Product: NIP7 Antibody
Catalog: DF9138
Description: Rabbit polyclonal antibody to NIP7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 20 kDa; 20kD(Calculated).
Uniprot: Q9Y221
RRID: AB_2842334

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
NIP7 Antibody detects endogenous levels of total NIP7.
RRID:
AB_2842334
Cite Format: Affinity Biosciences Cat# DF9138, RRID:AB_2842334.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosome subunit biogenesis protein NIP7 homolog; CGI 37; CGI37; FLJ10296; HSPC031; HSPC180; KD93; NIP 7; NIP7; NIP7 nucleolar pre rRNA processing protein; NIP7_HUMAN; Nuclear import 7; Nuclear import 7 homolog (S. cerevisiae); Nuclear import 7 homolog; OK/SW cl.76; OK/SW cl.78; OTTHUMP00000174889;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y221 NIP7_HUMAN:

Expressed in hematopoietic stem/progenitor cells.

Sequence:
MRPLTEEETRVMFEKIAKYIGENLQLLVDRPDGTYCFRLHNDRVYYVSEKIMKLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKYKVWIKPGAEQSFLYGNHVLKSGLGRITENTSQYQGVVVYSMADIPLGFGVAAKSTQDCRKVDPMAIVVFHQADIGEYVRHEETLT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
89
Xenopus
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y221 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
T9 Phosphorylation
K15 Ubiquitination
R30 Methylation
R43 Methylation
K50 Ubiquitination
K53 Ubiquitination
K62 Ubiquitination
K72 Acetylation
K72 Ubiquitination
Y88 Phosphorylation
K94 Ubiquitination
K96 Ubiquitination
K100 Ubiquitination
K115 Ubiquitination
K148 Ubiquitination
K155 Ubiquitination

Research Backgrounds

Function:

Required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in hematopoietic stem/progenitor cells.

Subunit Structure:

Monomer. Interacts with pre-ribosome complex. May bind to RNA (By similarity). Interacts with NOL8. May interact with SBDS. Interacts with FTSJ3.

Family&Domains:

Belongs to the NIP7 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.