immunogen:
A synthesized peptide derived from human SP6, corresponding to a region within the internal amino acids.
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q3SY56 SP6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLTAVCGSLGSQHTEAPHASPPRLDLQPLQTYQGHTSPEAGDYPSPLQPGELQSLPLGPEVDFSQGYELPGASSRVTCEDLESDSPLAPGPFSKLLQPDMSHHYESWFRPTHPGAEDGSWWDLHPGTSWMDLPHTQGALTSPGHPGALQAGLGGYVGDHQLCAPPPHPHAHHLLPAAGGQHLLGPPDGAKALEVAAPESQGLDSSLDGAARPKGSRRSVPRSSGQTVCRCPNCLEAERLGAPCGPDGGKKKHLHNCHIPGCGKAYAKTSHLKAHLRWHSGDRPFVCNWLFCGKRFTRSDELQRHLQTHTGTKKFPCAVCSRVFMRSDHLAKHMKTHEGAKEEAAGAASGEGKAGGAVEPPGGKGKREAEGSVAPSN
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Zebrafish
80
Sheep
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
Function:
Promotes cell proliferation.
Subcellular Location:
Nucleus.
Tissue Specificity:
Ubiquitous.
Family&Domains:
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the Sp1 C2H2-type zinc-finger protein family.
References
1). FOXO4-SP6 axis controls surface epithelium commitment by mediating epigenomic remodeling. Stem cell reports, 2025
(PubMed: 40086444)
[IF=5.9]
Application: IF/ICC Species: human Sample:
Figure 4 SP6 depletion impairs SE commitment (A) Genome browser view of FOXO4, H3K4me3, and RNA-seq signal across SP6 loci. (B) qRT-PCR analysis for expression level of SP6 in scrambled shRNA- and shFOXO4-treated cells on D3 and D7. qRT-PCR values were normalized to the values in scrambled shRNA group. Values are shown as means ± SD (n = 3 independent experiments; ∗∗∗p < 0.001 t test). (C) qRT-PCR analysis for expression level of FOXO4 in scrambled shRNA- and shSP6-treated differentiated cells on D3. qRT-PCR values were normalized to the values in scrambled shRNA group. Values are shown as means ± SD (n = 3 independent experiments; ns means not statistically significant, t test). (D) Immunostaining for SP6 in hESCs and differentiated cells on D3. Scale bar, 100 μm. (E) Phase-contrast images and immunostaining for KRT18 and TP63 in scrambled shRNA- and shSP6-treated differentiated cells on D7. Scale bar, 100 μm. (F) qRT-PCR analysis for expression levels of representative genes of SE hallmark and regulators in scrambled shRNA- and shSP6-treated differentiated cells on D3. qRT-PCR values were normalized to the values in scrambled shRNA group. Values are shown as means ± SD (n = 3 independent experiments; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 t test). (G) GSEA for SE identity genes in the gene expression matrix of shSP6- versus scrambled shRNA-treated differentiated cells on D3 and D7. The used SE identity gene set is from published research (Huang et al., 2022). (H) GO (biological process) analysis of the up-regulated (left) and down-regulated genes (right) upon SP6 knockdown. (I) GSEA for down-regulated genes upon SP6 knockdown in the gene expression matrix of shFOXO4- versus scrambled shRNA-treated differentiated cells on D3 and D7.
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