ADAMTS16 Antibody | Affinity Biosciences

WB
IHC
Cites

1/4

ADAMTS16 Antibody - Western blot analysis of ADAMTS16 using HuvEc whole cell lysates.

ADAMTS16 Antibody - DF9173 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

ADAMTS16 Antibody - DF9173 at 1/100 staining Mouse kidney tissue by IHC-P.

ADAMTS16 Antibody - Figure 2.

Product:	ADAMTS16 Antibody
Catalog:	DF9173
Description:	Rabbit polyclonal antibody to ADAMTS16
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	136kDa; 136kD(Calculated).
Uniprot:	Q8TE57
RRID:	AB_2842369

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ADAMTS16 Antibody detects endogenous levels of total ADAMTS16.

RRID:

AB_2842369
Cite Format: Affinity Biosciences Cat# DF9173, RRID:AB_2842369.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A disintegrin and metalloproteinase with thrombospondin motifs 16; ADAMTS 16; ADAMTS16; EC 3.4.24.;

Immunogens

Immunogen:

Uniprot:

Q8TE57(ATS16_HUMAN) >>Visit HPA database.

Gene(ID):

ADAMTS16(170690)

Expression:

Q8TE57 ATS16_HUMAN:

Expressed in fetal lung and kidney and in adult prostate and ovary.

Sequence:

Q8TE57 ATS16_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKPRARGWRGLAALWMLLAQVAEQAPACAMGPAAAAPGSPSVPRPPPPAERPGWMEKGEYDLVSAYEVDHRGDYVSHEIMHHQRRRRAVPVSEVESLHLRLKGSRHDFHMDLRTSSSLVAPGFIVQTLGKTGTKSVQTLPPEDFCFYQGSLRSHRNSSVALSTCQGLSGMIRTEEADYFLRPLPSHLSWKLGRAAQGSSPSHVLYKRSTEPHAPGASEVLVTSRTWELAHQPLHSSDLRLGLPQKQHFCGRRKKYMPQPPKEDLFILPDEYKSCLRHKRSLLRSHRNEELNVETLVVVDKKMMQNHGHENITTYVLTILNMVSALFKDGTIGGNINIAIVGLILLEDEQPGLVISHHADHTLSSFCQWQSGLMGKDGTRHDHAILLTGLDICSWKNEPCDTLGFAPISGMCSKYRSCTINEDTGLGLAFTIAHESGHNFGMIHDGEGNMCKKSEGNIMSPTLAGRNGVFSWSPCSRQYLHKFLSTAQAICLADQPKPVKEYKYPEKLPGELYDANTQCKWQFGEKAKLCMLDFKKDICKALWCHRIGRKCETKFMPAAEGTICGHDMWCRGGQCVKYGDEGPKPTHGHWSDWSSWSPCSRTCGGGVSHRSRLCTNPKPSHGGKFCEGSTRTLKLCNSQKCPRDSVDFRAAQCAEHNSRRFRGRHYKWKPYTQVEDQDLCKLYCIAEGFDFFFSLSNKVKDGTPCSEDSRNVCIDGICERVGCDNVLGSDAVEDVCGVCNGNNSACTIHRGLYTKHHHTNQYYHMVTIPSGARSIRIYEMNVSTSYISVRNALRRYYLNGHWTVDWPGRYKFSGTTFDYRRSYNEPENLIATGPTNETLIVELLFQGRNPGVAWEYSMPRLGTEKQPPAQPSYTWAIVRSECSVSCGGGQMTVREGCYRDLKFQVNMSFCNPKTRPVTGLVPCKVSACPPSWSVGNWSACSRTCGGGAQSRPVQCTRRVHYDSEPVPASLCPQPAPSSRQACNSQSCPPAWSAGPWAECSHTCGKGWRKRAVACKSTNPSARAQLLPDAVCTSEPKPRMHEACLLQRCHKPKKLQWLVSAWSQCSVTCERGTQKRFLKCAEKYVSGKYRELASKKCSHLPKPSLELERACAPLPCPRHPPFAAAGPSRGSWFASPWSQCTASCGGGVQTRSVQCLAGGRPASGCLLHQKPSASLACNTHFCPIAEKKDAFCKDYFHWCYLVPQHGMCSHKFYGKQCCKTCSKSNL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TE57 As Substrate

Q8TE57

Site	PTM Type	Source
S198	Phosphorylation	Uniprot
S201	Phosphorylation	Uniprot
S728	Phosphorylation	Uniprot
S1096	Phosphorylation	Uniprot
Y1211	Phosphorylation	Uniprot

Research Backgrounds

Q8TE57_ATS16_HUMAN

PTMs:

The precursor is cleaved by a furin endopeptidase.

Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Tissue Specificity:

Expressed in fetal lung and kidney and in adult prostate and ovary.

Family&Domains:

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

References

1). Association between the expression levels of ADAMTS16 and BMP2 and tumor budding in hepatocellular carcinoma. Oncology Letters, 2023 (PubMed: 37205917) [IF=2.5]

Application: IHC Species: Human Sample:

Figure 2. Immunohistochemical analysis of ADAMTS16 and BMP2 in patients with HCC with different TB statuses. (A) Representative IHC staining images of ADAMTS16 and BMP2 in TB-neg and TB-pos HCC tissues (magnification, ×100). Red boxes represent the tumor center and black arrows indicate budding tumor cells. (B) Representative IHC images of ADAMTS16 and BMP2 levels in the tumor center and budding cells (magnification, ×400), which are magnified views of the areas indicated by the red boxes and black arrows in (A), respectively. ADAMTS16, a metalloproteinase domain with thrombospondin motifs 16; BMP2, bone morphogenetic protein 2; HCC, hepatocellular carcinoma; IHC, immunohistochemistry; neg, negative; pos, positive; TB, tumor budding.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ADAMTS16 Antibody - #DF9173