BORG5 Antibody - #DF9356

(3)  
Product: BORG5 Antibody
Catalog: DF9356
Description: Rabbit polyclonal antibody to BORG5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 40~50 kDa; 40kD(Calculated).
Uniprot: Q00587
RRID: AB_2842552

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
BORG5 Antibody detects endogenous levels of total BORG5.
RRID:
AB_2842552
Cite Format: Affinity Biosciences Cat# DF9356, RRID:AB_2842552.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

55 kDa bone marrow stromal/endothelial cell protein; Binder of Rho GTPases 5; Bone marrow stromal/endothelial cell protein, 55-KD; BORG 5; BORG5; BORG5_HUMAN; CDC42 effector protein (Rho GTPase binding) 1; Cdc42 effector protein 1; Cdc42ep1; CEP 1; CEP1; MGC15316; MSE 55; MSE55; OTTHUMP00000028709; Serum constituent protein; Serum protein MSE55;

Immunogens

Immunogen:

A synthesized peptide derived from human BORG5, corresponding to a region within N-terminal amino acids.

Uniprot:

Q00587(BORG5_HUMAN) >>Visit HPA database.

Gene(ID):
CDC42EP1(11135)
Expression:
Q00587 BORG5_HUMAN:

Endothelial and bone marrow stromal cells.

Sequence:
MPGPQGGRGAATMSLGKLSPVGWVSSSQGKRRLTADMISHPLGDFRHTMHVGRGGDVFGDTSFLSNHGGSSGSTHRSPRSFLAKKLQLVRRVGAPPRRMASPPAPSPAPPAISPIIKNAISLPQLNQAAYDSLVVGKLSFDSSPTSSTDGHSSYGLDSGFCTISRLPRSEKPHDRDRDGSFPSEPGLRRSDSLLSFRLDLDLGPSLLSELLGVMSLPEAPAAETPAPAANPPAPTANPTGPAANPPATTANPPAPAANPSAPAATPTGPAANPPAPAASSTPHGHCPNGVTAGLGPVAEVKSSPVGGGPRGPAGPALGRHWGAGWDGGHHYPEMDARQERVEVLPQARASWESLDEEWRAPQAGSRTPVPSTVQANTFEFADAEEDDEVKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
88
Bovine
88
Sheep
88
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Probably involved in the organization of the actin cytoskeleton. Induced membrane extensions in fibroblasts.

Subcellular Location:

Endomembrane system>Peripheral membrane protein. Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Endothelial and bone marrow stromal cells.

Family&Domains:

The CRIB domain mediates interaction with CDC42.

Belongs to the BORG/CEP family.

Restrictive clause

 

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