ADAM33 Antibody - #DF9166

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Product: ADAM33 Antibody
Catalog: DF9166
Description: Rabbit polyclonal antibody to ADAM33
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 88 kDa; 88kD(Calculated).
Uniprot: Q9BZ11
RRID: AB_2842362

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
ADAM33 Antibody detects endogenous levels of total ADAM33.
RRID:
AB_2842362
Cite Format: Affinity Biosciences Cat# DF9166, RRID:AB_2842362.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A disintegrin and metalloprotease 33; A disintegrin and metalloproteinase domain 33; ADA33_HUMAN; ADAM 33; ADAM metallopeptidase domain 33; ADAM33; C20orf153; Disintegrin and metalloproteinase domain-containing protein 33; Disintegrin and reprolysin metalloproteinase family protein; DJ964F7.1; DKFZp434K0521; FLJ35308; FLJ36751; Metalloprotease disintegrin; MGC149823; MGC71889; PRO1891; UNQ873;

Immunogens

Immunogen:
Uniprot:

Q9BZ11(ADA33_HUMAN) >>Visit HPA database.

Gene(ID):
ADAM33(80332)
Expression:
Q9BZ11 ADA33_HUMAN:

Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.

Sequence:
MGWRPRRARGTPLLLLLLLLLLWPVPGAGVLQGHIPGQPVTPHWVLDGQPWRTVSLEEPVSKPDMGLVALEAEGQELLLELEKNHRLLAPGYIETHYGPDGQPVVLAPNHTDHCHYQGRVRGFPDSWVVLCTCSGMSGLITLSRNASYYLRPWPPRGSKDFSTHEIFRMEQLLTWKGTCGHRDPGNKAGMTSLPGGPQSRGRREARRTRKYLELYIVADHTLFLTRHRNLNHTKQRLLEVANYVDQLLRTLDIQVALTGLEVWTERDRSRVTQDANATLWAFLQWRRGLWAQRPHDSAQLLTGRAFQGATVGLAPVEGMCRAESSGGVSTDHSELPIGAAATMAHEIGHSLGLSHDPDGCCVEAAAESGGCVMAAATGHPFPRVFSACSRRQLRAFFRKGGGACLSNAPDPGLPVPPALCGNGFVEAGEECDCGPGQECRDLCCFAHNCSLRPGAQCAHGDCCVRCLLKPAGALCRQAMGDCDLPEFCTGTSSHCPPDVYLLDGSPCARGSGYCWDGACPTLEQQCQQLWGPGSHPAPEACFQVVNSAGDAHGNCGQDSEGHFLPCAGRDALCGKLQCQGGKPSLLAPHMVPVDSTVHLDGQEVTCRGALALPSAQLDLLGLGLVEPGTQCGPRMVCQSRRCRKNAFQELQRCLTACHSHGVCNSNHNCHCAPGWAPPFCDKPGFGGSMDSGPVQAENHDTFLLAMLLSVLLPLLPGAGLAWCCYRLPGAHLQRCSWGCRRDPACSGPKDGPHRDHPLGGVHPMELGPTATGQPWPLDPENSHEPSSHPEKPLPAVSPDPQADQVQMPRSCLW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
91
Sheep
91
Rabbit
91
Xenopus
82
Chicken
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BZ11 As Substrate

Site PTM Type Enzyme
K187 Methylation
T489 Phosphorylation
S746 Phosphorylation

Research Backgrounds

PTMs:

The precursor is cleaved by a furin endopeptidase.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.

Family&Domains:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

References

1). Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling. Molecular Medicine Reports, 2021 (PubMed: 33982767) [IF=3.4]

Application: IHC    Species: Human    Sample: lung tissue

Figure 2. Immunohistochemical staining of ADAM33 in lung tissue of a patient with asthma. (A) Representative images showed that ADAM33 was mainly expressed in epithelial cells, smooth muscle cells, endothelial cells and interstitial inflammatory cells in the lung tissue of asthmatic patients. Scale bar, 200 µm. (B) Representative images showed that ADAM33 was also expressed in airway VSMCs in the lung tissue of asthmatic patients. Arrows indicate ADAM33 stained in brown in airway VSMCs in the lung tissue of asthmatic patients. Scale bar, 100 µm. ADAM33, a disintegrin and metalloproteinase-33; VSMCs, vascular smooth muscle cells.

Application: WB    Species: Human    Sample: HASMCs

Figure 4. Validation of lentivirus silencing effect of ADAM33 in HASMCs. (A) Comparison of ADAM33 mRNA levels among HASMCs in groups of blank control (no treatment), LV-NC and ADAM33 silencing with LV-ADAM33-shRNA 1–3 with reverse transcription-quantitative PCR. (B) Comparison of ADAM33 protein levels among HASMCs in groups of blank control (no treatment), LV-NC and ADAM33 silencing with LV-ADAM33-shRNA with western blotting. (C) Semi-quantitative analysis for the ADAM33 protein levels among HASMCs in blank control, LV-NC and LV-ADAM33-shRNA groups. *P<0.05. ADAM33, a disintegrin and metalloproteinase-33; HASMCs, human aortic smooth muscle cells; LV, lentiviral vector; NC, negative control; shRNA, short-hairpin RNA.

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