Product: RNF123 Antibody
Catalog: DF9488
Description: Rabbit polyclonal antibody to RNF123
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 149 kDa; 149kD(Calculated).
Uniprot: Q5XPI4
RRID: AB_2842684

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RNF123 Antibody detects endogenous levels of total RNF123.
RRID:
AB_2842684
Cite Format: Affinity Biosciences Cat# DF9488, RRID:AB_2842684.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686C2222; E3 ubiquitin protein ligase RNF123; E3 ubiquitin-protein ligase RNF123; FLJ12565; FP1477; Kip1 ubiquitination promoting complex protein 1; Kip1 ubiquitination-promoting complex protein 1; KPC 1; KPC1; MGC163504; RING finger protein 123; RN123_HUMAN; RNF 123; Rnf123; Ubiquitin ligase;

Immunogens

Immunogen:

A synthesized peptide derived from human RNF123, corresponding to a region within N-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MASKGAGMSFSRKSYRLTSDAEKSRVTGIVQEKLLNDYLNRIFSSSEHAPPAATSRKPLNFQNLPEHLDQLLQVDNEEEESQGQVEGRLGPSTVVLDHTGGFEGLLLVDDDLLGVIGHSNFGTIRSTTCVYKGKWLYEVLISSQGLMQIGWCTISCRFNQEEGVGDTHNSYAYDGNRVRKWNVTTTNYGKAWAAGDIVSCLIDLDDGTLSFCLNGVSLGTAFENLSRGLGMAYFPAISLSFKESVAFNFGSRPLRYPVAGYRPLQDPPSADLVRAQRLLGCFRAVLSVELDPVEGRLLDKESSKWRLRGQPTVLLTLAHIFHHFAPLLRKVYLVEAVLMSFLLGIVEKGTPTQAQSVVHQVLDLLWLFMEDYEVQDCLKQLMMSLLRLYRFSPIVPDLGLQIHYLRLTIAILRHEKSRKFLLSNVLFDVLRSVVFFYIKSPLRVEEAGLQELIPTTWWPHCSSREGKESTEMKEETAEERLRRRAYERGCQRLRKRIEVVEELQVQILKLLLDNKDDNGGEASRYIFLTKFRKFLQENASGRGNMPMLCPPEYMVCFLHRLISALRYYWDEYKASNPHASFSEEAYIPPQVFYNGKVDYFDLQRLGGLLSHLRKTLKDDLASKANIVIDPLELQSTAMDDLDEDEEPAPAMAQRPMQALAVGGPLPLPRPGWLSSPTLGRANRFLSTAAVSLMTPRRPLSTSEKVKVRTLSVEQRTREDIEGSHWNEGLLLGRPPEEPEQPLTENSLLEVLDGAVMMYNLSVHQQLGKMVGVSDDVNEYAMALRDTEDKLRRCPKRRKDILAELTKSQKVFSEKLDHLSRRLAWVHATVYSQEKMLDIYWLLRVCLRTIEHGDRTGSLFAFMPEFYLSVAINSYSALKNYFGPVHSMEELPGYEETLTRLAAILAKHFADARIVGTDIRDSLMQALASYVCYPHSLRAVERIPEEQRIAMVRNLLAPYEQRPWAQTNWILVRLWRGCGFGYRYTRLPHLLKTKLEDANLPSLQKPCPSTLLQQHMADLLQQGPDVAPSFLNSVLNQLNWAFSEFIGMIQEIQQAAERLERNFVDSRQLKVCATCFDLSVSLLRVLEMTITLVPEIFLDWTRPTSEMLLRRLAQLLNQVLNRVTAERNLFDRVVTLRLPGLESVDHYPILVAVTGILVQLLVRGPASEREQATSVLLADPCFQLRSICYLLGQPEPPAPGTALPAPDRKRFSLQSYADYISADELAQVEQMLAHLTSASAQAAAASLPTSEEDLCPICYAHPISAVFQPCGHKSCKACINQHLMNNKDCFFCKTTIVSVEDWEKGANTSTTSSAA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Promotes the ubiquitination and proteasome-mediated degradation of CDKN1B which is the cyclin-dependent kinase inhibitor at the G0-G1 transition of the cell cycle. Functions also as an inhibitor of innate antiviral signaling mediated by DDX58 and IFIH1 independently of its E3 ligase activity. Interacts with the N-terminal CARD domains of DDX58 and IFIH1 and competes with the downstream adapter MAVS.

PTMs:

Ubiquitinated, leading to its degradation. Deubiquitinated by USP19, thereby stimulating CDKN1B ubiquitin-dependent degradation.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

References

1). RNF123 Mediates Ubiquitination and Degradation of SOCS1 To Regulate Type I Interferon Production during Duck Tembusu Virus Infection. Journal of Virology, 2023 (PubMed: 37014223) [IF=4.0]

Application: WB    Species: Human    Sample: HEK 293T cells

FIG 2 RNF123 binds to the SH2 domain of SOCS1 through its RING domain and promotes the K48-linked ubiquitination of SOCS1. (A) Mass spectroscopic analysis of potential SOCS1 binding proteins. The numbers of peptides recognized in interacting proteins (including RNF123) are shown. (B and C) HEK 293T cells were transfected with pCAGGS-RNF123-Flag (1.5 mg/well) and pCAGGS (1.5 mg/well), pCAGGS and pCAGGS-SOCS1-Myc (1.5 mg/well), and pCAGGS-RNF123-Flag and pCAGGS-SOCS1-Myc. Thirty-six hours later, the cell lysates were immunoprecipitated with anti-Myc or anti-Flag, followed by IB with anti-Myc and anti-Flag. (D) When the cell confluence reached about 90%, the cells were infected with DTMUV at an MOI of 1. Thirty-six hours after infection, the cells were collected for indirect immunofluorescence detection of endogenous SOCS1 and RNF123 colocalization (original magnification, ×600). (E and F) DEFs were infected with DTMUV at an MOI of 1. Thirty-six hours later, the cell lysates were immunoprecipitated with anti-SOCS1, anti-RNF123, or IgG, followed by IB with anti-SOCS1 and anti-RNF123. (G and H) HEK 293T cells were transfected with pCAGGS-RNF123-Flag (1.5 mg/well) together with the plasmids for full-length or truncated SOCS1 (1.5 mg/well). Thirty-six hours later, the cell lysates were immunoprecipitated with anti-Myc or anti-Flag, followed by IB with anti-Myc and anti-Flag. (I) Schematic representation of plasmids for full-length (FL) and truncated RNF123. (J and K) HEK 293T cells were transfected with pCAGGS-SOCS1-Myc (1.5 mg/well) together with the plasmids for full-length or truncated RNF123 (1.5 mg/well). Thirty-six hours later, the cell lysates were immunoprecipitated with anti-Myc or anti-Flag, followed by IB with anti-Myc and anti-Flag. (L) HEK 293T cells were transfected with HA-Ub-K48 (1 mg/well) together with pCAGGS-SOCS1-Myc (1 mg/well) or pCAGGS-RNF123-Flag (1 mg/well) and pCAGGS-SOCS1-Myc. Thirty-six hours after transfection, the cell lysates were immunoprecipitated with anti-Myc, and ubiquitinated SOCS1 was detected by IB with anti-HA. (M) HEK 293T cells were transfected with pCAGGS-SOCS1-Myc (1 mg/well) and HA-Ub-K48 (1 mg/well) together with shRNA-RNF123 (1 mg/well) or shRNA-NC (blank control of shRNA-RNF123). Thirty-six hours after transfection, cells were treated with MG132 (10 μM) or DMSO for 6 h. The cell lysates were immunoprecipitated with anti-Myc, and ubiquitinated SOCS1 was detected by IB with anti-HA. (N) HEK 293T cells were transfected with pCAGGS-RNF123-Flag (1 mg/well) and HA-Ub-K48 (1 mg/well) together with the WT, the K114R or K137R single-point mutant, or the DM (1 mg/well). Thirty-six hours after transfection, the cell lysates were immunoprecipitated with anti-Myc, and ubiquitinated SOCS1 was detected by IB with anti-HA. All data are the mean values obtained from at least three replicates of independent experiments.

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