CEL Antibody | Affinity Biosciences

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IF/ICC

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CEL Antibody - Western blot analysis of extracts from 293, using CEL Antibody.

CEL Antibody - DF9279 staining 3T3 by IF/ICC.

Product:	CEL Antibody
Catalog:	DF9279
Description:	Rabbit polyclonal antibody to CEL
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	79 kDa; 79kD(Calculated).
Uniprot:	P19835
RRID:	AB_2842475

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

CEL Antibody detects endogenous levels of total CEL.

RRID:

AB_2842475
Cite Format: Affinity Biosciences Cat# DF9279, RRID:AB_2842475.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BAL; Bile salt-stimulated lipase; BSDL; BSSL; Bucelipase; Carboxyl ester lipase (bile salt stimulated lipase); Carboxyl ester lipase; CEase; CEL; CELL; Cholesterol esterase; FAP; FAPP; Fetoacinar pancreatic protein; LIPA; Lysophospholipase, pancreatic; MODY8; Pancreatic lysophospholipase; Sterol esterase;

Immunogens

Immunogen:

Uniprot:

P19835(CEL_HUMAN) >>Visit HPA database.

Expression:

P19835 CEL_HUMAN:

Mammary gland and pancreas. Detected in pancreatic and duodenal juice (at protein level) (PubMed:21784842). Expressed by eosinophils.

Sequence:

P19835 CEL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGRLQLVVLGLTCCWAVASAAKLGAVYTEGGFVEGVNKKLGLLGDSVDIFKGIPFAAPTKALENPQPHPGWQGTLKAKNFKKRCLQATITQDSTYGDEDCLYLNIWVPQGRKQVSRDLPVMIWIYGGAFLMGSGHGANFLNNYLYDGEEIATRGNVIVVTFNYRVGPLGFLSTGDANLPGNYGLRDQHMAIAWVKRNIAAFGGDPNNITLFGESAGGASVSLQTLSPYNKGLIRRAISQSGVALSPWVIQKNPLFWAKKVAEKVGCPVGDAARMAQCLKVTDPRALTLAYKVPLAGLEYPMLHYVGFVPVIDGDFIPADPINLYANAADIDYIAGTNNMDGHIFASIDMPAINKGNKKVTEEDFYKLVSEFTITKGLRGAKTTFDVYTESWAQDPSQENKKKTVVDFETDVLFLVPTEIALAQHRANAKSAKTYAYLFSHPSRMPVYPKWVGADHADDIQYVFGKPFATPTGYRPQDRTVSKAMIAYWTNFAKTGDPNMGDSAVPTHWEPYTTENSGYLEITKKMGSSSMKRSLRTNFLRYWTLTYLALPTVTDQEATPVPPTGDSEATPVPPTGDSETAPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDAGPPPVPPTGDSGAPPVPPTGDSGAPPVTPTGDSETAPVPPTGDSGAPPVPPTGDSEAAPVPPTDDSKEAQMPAVIRF

PTMs - P19835 As Substrate

P19835

Site	PTM Type	Source
Y27	Phosphorylation	Uniprot
T28	Phosphorylation	Uniprot
S238	Phosphorylation	Uniprot
S245	Phosphorylation	Uniprot
Y365	Phosphorylation	Uniprot
T494	Phosphorylation	Uniprot
T558	O-Glycosylation	Uniprot
T569	O-Glycosylation	Uniprot
T579	O-Glycosylation	Uniprot
T607	O-Glycosylation	Uniprot
T618	O-Glycosylation	Uniprot
T629	O-Glycosylation	Uniprot
T640	O-Glycosylation	Uniprot
T651	O-Glycosylation	Uniprot
T662	O-Glycosylation	Uniprot
T673	O-Glycosylation	Uniprot

Research Backgrounds

P19835_CEL_HUMAN

Function:

Catalyzes the hydrolysis of a wide range of substrates including cholesteryl esters, phospholipids, lysophospholipids, di- and tri-acylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs). Preferentially hydrolyzes FAHFAs with the ester bond further away from the carboxylate. Unsaturated FAHFAs are hydrolyzed more quickly than saturated FAHFAs (By similarity). Has an essential role in the complete digestion of dietary lipids and their intestinal absorption, along with the absorption of fat-soluble vitamins.

PTMs:

N- and O-glycosylated.

Subcellular Location:

Secreted.

Tissue Specificity:

Mammary gland and pancreas. Detected in pancreatic and duodenal juice (at protein level). Expressed by eosinophils.

Subunit Structure:

Interacts with CLC.

Family&Domains:

Belongs to the type-B carboxylesterase/lipase family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CEL Antibody - #DF9279