ADAM28 Antibody - #DF9163

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Product: ADAM28 Antibody
Catalog: DF9163
Description: Rabbit polyclonal antibody to ADAM28
Application: WB
Reactivity: Human, Rat, Monkey
Prediction: Pig, Rabbit, Dog
Mol.Wt.: 87kDa; 87kD(Calculated).
Uniprot: Q9UKQ2
RRID: AB_2842359

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat,Monkey
Prediction:
Pig(80%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ADAM28 Antibody detects endogenous levels of total ADAM28.
RRID:
AB_2842359
Cite Format: Affinity Biosciences Cat# DF9163, RRID:AB_2842359.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A disintegrin and metalloproteinase domain 28; ADAM 28; ADAM metallopeptidase domain 28; ADAM23; Disintegrin and metalloproteinase domain containing protein 28; eMDC II; eMDCII; epididymal Metalloproteinase like disintegrin like and cysteine rich protein II; MDC L; MDCL; Metalloproteinase like disintegrin like and cysteine rich protein L;

Immunogens

Immunogen:
Uniprot:

Q9UKQ2(ADA28_HUMAN) >>Visit HPA database.

Gene(ID):
ADAM28(10863)
Expression:
Q9UKQ2 ADA28_HUMAN:

Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.

Sequence:
MLQGLLPVSLLLSVAVSAIKELPGVKKYEVVYPIRLHPLHKREAKEPEQQEQFETELKYKMTINGKIAVLYLKKNKNLLAPGYTETYYNSTGKEITTSPQIMDDCYYQGHILNEKVSDASISTCRGLRGYFSQGDQRYFIEPLSPIHRDGQEHALFKYNPDEKNYDSTCGMDGVLWAHDLQQNIALPATKLVKLKDRKVQEHEKYIEYYLVLDNGEFKRYNENQDEIRKRVFEMANYVNMLYKKLNTHVALVGMEIWTDKDKIKITPNASFTLENFSKWRGSVLSRRKRHDIAQLITATELAGTTVGLAFMSTMCSPYSVGVVQDHSDNLLRVAGTMAHEMGHNFGMFHDDYSCKCPSTICVMDKALSFYIPTDFSSCSRLSYDKFFEDKLSNCLFNAPLPTDIISTPICGNQLVEMGEDCDCGTSEECTNICCDAKTCKIKATFQCALGECCEKCQFKKAGMVCRPAKDECDLPEMCNGKSGNCPDDRFQVNGFPCHHGKGHCLMGTCPTLQEQCTELWGPGTEVADKSCYNRNEGGSKYGYCRRVDDTLIPCKANDTMCGKLFCQGGSDNLPWKGRIVTFLTCKTFDPEDTSQEIGMVANGTKCGDNKVCINAECVDIEKAYKSTNCSSKCKGHAVCDHELQCQCEEGWIPPDCDDSSVVFHFSIVVGVLFPMAVIFVVVAMVIRHQSSREKQKKDQRPLSTTGTRPHKQKRKPQMVKAVQPQEMSQMKPHVYDLPVEGNEPPASFHKDTNALPPTVFKDNPVSTPKDSNPKA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
80
Bovine
56
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKQ2 As Substrate

Site PTM Type Enzyme
Y28 Phosphorylation
Y237 Phosphorylation
S282 Phosphorylation

Research Backgrounds

Function:

May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation.

PTMs:

Pro-domain removal and maturation may be, at least in part, autocatalytic.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.

Family&Domains:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

References

1). ADAM28 from both endothelium and gastric cancer cleaves von Willebrand Factor to eliminate von Willebrand Factor-induced apoptosis of gastric cancer cells. European Journal of Pharmacology, 2021 (PubMed: 33675784) [IF=4.2]

Application: WB    Species: Human    Sample: gastric epithelial cells and gastric cancer cell

Fig. 1. The expression of ADAM28 is related to the prognosis of gastric cancer patients. (A) According to data from the TCGA database, Kaplan-Meier analyzed the relationship between overall survival and ADAM28 expression in gastric cancer patients. The overall survival rate was calculated by log-rank test and compared among different groups. (B) According to data from the TCGA database, differential ADAM28 mRNA levels (log2 intensity) in DGAD, GAAD, GITAD, GPAD, GTAD, MGAD, SIAD and normal people. DGAD, Diffuse Gastric Adenocarcinoma; GAAD, Gastric Adenocarcinoma; GITAD, Gastric Intestinal Type Adenocarcinoma; GPAD, Gastric Papillary Adenocarcinoma; GTAD, Gastric Tubular Adenocarcinoma; MGAD, Mucinous Gastric Adenocarcinoma; SIAD, Signet Ring Cell Gastric Adenocarcinoma. (C) ADAM28 protein expression levels in GES-1 normal gastric epithelial cells and five gastric cancer cell lines. (D) QRT-PCR analysis of the relative mRNA expression levels of ADAM28 in GES-1 normal gastric epithelial cells and five gastric cancer cell lines (*P < 0.05; **P < 0.01). (E) Co-expression correlation analysis of ADAM28 and vWF in gastric cancer based on TCGA database. Each bar represented the mean ± S.D. of three independent experiments.
2). Hypoxia promotes the expression of Von Willebrand factor in breast cancer cells by up-regulating the transcription factor YY1 and down-regulating the hsa-miR-424. European Journal of Pharmacology, 2022 (PubMed: 36202224) [IF=4.2]

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